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DOI :
10.3791/55119-v
•
9:30 min
January 18th, 2017
Chapters
0:05
Title
1:03
Open Fluorescence Lifetime Imaging (FLIM)–High Content Analysis (HCA) Plugin Setup
1:51
Instrument Response Function (IRF) Image and Reference Dye Data Acquisition
4:32
Multiwell Plate Sample FLIM Data Acquisition
6:29
Results: Representative FLIM Analyses in Cos and HeLa Cells
8:05
Conclusion
我们目前使用的测定使用荧光共振能量转移(FRET)的读数蛋白质相互作用自动荧光寿命成像(FLIM)一个开源的高内涵分析(HCA)的仪器。此openFLIM-HCA仪数据采集是通过软件写入μManager和数据分析在FLIMfit进行控制。
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