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DOI :
10.3791/55153-v
January 24th, 2017
Chapters
0:05
Title
0:38
Stable Transfection of HEK-293 Cells
3:59
Large Scale Antibody Production from Batch Overgrowth Culture
5:12
Antibody Purification by Affinity Chromatograpy Using an Automated Fast Protein Liquid Chromatography System
7:02
Results: Laboratory-scale Production and Purification of Antibody
8:50
Conclusion
这个协议描述在哺乳动物表达系统中生产的治疗性抗体的。所描述的方法包括制备载体DNA,稳定转染和人胚肾293细胞系的无血清适应的,设置大规模培养和纯化使用亲和层析组成。
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