Name: feeding experimentation device (fed): construction and validation of an open-source device for measuring food intake in rodents
Uploaded: 2017-02-21T14:00:00
Description: Watch this Scientific Journal Video about Feeding Experimentation Device FED: Construction and Validation of an Open-source Device for Measuring Food Intake in Rodents at JoVE.com

这项工作是由美国国立卫生研究院院内研究计划（NIH），国家糖尿病研究所，消化道和肾脏疾病（NIDDK）的支持。我们感谢对仪表的NIH科和美国国立卫生研究院图书馆与3D打印的援助。

注：此协议是在材料的表命名的专门组件写入。虽然可以使用其他硬件来实现类似的功能，FED已编程的Arduino的专业微控制器（以下称为：微控制器）和上市附件。其它微控制器可以同样出色的工作，但会要求用户修改代码来支持它们。离线数据分析使用Python编程语言编码。 1.准备和软件安装<ol><li>采购，构建FED（ 见表1 andFed Github上BoM.xlxs在：https://github.com/KravitzLab/FED/tree/master/...

<ol>
	<li>Ellacott, K. L., Morton, G. J., Woods, S. C., Tso, P., Schwartz, M. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assessment+of+feeding+behavior+in+laboratory+mice.">Assessment of feeding behavior in laboratory mice.</a> Cell Metab. 12 (1), 10-17 (2010).</li><li>Betley, J. N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neurons+for+hunger+and+thirst+transmit+a+negative-valence+teaching+signal.">Neurons for hunger and thirst transmit a negative-valence teaching signal.</a> Nature. 521 (7551), 180-185 (2015).</li><li>van den Heuvel, J. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neuropeptide+Y+activity+in+the+nucleus+accumbens+modulates+feeding+behavior+and+neuronal+activity.">Neuropeptide Y activity in the nucleus accumbens modulates feeding behavior and neuronal activity.</a> Biol Psychiatry. 77 (7), 633-641 (2015).</li><li>Cone, J. J., Roitman, J. D., Roitman, M. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ghrelin+regulates+phasic+dopamine+and+nucleus+accumbens+signaling+evoked+by+food-predictive+stimuli.">Ghrelin regulates phasic dopamine and nucleus accumbens signaling evoked by food-predictive stimuli.</a> J Neurochem. 133 (6), 844-856 (2015).</li><li>Ulman, E. A., Compton, D., Kochanek, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Measuring+food+and+water+intake+in+rats+and+mice.">Measuring food and water intake in rats and mice.</a> ALN Mag. , 17-20 (2008).</li><li>Nguyen, K. P., O'Neal, T. J., Bolonduro, O. A., White, E., Kravitz, A. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Feeding+Experimentation+Device+(FED):+A+flexible+open-source+device+for+measuring+feeding+behavior.">Feeding Experimentation Device (FED): A flexible open-source device for measuring feeding behavior.</a> J Neurosci Methods. 267, 108-114 (2016).</li><li>Aguiar, P., Mendonca, L., Galhardo, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=OpenControl:+a+free+opensource+software+for+video+tracking+and+automated+control+of+behavioral+mazes.">OpenControl: a free opensource software for video tracking and automated control of behavioral mazes.</a> J Neurosci Methods. 166 (1), 66-72 (2007).</li><li>Devarakonda, K., Nguyen, K. P., Kravitz, A. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=ROBucket:+A+low+cost+operant+chamber+based+on+the+Arduino+microcontroller.">ROBucket: A low cost operant chamber based on the Arduino microcontroller.</a> Behav Res Methods. 48 (2), 503-509 (2016).</li><li>Hoffman, A. M., Song, J., Tuttle, E. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=ELOPTA:+a+novel+microcontroller-based+operant+device.">ELOPTA: a novel microcontroller-based operant device.</a> Behav Res Methods. 39 (4), 776-782 (2007).</li><li>Crall, J. D., Gravish, N., Mountcastle, A. M., Combes, S. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=BEEtag:+A+Low-Cost,+Image-Based+Tracking+System+for+the+Study+of+Animal+Behavior+and+Locomotion.">BEEtag: A Low-Cost, Image-Based Tracking System for the Study of Animal Behavior and Locomotion.</a> PLoS One. 10 (9), (2015).</li></ol>

喂养实验设备（FED）是一种灵活的食物摄入量监测系统。在这里，我们描述了在制造和诊断设备，包括3D打印硬件，电子元件焊接，和素描上传的组装到微控制器的详细说明。虽然它遵循谨慎协议列出的所有步骤是很重要的，也有值得特别注意在每个部分，以确保一个成功的终端产品的关键步骤。三维印刷旋转盘应紧贴到步进电机轴，并能够以最小的阻力来自相邻部件旋转。当焊接引脚连接，确?...

随着全球肥胖的兴起在20 世纪后期，有再度上下层饲养1，2，3，4机制的关注。通常，食物摄入量是手动称量5，或用市售的馈送系统。商业系统都很出色，但在修改其设计或代码提供有限的灵活性。在这里，我们描述了实验饲养设备（FED）：用于测量食物的摄入量以优良的时间分辨率和最小的人为干扰6的开源料系统。 FED是电池供电，可以容纳标准的殖民地齿条笼或其他科学仪器内部的3D打印的情况下，在完全包含。 在稳态下，FED在低功耗模式下工作，在其FO食物丸安息OD好。粒料的存在通过红外光束进行监测。当鼠标移除粒料，一个光断续传感器将信号发送到微控制器和时间标记被记录板载安全数字（SD）卡上。同时，晶体管 - 晶体管逻辑（TTL）输出提供粒料检索的实时输出。紧接这种情况下，马达旋转以分配另一个粒料，并且系统返回到其低功率模式。由于它的开源本质，美联储可以进行修改和完善，以适应特定的研究需求。例如，代码可以容易地改变，以进料限制在每天的特定时间，或停止分配时已经达到一个数量的颗粒，而不需要人为干预。 在这里，我们概括为建筑，验证和使用FED的一步一步的说明在小鼠体内测量食物的摄入。我们提供的所有组件的列表来构造一个系统。重要的是，没有事先恩在电子perience需要来构造的FED。

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Electronics</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Adafruit Motor/Stepper/Servo Shield for Arduino v2 Kit - v2.3</td>
			<td>Adafruit</td>
			<td>1438</td>
			<td>Use of other motor shields has not been tested and will require changes to the code</td>
		</tr>
		<tr>
			<td>Adafruit Assembled Data Logging shield for Arduino</td>
			<td>Adafruit</td>
			<td>1141</td>
			<td>Use of other data logging shields has not been tested and will require changes to the code</td>
		</tr>
		<tr>
			<td>PowerBoost 500 Charger</td>
			<td>Adafruit</td>
			<td>1944</td>
			<td>Other voltge regulator boards have not been tested, but should work if they have similar specifications</td>
		</tr>
		<tr>
			<td>FTDI Friend + extras - v1.0</td>
			<td>Adafruit</td>
			<td>284</td>
			<td>Any FTDI-USB connection will work</td>
		</tr>
		<tr>
			<td>Small Reduction Stepper Motor - 5VDC 32-Step 1/16 Gearing</td>
			<td>Adafruit</td>
			<td>858</td>
			<td>Use of other stepper motors has not been tested</td>
		</tr>
		<tr>
			<td>Arduino Pro 328 - 5V/16MHz</td>
			<td>SparkFun</td>
			<td>DEV-10915</td>
			<td>Other Arduino boards should work, although may require changes to the code</td>
		</tr>
		<tr>
			<td>Photo Interrupter - GP1A57HRJ00F</td>
			<td>SparkFun</td>
			<td>SEN-09299</td>
			<td>Other photointerrupters will work, but may require changes to the 3D design</td>
		</tr>
		<tr>
			<td>SparkFun Photo Interrupter Breakout Board - GP1A57HRJ00F</td>
			<td>SparkFun</td>
			<td>BOB-09322</td>
			<td>Other photointerrupters will work, but may require changes to the 3D design</td>
		</tr>
		<tr>
			<td>Connectors, screws, and miscellaneous items</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Shield stacking headers for Arduino (R3 Compatible)</td>
			<td>Adafruit</td>
			<td>85</td>
			<td>Any stacking header that says Arduiono R3 compatible will work</td>
		</tr>
		<tr>
			<td>Multi-Colored Heat Shrink Pack - 3/32&#34; + 1/8&#34; + 3/16&#34;</td>
			<td>Adafruit</td>
			<td>1649</td>
			<td>Any heatshrink will work</td>
		</tr>
		<tr>
			<td>Hook-up Wire Spool Set - 22AWG Solid Core - 6x25ft</td>
			<td>Adafruit</td>
			<td>1311</td>
			<td>Any wire will work</td>
		</tr>
		<tr>
			<td>Lithium Ion Battery Pack - 3.7V 4400mAh</td>
			<td>Adafruit</td>
			<td>354</td>
			<td>Any 3.7V Lithium battery with a JST connector will work&#160;</td>
		</tr>
		<tr>
			<td>SD/MicroSD Memory Card (8GB SDHC)</td>
			<td>Adafruit</td>
			<td>1294</td>
			<td>Any SD card will work</td>
		</tr>
		<tr>
			<td>50 Ohm BNC Bulkhead Jack (3/8&#34; D-Hole)</td>
			<td>L-com</td>
			<td>BAC70A</td>
			<td>Any BNC bulkhead will work</td>
		</tr>
		<tr>
			<td>Type 316 Stainless Steel Pan Head Phillips Sheet metal screw, No 6 size, 1/4&#34; Length</td>
			<td>McMaster-Carr</td>
			<td>90184A120</td>
			<td>Any screws of this specification will work</td>
		</tr>
		<tr>
			<td>Type 316 Stainless Steel Pan Head Phillips Sheet metal screw, No 2 size, 1/4&#34; Length</td>
			<td>McMaster-Carr</td>
			<td>91735A102</td>
			<td>Any screws of this specification will work</td>
		</tr>
		<tr>
			<td>Nylon 100 Degree Flat Head Slotted Machine Screw, 4-40 Thread, 1&#34; Length</td>
			<td>McMaster-Carr</td>
			<td>90241A253</td>
			<td>Any screws of this specification will work</td>
		</tr>
		<tr>
			<td>Nylon Hex Nut, 4-40 Thread Size</td>
			<td>McMaster-Carr</td>
			<td>94812A200</td>
			<td>Any nut of this specification will work</td>
		</tr>
		<tr>
			<td>2Pin JST M F Connector 200mm 22AWG Wire Cable</td>
			<td>NewEgg</td>
			<td>9SIA27C3FY2876</td>
			<td>Any 2 pin connector will work for this connection</td>
		</tr>
		<tr>
			<td>Metal Pushbutton - Latching (16mm, Red)</td>
			<td>SparkFun</td>
			<td>COM-11971</td>
			<td>Any push button or switch will work</td>
		</tr>
		<tr>
			<td>Resistor Kit - 1/4W</td>
			<td>SparkFun</td>
			<td>COM-10969</td>
			<td>Any 1/4W resistors will work</td>
		</tr>
	</tbody>
</table>

feeding experimentation device (fed): construction and validation of an open-source device for measuring food intake in rodents

Food intake measurements are essential for many research studies. Here, we provide a detailed description of a novel solution for measuring food intake in mice: the Feeding Experimentation Device (FED). FED is an open-source system that was designed to facilitate flexibility in food intake studies. Due to its compact and battery powered design, FED can be placed within standard home cages or other experimental equipment. Food intake measurements can also be synchronized with other equipment in real-time via FED's transistor-transistor logic (TTL) digital output, or in post-acquisition processing as FED timestamps every event with a real-time clock. When in use, a food pellet sits within FED's food well where it is monitored via an infrared beam. When the pellet is removed by the mouse, FED logs the timestamp onto its internal secure digital (SD) card and dispenses another pellet. FED can run for up to 5 days before it is necessary to charge the battery and refill the pellet hopper, minimizing human interference in data collection. Assembly of FED requires minimal engineering background, and off-the-shelf materials and electronics were prioritized in its construction. We also provide scripts for analysis of food intake and meal patterns. Finally, FED is open-source and all design and construction files are online, to facilitate modifications and improvements by other researchers.

涉及使用动物的验证试验，审查和糖尿病研究所，消化和肾脏疾病的动物护理和使用委员会的批准。为了说明如何使用FED的用于测量家笼喂养，成年雌性C57BL / 6小鼠（n = 4）单独圈养用12/12小时光照/黑暗循环下以水和标准实验室食物随意访问（灯上05:00）。后一个星期习惯期，除去粮食料斗，并用FED置换为验证测试​​的五天。在测试期结束时，场发射，从笼...

Watch this Scientific Journal Video about Feeding Experimentation Device FED: Construction and Validation of an Open-source Device for Measuring Food Intake in Rodents at JoVE.com

Feeding Experimentation Device FED: Construction and Validation of an Open-source Device for Measuring Food Intake in Rodents (Video) | JoVE

Feeding Experimentation Device (FED) is an open-source device for measuring food intake in mice. FED can also synchronize food intake measurements with other techniques via a real-time digital output. Here, we provide a step-by-step tutorial for the construction, validation, and usage of FED.

饲养实验装置（FED）：建筑和验​​证的开源设备的在鼠害测量摄食

Food intake measurements are essential for many research studies. Here, we provide a detailed description of a novel solution for measuring food intake in ...

Research

JoVE Journal

Behavior

Feeding Experimentation Device (FED): Construction and Validation of an Open-source Device for Measuring Food Intake in Rodents

Use of the Operant Orofacial Pain Assessment Device (OPAD) to Measure Changes in Nociceptive Behavior

Use of the Operant Orofacial Pain Assessment Device OPAD to Measure Changes in Nociceptive Behavior (Video) | JoVE

使用操作性口面疼痛评估设备（OPAD）测量伤害行为的变化

We present an operant  system for the detection of pain in awake, conscious rodents. The Orofacial  Pain Assessment Device (OPAD) assesses pain behaviors ...

科研

行为学

A Procedure to Study the Effect of Prolonged Food Restriction on Heroin Seeking in Abstinent Rats

A Procedure to Study the Effect of Prolonged Food Restriction on Heroin Seeking in Abstinent Rats (Video) | JoVE

一个过程来研究延长食品的限制对海洛因寻求戒断大鼠的影响

In human drug addicts, exposure to drug-associated cues or environments that were previously associated with drug taking can trigger relapse during ...

Studying Food Reward and Motivation in Humans

Studying Food Reward and Motivation in Humans (Video) | JoVE

研究食品奖赏和动机在人类

A key challenge in studying reward processing in humans is to go beyond subjective self-report measures and quantify different aspects of reward such as ...

Assessment of Social Cognition in Non-human Primates Using a Network of Computerized Automated Learning Device (ALDM) Test Systems

Assessment of Social Cognition in Non-human Primates Using a Network of Computerized Automated Learning Device ALDM Test Systems (Video) | JoVE

社会认知的非人类灵长类动物评估使用电脑自动学习设备（ALDM）测试系统的网络

Fagot &#38; Paleressompoulle1 and Fagot &#38; Bonte2 have published an automated learning device (ALDM) for the study of cognitive abilities of monkeys ...

An Unpredictable Chronic Mild Stress Protocol for Instigating Depressive Symptoms, Behavioral Changes and Negative Health Outcomes in Rodents

An Unpredictable Chronic Mild Stress Protocol for Instigating Depressive Symptoms, Behavioral Changes and Negative Health Outcomes in Rodents (Video) | JoVE

煽动抑郁症状，行为变化和不利健康结果鼠害一个不可预知的慢性轻度应激协议

Chronic, unresolved stress is a major risk factor for the development of clinical depression. While many preclinical models of stress-induced depression ...

Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila

Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila (Video) | JoVE

在果蝇中结合定量食物摄入量测定和强迫激活神经元研究食欲

Food consumption is under the tight control of the brain, which integrates the physiological status, palatability, and nutritional contents of the food, ...

An Automated T-maze Based Apparatus and Protocol for Analyzing Delay- and Effort-based Decision Making in  Free Moving Rodents

An Automated T-maze Based Apparatus and Protocol for Analyzing Delay- and Effort-based Decision Making in  Free Moving Rodents (Video) | JoVE

基于自动 T 迷宫的自由移动啮齿动物延迟和努力决策分析装置及协议

Many neurological and psychiatric patients demonstrate difficulties and/or deficits in decision making. Rodent models are helpful to produce a deeper ...

A Vibrotactile Feedback Device for Seated Balance Assessment and Training

A Vibrotactile Feedback Device for Seated Balance Assessment and Training (Video) | JoVE

一种用于座位平衡评估和训练的振动反馈装置

Postural perturbations, motion tracking, and sensory feedback are modern techniques used to challenge, assess, and train upright sitting, respectively. ...

In Vivo Protocol of Controlled Subconcussive Head Impacts for the Validation of Field Study Data

In Vivo Protocol of Controlled Subconcussive Head Impacts for the Validation of Field Study Data (Video) | JoVE

控制下冲击头冲击的活体协议对现场研究数据的验证

Subconcussive hits pose a threat to neuronal health as they have shown to induce neuronal structural damage and functional impairment without causing ...

Providing Meaningful Environmental Enrichment and Measuring Saliva Cortisol in Pigs Housed on Slatted Flooring

Providing Meaningful Environmental Enrichment and Measuring Saliva Cortisol in Pigs Housed on Slatted Flooring (Video) | JoVE

提供有意义的环境富集和测量唾液皮质醇在猪安置在板状地板

As pork is the most consumed meat worldwide, the welfare of animals in the swine industry has increasingly become a major public concern, which imposes a ...

饲养实验装置（FED）：建筑和验​​证的开源设备的在鼠害测量摄食

饲养实验装置（FED）：建筑和验证的开源设备的在鼠害测量摄食