Name: murine full-thickness skin transplantation
Uploaded: 2017-01-02T15:00:00
Description: Watch this Scientific Journal Video about Murine Full-thickness Skin Transplantation at JoVE.com

这项工作是由美国国立卫生研究院资助R01AI077610资助。

所有程序均按照指南的护理与健康研究所（NIH）的实验室动物的用途进行，由约翰霍普金斯大学动物护理和使用委员会（JHUACUC）获得批准。经批准的ACUC协议MO13M292和MO13M370下进行的具体程序。 1.捐赠皮肤丰收<ol><li>麻醉供体小鼠异氟醚（感应蒸发器4％，在维持1 - 2％，通过鼠标锥）。使用脚趾捏撤回反射来监测麻醉深度。 </li><li>使用电动剃须刀，剃动物的背部，并用10％?...

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J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Orthotopic+Hind+Limb+Transplantation+in+the+Mouse.">Orthotopic Hind Limb Transplantation in the Mouse.</a> J Vis Exp. , (2016).</li><li>Oh, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+Novel+Microsurgical+Model+for+Heterotopic,+En+Bloc+Chest+Wall,+Thymus,+and+Heart+Transplantation+in+Mice.">A Novel Microsurgical Model for Heterotopic, En Bloc Chest Wall, Thymus, and Heart Transplantation in Mice.</a> J Vis Exp. , (2016).</li><li>Oberhuber, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Murine+cervical+heart+transplantation+model+using+a+modified+cuff+technique.">Murine cervical heart transplantation model using a modified cuff technique.</a> J Vis Exp. , e50753 (2014).</li><li>Jones, T. 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B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+behaviour+and+fate+of+skin+autografts+and+skin+homografts+in+rabbits:+A+report+to+the+War+Wounds+Committee+of+the+Medical+Research+Council.">The behaviour and fate of skin autografts and skin homografts in rabbits: A report to the War Wounds Committee of the Medical Research Council.</a> J Anat. 78, 176-199 (1944).</li><li>Billingham, R. E., Brent, L., Medawar, P. B., Sparrow, E. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantitative+studies+on+tissue+transplantation+immunity.+I.+The+survival+times+of+skin+homografts+exchanged+between+members+of+different+inbred+strains+of+mice.">Quantitative studies on tissue transplantation immunity. I. The survival times of skin homografts exchanged between members of different inbred strains of mice.</a> Proc R Soc Lond B Biol Sci. 143, 43-58 (1954).</li><li>Garrod, K. R., Cahalan, M. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Murine+skin+transplantation.">Murine skin transplantation.</a> J Vis Exp. , (2008).</li><li>Schmaler, M., Broggi, M. A., Rossi, S. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transplantation+of+tail+skin+to+study+allogeneic+CD4+T+cell+responses+in+mice.">Transplantation of tail skin to study allogeneic CD4 T cell responses in mice.</a> J Vis Exp. , e51724 (2014).</li><li>Bergstresser, P. R., Toews, G. B., Gilliam, J. N., Streilein, J. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Unusual+numbers+and+distribution+of+Langerhans+cells+in+skin+with+unique+immunologic+properties.">Unusual numbers and distribution of Langerhans cells in skin with unique immunologic properties.</a> J Invest Dermatol. 74, 312-314 (1980).</li><li>Chen, H. D., Silvers, W. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Influence+of+Langerhans+cells+on+the+survival+of+H-Y+incompatible+skin+grafts+in+rats.">Influence of Langerhans cells on the survival of H-Y incompatible skin grafts in rats.</a> J Invest Dermatol. 81, 20-23 (1983).</li><li>Chong, A. S., Alegre, M. L., Miller, M. L., Fairchild, R. 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因为由梅达沃，首先在人体研究，然后在兔子和老鼠其介绍，皮肤移植已成为同种异体免疫反应6,7研究的宝贵模型。在这个手稿中，我们提出采用上，下背部皮肤大规模，非血管，皮肤全层移植的典范。各种替代方法，包括使用尾部皮肤或鼠标作为接枝组织来源的耳部皮肤，已经报道了迄今8,9。这些模型展示技术简单的独特优势，并允许高容量的吞吐量。表皮朗格汉斯细胞和真皮...

器官移植是选择用于患者的终末期器官衰竭的治疗和结果都与在外科手术和免疫抑制剂的协议的进步显着提高。然而，长期免疫抑制与显著的副作用，以及促进容忍新的战略发展仍然现代移植研究的目标。 许多动物模型已经开发了用于移植的基础研究，研究移植排斥反应的机制和测试用于防止移植排斥和促进长期耐受1-3的免疫抑制方法。小鼠模型已成为免疫学研究的中流砥柱，由于诊断和治疗性抗体和良好定义的近交系和转基因品系的独家和广阔的可用性。皮肤移植的是，不需要特殊的显微技术和可以简单的程序轻松术后监测。总之，小鼠皮肤移植一直是一个特殊的工具来研究许多方面参与同种免疫反应，包括抗原呈递细胞贩运，和移植排斥4,5期间组织破坏。 在这里，我们展示了一步一步的程序使用鼠标模型皮肤全层移植，而我们描述必要的移植皮肤移植成功的重要步骤。

<table border="0" cellpadding="0" cellspacing="0" width="923">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:247px;">Straight micro forceps&#160;</td>
			<td style="width:125px;">Sigma</td>
			<td style="width:136px;">F4017</td>
			<td style="width:415px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:247px;">Curved micro forceps&#160;</td>
			<td style="width:125px;">Aesculap</td>
			<td style="width:136px;">BD333R</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:247px;">Curved Stevens tenotomy scissors&#160;</td>
			<td style="width:125px;">Aesculap</td>
			<td style="width:136px;">BC905R</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:247px;">Mayo dissecting&#160; scissors</td>
			<td style="width:125px;">Sigma</td>
			<td style="width:136px;">S3146</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:247px;">Micro needle holder</td>
			<td style="width:125px;">Aesculap</td>
			<td style="width:136px;">BM563R</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:247px;">Sterile gauze</td>
			<td style="width:125px;">Covidien</td>
			<td style="width:136px;">441218</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:247px;">6-0 Nylon suture&#160;</td>
			<td style="width:125px;">MWI</td>
			<td style="width:136px;">31849</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:247px;">Plastic Strips Band-Aid</td>
			<td style="width:125px;">Johnson &#38; Johnson</td>
			<td style="width:136px;"></td>
			<td>Obtained from pharmacy</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:247px;">10 cm Petri dish</td>
			<td style="width:125px;">Fisherbrand</td>
			<td style="width:136px;">FB0875712</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:247px;">PBS</td>
			<td style="width:125px;">Quality Biological</td>
			<td style="width:136px;">119069131</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:247px;">Buprenorphine</td>
			<td style="width:125px;"></td>
			<td style="width:136px;"></td>
			<td>DEA Number required; Obtained from hospital pharmacy</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:247px;">Enrofloxacin</td>
			<td style="width:125px;">Bayer Health Care</td>
			<td style="width:136px;">186599</td>
			<td></td>
		</tr>
	</tbody>
</table>

murine full-thickness skin transplantation

鼠全层皮肤移植是一种行之有效的体内模型来研究同种免疫反应和移植排斥。尽管它的应用有限给人类，小鼠皮肤移植已被广泛用于移植研究。该程序是很容易学习和执行，并且它不需要细腻的显微外科技术，也没有广泛的培训。此外，在该模型中移植排斥反应发生在一个非常可重复的免疫反应，并且容易地通过直接检查和触诊监测。此外，与供体匹配的或第三方皮肤移植继发的皮肤移植可以在更复杂的移植模型作为替代，并评估供体特异性耐受简单的方法进行。的并发症是低，并且在一般限于在手术后麻醉过量或呼吸窘迫。移植失败，另一方面，通常发生作为制备较差克的结果船尾，不正确的定位于移植床上，或包扎不当位置。在这篇文章中，我们提出了小鼠全层皮肤移植的协议，并描述必需的成功过程中的重要步骤。

收件人鼠标绷带的位置是该过程的一个重要步骤。皮肤移植物被定位在接受者躯干，肩膀，臀部和脊柱（ 图1）之间。绷带与折叠纱布和两个塑料粘性绷带的组合制成。收件人鼠标向下放置与接枝在纱布上的绷带的中心。使用两个弯曲微钳，绷带的下端首先拉出，然后将绷带的顶部被缠绕在鼠标腹部。 在完全不匹?...

Watch this Scientific Journal Video about Murine Full-thickness Skin Transplantation at JoVE.com

Murine Full-thickness Skin Transplantation

鼠全层皮肤移植是一种行之有效的模型来研究排斥同种免疫的设置。在这里，我们提供了执行的BALB / c每个步骤的教程 - &gt;的C57BL / 6的全层皮肤移植。

鼠全层皮肤移植

Murine full-thickness skin transplantation is a well-established in vivo model to study alloimmune response and graft rejection. Despite its limited ...

Research

JoVE Journal

Immunology and Infection

Murine Model of CD40-activation of B cells

激活的B细胞CD40的小鼠模型

Research on B cells has shown that CD40 activation improves their antigen presentation capacity. When stimulated with interleukin-4 and CD40 ligand ...

科研

免疫与感染

Granulocyte-dependent Autoantibody-induced Skin Blistering

粒细胞相关的自身抗体引起的皮肤起泡

Autoimmune phenomena occur in healthy individuals, but when self-tolerance fails, the autoimmune response may result in specific pathology. According to ...

Transplantation of Tail Skin to Study Allogeneic CD4 T Cell Responses in Mice

尾部皮肤来研究异基因CD4 + T细胞应答的小鼠移植

The study of T cell responses and their consequences during allo-antigen recognition requires a model that enables one to distinguish between donor and ...

Isolation and Transplantation of Different Aged Murine Thymic Grafts.

隔离和不同的老年小鼠胸腺移植的移植。

The mechanisms that regulate the efficacy of thymic selection remain ill-defined. The method presented here allows in vivo analyses of the development and ...

A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Gu&#233;rin

A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-GuÃƒÆ’Ã†'Ãƒâ€šÃ‚Â©rin

基于CFSE-试验鼠皮肤树突状细胞的迁移习进引流淋巴结在与感染<em&gt;牛分枝杆菌</em&gt;卡介苗

Dendritic cells (DCs) are important for initiating immune responses, in part through their ability to acquire and shuttle antigen to the draining lymph ...

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

体外和活体评估移植受体的 T、B 和髓细胞抑制活性和体液反应

The main concern in transplantation is to achieve specific tolerance through induction of regulatory cells. The understanding of tolerance mechanisms ...

Using Robotic Systems to Process and Embed Colonic Murine Samples for Histological Analyses

The understanding of human diseases has been greatly expanded thanks to the study of animal models. Nonetheless, histopathological evaluation of ...

Renal Subcapsular Transplantation of 2'-Deoxyguanosine-Treated Murine Embryonic Thymus in Nude Mice

2'-脱氧核苷在裸体小鼠中治疗鼠胚胎胸腺的肾下囊移植

The thymus is an important central immune organ, which plays an essential role in the development and differentiation of T cells. Thymus transplantation ...

Isolation of Lamina Propria Mononuclear Cells from Murine Colon Using Collagenase E

使用胶原酶E从毛里结肠分离拉米纳普里亚单核细胞

The intestine is the home to the largest number of immune cells in the body. The small and large intestinal immune systems police exposure to exogenous ...

Optimization of the Cuff Technique for Murine Heart Transplantation

穆林心脏移植袖口技术的优化

Murine cardiac transplantation has been performed for more than 40 years. With advancements in microsurgery, certain new techniques have been used to ...