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JoVE Journal

Immunology and Infection

This content is Open Access.

Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen

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11:30 min

January 20th, 2017

January 20th, 2017

11,285 Views

0:05

Title

0:48

Collecting Root and Leaf Samples from Infected Apple Trees

2:22

Yeast 2 Hybrid Screen

7:47

Analysis of Clones from Selective Plates

8:50

Results: Yeast 2 Hybrid to Identify Binding Partners of Bacterial Effector Proteins

9:57

Conclusion

Transcript

Bacterial effector proteins are important for establishing successful infections. This protocol describes the experimental identification of proteinaceous binding partners of a bacterial effector protein in its natural plant host. Identifying these effector interactions via yeast two-hybrid screens has become an important tool in unravelling molecular pathogenicity strategies.

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