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DOI :
10.3791/55146-v
•
8:34 min
February 22nd, 2017
Chapters
0:05
Title
1:18
Cultivate L. pneumophila and Prepare and Quantify L. pneumophila Outer Membrane Vesicles (OMVs)
3:42
Pre-treat, Infect Macrophages, and Assess Bacterial Replication with a Colony-Forming Unit (CFU) Assay
5:31
Results: Pro-inflammatory Response of Macrophages from L. pneumophila Infection
7:04
Conclusion
这里,我们描述嗜肺军团菌 ( 嗜肺军团菌 )从液体培养外膜囊泡(OMV的)的纯化。然后将这些纯化的囊泡用于巨噬细胞的治疗,以分析其促炎潜力。
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