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DOI :
10.3791/55150-v
•
11:30 min
January 20th, 2017
Chapters
0:05
Title
0:48
Collecting Root and Leaf Samples from Infected Apple Trees
2:22
Yeast 2 Hybrid Screen
7:47
Analysis of Clones from Selective Plates
8:50
Results: Yeast 2 Hybrid to Identify Binding Partners of Bacterial Effector Proteins
9:57
Conclusion
细菌效应蛋白是用于建立成功的感染很重要的。这个协议描述在其天然植物宿主细菌效应蛋白的蛋白质结合配偶的实验鉴定。识别通过酵母双杂交屏幕这些效应的相互作用已成为解开分子致病性策略的一个重要工具。
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