Cloning Genes of Interest in the Multigene Expression System Vector, pMGX
4:11
Insert Gene 2 into the pMGX Vector Containing Gene 1, pMGX-yfg1
7:08
Results: Multigene Expression in pMGX
9:10
Conclusion
Transcript
The overall goal of this procedure is to construct multigene synthetic operons under the control of an inducible T7 RNA polymerase for expressing multiple proteins simultaneously. This method can help researchers in synthetic biology and biochemis
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Cette étude décrit des méthodes pour la co-expression médiée par T7 de plusieurs gènes à partir d'un seul plasmide dans Escherichia coli en utilisant le système de plasmide pMGX.