Name: feeding experimentation device (fed): construction and validation of an open-source device for measuring food intake in rodents
Uploaded: 2017-02-21T14:00:00
Description: Watch this Scientific Journal Video about Feeding Experimentation Device FED: Construction and Validation of an Open-source Device for Measuring Food Intake in Rodents at JoVE.com

Dit werk werd ondersteund door de Intramurale Research Program van de National Institutes of Health (NIH), het National Institute of Diabetes en spijsvertering en Kidney Diseases (NIDDK). Wij danken de NIH Sectie op Instrumentatie en de NIH bibliotheek voor hulp bij het 3D-printen.

LET OP: Dit protocol is geschreven voor componenten met name in de tabel van Materialen genoemd. Terwijl vergelijkbare functionaliteit kan worden bereikt met andere hardware, werd FED geprogrammeerd voor de Arduino Pro microcontroller (voortaan genoemd: microcontroller) en beursgenoteerde accessoires. Andere microcontrollers kan even goed werken, maar zal de gebruiker vereisen om de code om hen te steunen aan te passen. Offline data-analyse werd gecodeerd met behulp van de programmeertaal Python. 1. Voorbereiding ...

<ol>
	<li>Ellacott, K. L., Morton, G. J., Woods, S. C., Tso, P., Schwartz, M. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assessment+of+feeding+behavior+in+laboratory+mice.">Assessment of feeding behavior in laboratory mice.</a> Cell Metab. 12 (1), 10-17 (2010).</li><li>Betley, J. N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neurons+for+hunger+and+thirst+transmit+a+negative-valence+teaching+signal.">Neurons for hunger and thirst transmit a negative-valence teaching signal.</a> Nature. 521 (7551), 180-185 (2015).</li><li>van den Heuvel, J. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neuropeptide+Y+activity+in+the+nucleus+accumbens+modulates+feeding+behavior+and+neuronal+activity.">Neuropeptide Y activity in the nucleus accumbens modulates feeding behavior and neuronal activity.</a> Biol Psychiatry. 77 (7), 633-641 (2015).</li><li>Cone, J. J., Roitman, J. D., Roitman, M. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ghrelin+regulates+phasic+dopamine+and+nucleus+accumbens+signaling+evoked+by+food-predictive+stimuli.">Ghrelin regulates phasic dopamine and nucleus accumbens signaling evoked by food-predictive stimuli.</a> J Neurochem. 133 (6), 844-856 (2015).</li><li>Ulman, E. A., Compton, D., Kochanek, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Measuring+food+and+water+intake+in+rats+and+mice.">Measuring food and water intake in rats and mice.</a> ALN Mag. , 17-20 (2008).</li><li>Nguyen, K. P., O'Neal, T. J., Bolonduro, O. A., White, E., Kravitz, A. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Feeding+Experimentation+Device+(FED):+A+flexible+open-source+device+for+measuring+feeding+behavior.">Feeding Experimentation Device (FED): A flexible open-source device for measuring feeding behavior.</a> J Neurosci Methods. 267, 108-114 (2016).</li><li>Aguiar, P., Mendonca, L., Galhardo, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=OpenControl:+a+free+opensource+software+for+video+tracking+and+automated+control+of+behavioral+mazes.">OpenControl: a free opensource software for video tracking and automated control of behavioral mazes.</a> J Neurosci Methods. 166 (1), 66-72 (2007).</li><li>Devarakonda, K., Nguyen, K. P., Kravitz, A. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=ROBucket:+A+low+cost+operant+chamber+based+on+the+Arduino+microcontroller.">ROBucket: A low cost operant chamber based on the Arduino microcontroller.</a> Behav Res Methods. 48 (2), 503-509 (2016).</li><li>Hoffman, A. M., Song, J., Tuttle, E. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=ELOPTA:+a+novel+microcontroller-based+operant+device.">ELOPTA: a novel microcontroller-based operant device.</a> Behav Res Methods. 39 (4), 776-782 (2007).</li><li>Crall, J. D., Gravish, N., Mountcastle, A. M., Combes, S. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=BEEtag:+A+Low-Cost,+Image-Based+Tracking+System+for+the+Study+of+Animal+Behavior+and+Locomotion.">BEEtag: A Low-Cost, Image-Based Tracking System for the Study of Animal Behavior and Locomotion.</a> PLoS One. 10 (9), (2015).</li></ol>

De Feeding Experimentation Device (FED) is een flexibele voedselinname bewakingssysteem. We beschrijven hier gedetailleerde instructies voor het vervaardigen en oplossen van problemen met het apparaat, met inbegrip van de assemblage van 3D geprint hardware, solderen van elektrische onderdelen, en het uploaden van sketches op de microcontrollers. Hoewel het belangrijk om alle stappen in het protocol nauwgezet te volgen, zijn er kritische stappen die extra aandacht verdienen in elke sectie tot een goed einde product. De 3...

Met de opkomst van de wereldwijde obesitas over de tweede helft van de 20e eeuw, is er hernieuwde aandacht op de mechanismen die ten grondslag liggen aan het voeden van 1, 2, 3, 4. Typisch wordt voedselinname handmatig gewogen 5 of met commercieel verkrijgbare voersystemen. Commerciële systemen zijn uitstekend, maar bieden beperkte flexibiliteit in het wijzigen van hun ontwerpen of code. Hier beschrijven we de Feeding Experimentation Device (FED): een open-source voersysteem voor het meten van de voedselinname met fijne tijdsresolutie en minimale menselijke tussenkomst 6. FED is op batterijen en volledig opgenomen in een 3D geprint koffer die passen binnen standaard kolonie rack kooien of andere wetenschappelijke apparatuur. In de stationaire toestand, FED opereert in een low-power mode met een voedsel pellet rusten in de food goed. De aanwezigheid van de pellet wordt via een infraroodstraal. Wanneer een muis een pellet verwijdert, een foto-onderbreker sensor stuurt een signaal naar de microcontroller en de tijd-stempel is aangemeld op de onboard Secure Digital (SD) kaart. Tegelijkertijd, een transistor-transistor logica (TTL) uitgang biedt een real-time productie van pellets retrieval. Onmiddellijk na deze gebeurtenis, de motor draait op een andere pellet af te geven, en het systeem keert terug naar de low power mode. Door zijn open-source karakter, kan FED worden aangepast en verbeterd om specifiek onderzoek behoeften. Bijvoorbeeld kan de code eenvoudig veranderd om voeding te beperken tot bepaalde tijden van de dag of stoppen afgeven wanneer verschillende pellets bereikt worden, zonder menselijke tussenkomst. Hier, schetsen we de stap-voor-stap instructies voor de bouw, de validatie, en het gebruik van de FED voor het meten van voedselinname bij muizen. Wij bieden een overzicht van alle componenten om een ​​systeem te bouwen. Belangrijk is dat geen voorafgaande exvaring in de elektronica nodig te bouwen FED.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Electronics</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Adafruit Motor/Stepper/Servo Shield for Arduino v2 Kit - v2.3</td>
			<td>Adafruit</td>
			<td>1438</td>
			<td>Use of other motor shields has not been tested and will require changes to the code</td>
		</tr>
		<tr>
			<td>Adafruit Assembled Data Logging shield for Arduino</td>
			<td>Adafruit</td>
			<td>1141</td>
			<td>Use of other data logging shields has not been tested and will require changes to the code</td>
		</tr>
		<tr>
			<td>PowerBoost 500 Charger</td>
			<td>Adafruit</td>
			<td>1944</td>
			<td>Other voltge regulator boards have not been tested, but should work if they have similar specifications</td>
		</tr>
		<tr>
			<td>FTDI Friend + extras - v1.0</td>
			<td>Adafruit</td>
			<td>284</td>
			<td>Any FTDI-USB connection will work</td>
		</tr>
		<tr>
			<td>Small Reduction Stepper Motor - 5VDC 32-Step 1/16 Gearing</td>
			<td>Adafruit</td>
			<td>858</td>
			<td>Use of other stepper motors has not been tested</td>
		</tr>
		<tr>
			<td>Arduino Pro 328 - 5V/16MHz</td>
			<td>SparkFun</td>
			<td>DEV-10915</td>
			<td>Other Arduino boards should work, although may require changes to the code</td>
		</tr>
		<tr>
			<td>Photo Interrupter - GP1A57HRJ00F</td>
			<td>SparkFun</td>
			<td>SEN-09299</td>
			<td>Other photointerrupters will work, but may require changes to the 3D design</td>
		</tr>
		<tr>
			<td>SparkFun Photo Interrupter Breakout Board - GP1A57HRJ00F</td>
			<td>SparkFun</td>
			<td>BOB-09322</td>
			<td>Other photointerrupters will work, but may require changes to the 3D design</td>
		</tr>
		<tr>
			<td>Connectors, screws, and miscellaneous items</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Shield stacking headers for Arduino (R3 Compatible)</td>
			<td>Adafruit</td>
			<td>85</td>
			<td>Any stacking header that says Arduiono R3 compatible will work</td>
		</tr>
		<tr>
			<td>Multi-Colored Heat Shrink Pack - 3/32&#34; + 1/8&#34; + 3/16&#34;</td>
			<td>Adafruit</td>
			<td>1649</td>
			<td>Any heatshrink will work</td>
		</tr>
		<tr>
			<td>Hook-up Wire Spool Set - 22AWG Solid Core - 6x25ft</td>
			<td>Adafruit</td>
			<td>1311</td>
			<td>Any wire will work</td>
		</tr>
		<tr>
			<td>Lithium Ion Battery Pack - 3.7V 4400mAh</td>
			<td>Adafruit</td>
			<td>354</td>
			<td>Any 3.7V Lithium battery with a JST connector will work&#160;</td>
		</tr>
		<tr>
			<td>SD/MicroSD Memory Card (8GB SDHC)</td>
			<td>Adafruit</td>
			<td>1294</td>
			<td>Any SD card will work</td>
		</tr>
		<tr>
			<td>50 Ohm BNC Bulkhead Jack (3/8&#34; D-Hole)</td>
			<td>L-com</td>
			<td>BAC70A</td>
			<td>Any BNC bulkhead will work</td>
		</tr>
		<tr>
			<td>Type 316 Stainless Steel Pan Head Phillips Sheet metal screw, No 6 size, 1/4&#34; Length</td>
			<td>McMaster-Carr</td>
			<td>90184A120</td>
			<td>Any screws of this specification will work</td>
		</tr>
		<tr>
			<td>Type 316 Stainless Steel Pan Head Phillips Sheet metal screw, No 2 size, 1/4&#34; Length</td>
			<td>McMaster-Carr</td>
			<td>91735A102</td>
			<td>Any screws of this specification will work</td>
		</tr>
		<tr>
			<td>Nylon 100 Degree Flat Head Slotted Machine Screw, 4-40 Thread, 1&#34; Length</td>
			<td>McMaster-Carr</td>
			<td>90241A253</td>
			<td>Any screws of this specification will work</td>
		</tr>
		<tr>
			<td>Nylon Hex Nut, 4-40 Thread Size</td>
			<td>McMaster-Carr</td>
			<td>94812A200</td>
			<td>Any nut of this specification will work</td>
		</tr>
		<tr>
			<td>2Pin JST M F Connector 200mm 22AWG Wire Cable</td>
			<td>NewEgg</td>
			<td>9SIA27C3FY2876</td>
			<td>Any 2 pin connector will work for this connection</td>
		</tr>
		<tr>
			<td>Metal Pushbutton - Latching (16mm, Red)</td>
			<td>SparkFun</td>
			<td>COM-11971</td>
			<td>Any push button or switch will work</td>
		</tr>
		<tr>
			<td>Resistor Kit - 1/4W</td>
			<td>SparkFun</td>
			<td>COM-10969</td>
			<td>Any 1/4W resistors will work</td>
		</tr>
	</tbody>
</table>

feeding experimentation device (fed): construction and validation of an open-source device for measuring food intake in rodents

Food intake measurements are essential for many research studies. Here, we provide a detailed description of a novel solution for measuring food intake in mice: the Feeding Experimentation Device (FED). FED is an open-source system that was designed to facilitate flexibility in food intake studies. Due to its compact and battery powered design, FED can be placed within standard home cages or other experimental equipment. Food intake measurements can also be synchronized with other equipment in real-time via FED's transistor-transistor logic (TTL) digital output, or in post-acquisition processing as FED timestamps every event with a real-time clock. When in use, a food pellet sits within FED's food well where it is monitored via an infrared beam. When the pellet is removed by the mouse, FED logs the timestamp onto its internal secure digital (SD) card and dispenses another pellet. FED can run for up to 5 days before it is necessary to charge the battery and refill the pellet hopper, minimizing human interference in data collection. Assembly of FED requires minimal engineering background, and off-the-shelf materials and electronics were prioritized in its construction. We also provide scripts for analysis of food intake and meal patterns. Finally, FED is open-source and all design and construction files are online, to facilitate modifications and improvements by other researchers.

Valideringsproeven die het gebruik van dieren werden beoordeeld en goedgekeurd door de Animal Care en gebruik Comite van het National Institute of Diabetes en spijsvertering en Kidney Diseases goedgekeurd. Om het gebruik van de FED te tonen voor het meten van kooi voeden, volwassen vrouwelijke C57BL / 6 muizen (n = 4) werden individueel gehuisvest met ad libitum toegang tot water en standaard laboratorium chow onder een 12/12 uur licht / donker-cyclus (lichten aan om 05...

Watch this Scientific Journal Video about Feeding Experimentation Device FED: Construction and Validation of an Open-source Device for Measuring Food Intake in Rodents at JoVE.com

Feeding Experimentation Device FED: Construction and Validation of an Open-source Device for Measuring Food Intake in Rodents

Feeding Experimentation Device (FED) is an open-source device for measuring food intake in mice. FED can also synchronize food intake measurements with other techniques via a real-time digital output. Here, we provide a step-by-step tutorial for the construction, validation, and usage of FED.

Feeding Experimentation Device (FED): Bouw en Validatie van een open-source-apparaat voor het meten van voedselinname bij knaagdieren

Food intake measurements are essential for many research studies. Here, we provide a detailed description of a novel solution for measuring food intake in ...

Research

JoVE Journal

Behavior

Feeding Experimentation Device (FED): Construction and Validation of an Open-source Device for Measuring Food Intake in Rodents

Use of the Operant Orofacial Pain Assessment Device (OPAD) to Measure Changes in Nociceptive Behavior

Use of the Operant Orofacial Pain Assessment Device OPAD to Measure Changes in Nociceptive Behavior

Gebruik van de operante Orofacial Pain Assessment Device (OPAD) om veranderingen in Nociceptieve Gedrag Meet

We present an operant  system for the detection of pain in awake, conscious rodents. The Orofacial  Pain Assessment Device (OPAD) assesses pain behaviors ...

A Procedure to Study the Effect of Prolonged Food Restriction on Heroin Seeking in Abstinent Rats

Een procedure om het effect van langdurig Eten Beperking Heroïne zoek naar in Abstinent Ratten Studie

In human drug addicts, exposure to drug-associated cues or environments that were previously associated with drug taking can trigger relapse during ...

Studying Food Reward and Motivation in Humans

Studeren Eten Beloning en motivatie bij de mens

A key challenge in studying reward processing in humans is to go beyond subjective self-report measures and quantify different aspects of reward such as ...

Assessment of Social Cognition in Non-human Primates Using a Network of Computerized Automated Learning Device (ALDM) Test Systems

Assessment of Social Cognition in Non-human Primates Using a Network of Computerized Automated Learning Device ALDM Test Systems

Evaluatie van Sociale cognitie in niet-menselijke primaten met behulp van een netwerk van geautomatiseerde Automated Learning Device (ALDM) Test Systems

Fagot &#38; Paleressompoulle1 and Fagot &#38; Bonte2 have published an automated learning device (ALDM) for the study of cognitive abilities of monkeys ...

An Unpredictable Chronic Mild Stress Protocol for Instigating Depressive Symptoms, Behavioral Changes and Negative Health Outcomes in Rodents

Een onvoorspelbare chronische milde stress protocol voor Aansporen depressieve symptomen, gedragsveranderingen en Negatieve Health Outcomes in Knaagdieren

Chronic, unresolved stress is a major risk factor for the development of clinical depression. While many preclinical models of stress-induced depression ...

Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila

Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila

Het combineren van kwantitatieve voedselinname-Assays en onder dwang activeren neuronen om te bestuderen van eetlust in Drosophila

Food consumption is under the tight control of the brain, which integrates the physiological status, palatability, and nutritional contents of the food, ...

An Automated T-maze Based Apparatus and Protocol for Analyzing Delay- and Effort-based Decision Making in  Free Moving Rodents

Een geautomatiseerde T-maze op basis van apparatuur en Protocol voor het analyseren van de vertraging en inspanning-gebaseerde besluitvorming in gratis verplaatsen knaagdieren

Many neurological and psychiatric patients demonstrate difficulties and/or deficits in decision making. Rodent models are helpful to produce a deeper ...

A Vibrotactile Feedback Device for Seated Balance Assessment and Training

Een Vibrotactile Feedback apparaat voor zittende evenwicht beoordeling en opleiding

Postural perturbations, motion tracking, and sensory feedback are modern techniques used to challenge, assess, and train upright sitting, respectively. ...

In Vivo Protocol of Controlled Subconcussive Head Impacts for the Validation of Field Study Data

In Vivo Protocol van gecontroleerde Subconcussive hoofd effecten voor de validatie van veldgegevens studie

Subconcussive hits pose a threat to neuronal health as they have shown to induce neuronal structural damage and functional impairment without causing ...

Providing Meaningful Environmental Enrichment and Measuring Saliva Cortisol in Pigs Housed on Slatted Flooring

Het verstrekken van zinvolle milieu verrijking en het meten van speeksel cortisol bij varkens ondergebracht op een roostervloer

As pork is the most consumed meat worldwide, the welfare of animals in the swine industry has increasingly become a major public concern, which imposes a ...