Name: murine echocardiography of left atrium, aorta, and pulmonary artery
Uploaded: 2017-02-20T15:00:00
Description: Watch this Scientific Journal Video about Murine Echocardiography of Left Atrium, Aorta, and Pulmonary Artery at JoVE.com

This work was supported by the Huffington Center on Aging at Baylor College of Medicine, Geriatrics, CVS DeBakey Heart Center at Houston Methodist Hospital and BCM, NIH RO-1 HL 13870 to ML Entman, as well as a Career Development Award # IK2BX002410 from the United States Department of Veterans Affairs (LMP), Biomedical Laboratory Research and Development Program.
We are immensely grateful to Mark Entman M.D. for sharing his wisdom and supporting this work.

所有的动物都按照健康指南全国学院的照顾和实验动物使用的贝勒医学院的指导方针照顾。 1.建立<ol><li>打开超声心动图系统。从下拉菜单中选择显示“心脏测量”启动软件并点击“初始化”。 </li><li>首先在屏幕的上半部分的“新建”按钮，一项新的研究点击进入学习鼠标的人口统计信息。例如鼠标ID，性别，出生日期，以及操作人员做研究的名字。 </li><li>通过点击“TEMP O...

<ol>
	<li>Upadhya, B., Taffet, G. E., Ping, C., Kitzman, D. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Heart+failure+with+preserved+ejection+fraction+in+the+elderly:+scope+of+the+problem.">Heart failure with preserved ejection fraction in the elderly: scope of the problem.</a> Journal of Molecular and Cellular Cardiology. 83, 73-87 (2015).</li><li>Mclaughlin, V. V., Archer, S. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=ACCF+/+AHA+Expert+Consensus+Document+Expert+Consensus+Document+ACCF+/+AHA+2009+Expert+Consensus+Document+on+Pulmonary+Hypertension+A+Report+of+the+American+College+of+Cardiology+Foundation+Task+Force+on+Expert+Consensus+Documents+and+the+American+Heart+Association.">ACCF / AHA Expert Consensus Document Expert Consensus Document ACCF / AHA 2009 Expert Consensus Document on Pulmonary Hypertension A Report of the American College of Cardiology Foundation Task Force on Expert Consensus Documents and the American Heart Association.</a> Circulation. , 2250-2294 (2009).</li><li>Cieslik, K. A., Taffet, G. E., Carlson, S., Hermosillo, J., Trial, J., Entman, M. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Immune-inflammatory+dysregulation+modulates+the+incidence+of+progressive+fibrosis+and+diastolic+stiffness+in+the+aging+heart.">Immune-inflammatory dysregulation modulates the incidence of progressive fibrosis and diastolic stiffness in the aging heart.</a> Journal of Molecular and Cellular Cardiology. 50 (1), 248-256 (2011).</li><li>Tate, C. A., Taffet, G. E., Hudson, E. K., Blaylock, S. L., McBride, R. P., Michael, L. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Enhanced+calcium+uptake+of+cardiac+sarcoplasmic+reticulum+in+exercise-trained+old+rats.">Enhanced calcium uptake of cardiac sarcoplasmic reticulum in exercise-trained old rats.</a> Am J Physiol Heart Circ Physiol. 258 (2), H431-H435 (1990).</li><li>Kitzman, D. W., Daniel, K. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Diastolic+Heart+Failure+in+the+Elderly.">Diastolic Heart Failure in the Elderly.</a> Heart Failure Clinics. 3 (4), 437-453 (2007).</li><li>Sanderson, J. E., Rusconi, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=How+to+diagnose+diastolic+heart+failure+a+consensus+statement+on+the+diagnosis+of+heart+failure+with+normal+left+ventricular+ejection+fraction+by+the+Heart+Failure+and+Echocardiography+Associations+of+the+European+Society+of+Cardiology.">How to diagnose diastolic heart failure a consensus statement on the diagnosis of heart failure with normal left ventricular ejection fraction by the Heart Failure and Echocardiography Associations of the European Society of Cardiology.</a> Eur Heart J. , 2539-2550 (2007).</li><li>Medrano, G., Hermosillo-Rodriguez, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Left+Atrial+Volume+and+Pulmonary+Artery+Diameter+Are+Noninvasive+Measures+of+Age-Related+Diastolic+Dysfunction+in+Mice.">Left Atrial Volume and Pulmonary Artery Diameter Are Noninvasive Measures of Age-Related Diastolic Dysfunction in Mice.</a> The Journals of Gerontology Series A: Biological Sciences and Medical Sciences. (16), 1-10 (2015).</li><li>Franco, F., Thomas, G. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Magnetic+Resonance+Imaging+and+Invasive+Evaluation+of+Development+of+Heart+Failure+in+Transgenic+Mice+With+Myocardial+Expression+of+Tumor+Necrosis+Factor-+.">Magnetic Resonance Imaging and Invasive Evaluation of Development of Heart Failure in Transgenic Mice With Myocardial Expression of Tumor Necrosis Factor- .</a> Circulation. 99, 448-454 (1999).</li><li>Jenkins, C., Bricknell, K., Marwick, T. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Use+of+real-time+three-dimensional+echocardiography+to+measure+left+atrial+volume:+Comparison+with+other+echocardiographic+techniques.">Use of real-time three-dimensional echocardiography to measure left atrial volume: Comparison with other echocardiographic techniques.</a> Journal of the American Society of Echocardiography. 18 (9), 991-997 (2005).</li><li>Ujino, K., Barnes, M. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Two-dimensional+echocardiographic+methods+for+assessment+of+left+atrial+volume.">Two-dimensional echocardiographic methods for assessment of left atrial volume.</a> The American journal of cardiology. 98 (9), 1185-1188 (2006).</li><li>Scalia, G. M., Scalia, I. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=ePLAR+-+The+echocardiographic+Pulmonary+to+Left+Atrial+Ratio+-+A+novel+non-invasive+parameter+to+differentiate+pre-capillary+and+post-capillary+pulmonary+hypertension.">ePLAR - The echocardiographic Pulmonary to Left Atrial Ratio - A novel non-invasive parameter to differentiate pre-capillary and post-capillary pulmonary hypertension.</a> International Journal of Cardiology. 212, 379-386 (2016).</li></ol>

有成功测量LAV，主动脉和PA直径的三个关键步骤。在设定了它完全删除在胸前的毛皮是非常重要的;如果不这样做会导致图像质量的干扰。在图像采集步骤，重要的是获得具有其相应的探针和过滤器各图像每秒帧从探头变化来探测：即25兆赫和心脏筛选为LAV的评估和30兆赫和评估腹部过滤的主动脉和PA的直径。研究者有时由图像获取差如果鼠标具有的脂肪量很大，或者如果一个肋使得难以得到良好的?...

目前的方法教调查员如何衡量和使用LAV，主动脉直径和PA直径二维超声心动图小鼠下异氟醚。心脏衰竭与老年人射血分数（HFPEF）影响的那些80年高达10％及以上1和结果显著发病率和死亡率。显著的死亡率也由肺动脉高压（PH），一个阴险的疾病过程有类似症状HFPEF，其中肺动脉压力升高导致劳力性呼吸困难，进行正确的心脏衰竭，并经常提出的死亡发生。 2既HFPEF和PH值的日益流行表示用于开发了一种方法，允许在非侵入性，非终端的方法准确的评价，并在小鼠模型干预监测。 通过心室艺术改变老化导致舒张功能恶化erial硬化，血管功能障碍，炎症3，受损的钙调节4，降低β肾上腺素能响应性和身体功能失调产生积极的减缓放松和增加被动刚度。随着时间的推移这将导致增加的LV充盈压和洛杉矶的代偿性肿大。 五 虽然其他病因，如瓣膜功能不全（二尖瓣关闭不全或狭窄）和浸润工艺导致在LA 1压力和容积升高，欧洲心脏病学会支持添加LA大小为LV功能的无创性反映。 6 洛杉矶量和舒张功能的微创措施之间的相关性进行了研究，小鼠。在功能差异相关洛杉矶量确定侵入年龄组为14位和31个月之久的米冰。在14个月大的老鼠，LA体积舒张功能的三个标准侵入措施相关-dP / DTMIN（R 2 = 0.5，P &lt;0.05），头（松弛时间常数（R 2 =。6， p &lt;0.05），左室舒张末压（R 2 = 0.25，p &lt;0.05）; 31个月大的老鼠，洛杉矶量和-dP / DTMIN（R 2 = 0.92，p之间的关系&lt; 0.05），左室舒张末压（R 2 = 0.61，p &lt;0.05），但明显与头的关系是不太清楚。因此，洛杉矶量不仅跨组，但年龄组中舒张功能障碍增加。7 在使用导管技术来评估心脏功能，虽然严格而可靠的，被限制的鼠模型的心脏功能的研究由于其与反复评估不相容性。 8替代侵入的措施，如MRI和三维超声心动图也可以是比2D超声心动图技术更准确，但它们比较昂贵; 2D超声心动图被认为足以洛杉矶量评估。 9，10 对LA体积和PA直径与超声心动图评估允许产生在肺动脉阻力主要增加导致与在洛杉矶的压力或LAV从那些没有变化的增加的PA直径，其中肺动脉和左心房都放大作为模型之间的区别对心脏的左侧充盈压升高的结果。这种方法是采取斯卡利亚等。谁发现，在人，超声心动图肺动脉左心房比（ePLAR）是给患者预先肺毛细血管高血压和capillaryhypertension柱之间精确区分的参数。 11

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murine echocardiography of left atrium, aorta, and pulmonary artery

本方法教导研究者如何测量并使用LAV通过超声心动图中左心室舒张压慢性升高的替代，以及获得小鼠主动脉和PA直径的测量。 比的年龄10天老年小鼠中可以使用本技术进行分析。该技术是由3个主要步骤：设置，图像采集和图像分析。的设立步骤包括得到含有1％异氟烷麻醉小鼠，剃须它，并在仰卧位置到加热的EKG板，其中所述图像采集将于胶带它的。图像获取步骤包括学习以识别心脏结构和获得所有与它的相应探针和轴所需的图像，以便能够计算体积和直径。图像分析步骤包括在计算机软件的帮助下测定以前获得的图像。

对LA体积和PA直径与超声心动图评估允许其中增加PA的直径没有变化的LAV从那些肺动脉和左心房扩大都在充盈压升高的两个结果增加肺动脉阻力结果模型之间的区别左侧的心脏。显示在两个病理生理学差异图像示于图4。洛杉矶和PA的图像从年轻，缺氧引起肺动脉高压和年龄有关的左心室舒张功能障碍。肺动脉高压模型的发展离不开左心房容积超过4周周期的变化?...

Watch this Scientific Journal Video about Murine Echocardiography of Left Atrium, Aorta, and Pulmonary Artery at JoVE.com

Murine Echocardiography of Left Atrium, Aorta, and Pulmonary Artery

以下方案描述了用于获得左心房容积（LAV），主动脉（AO）的直径，以及肺动脉（PA）的直径在小鼠心脏超声图像的获取和分析的方法。这种技术是一种非侵入性的，非终端程序，允许心肺功能的评估。

左心房，主动脉和肺动脉的超声心动图小鼠

The present methodology teaches the investigator how to measure and use the LAV as a surrogate of chronic elevations in Left Ventricular diastolic ...

Research

JoVE Journal

Medicine

Murine Echocardiography and Ultrasound Imaging

小鼠超声心动图和超声成像

Rodent models of cardiac pathophysiology represent a valuable research tool to investigate mechanism of disease as well as test new therapeutics.1  ...

科研

医学

Murine Fetal Echocardiography

小鼠胎儿超声心动图

Transgenic mice displaying abnormalities in  cardiac development and function represent a powerful tool for the understanding  the molecular mechanisms ...

Assessment of Right Ventricular Structure and Function in Mouse Model of Pulmonary Artery Constriction by Transthoracic Echocardiography

右心室结构和功能的肺动脉缩窄经胸超声小鼠模型的评估

Emerging clinical data support the notion that RV dysfunction is critical to the pathogenesis of cardiovascular disease and heart failure1-3. Moreover, ...

A Murine Model of Myocardial Ischemia-reperfusion Injury through Ligation of the Left Anterior Descending Artery

心肌缺血再灌注损伤的通过左前降支结扎小鼠模型

Acute or chronic myocardial infarction (MI) are cardiovascular events resulting in high morbidity and mortality. Establishing the pathological mechanisms ...

Murine Left Anterior Descending (LAD) Coronary Artery Ligation: An Improved and Simplified Model for Myocardial Infarction

Murine Left Anterior Descending LAD Coronary Artery Ligation: An Improved and Simplified Model for Myocardial Infarction

鼠左前降支（LAD）冠状动脉结扎：改进和简化模型的心肌梗死

Ischemic heart disease (IHD), or acute coronary syndrome (ACS), is one of the leading causes of death in the United States. IHD is characterized by ...

Direct Re-implantation of Left Coronary Artery into the Aorta in Adults with Anomalous Origin of Left Coronary Artery from the Pulmonary Artery (ALCAPA)

Direct Re-implantation of Left Coronary Artery into the Aorta in Adults with Anomalous Origin of Left Coronary Artery from the Pulmonary Artery ALCAPA

左冠状动脉直接再注入与从肺动脉左冠状动脉起源异常成人主动脉（ALCAPA）

Anomalous origin of the left coronary artery from the pulmonary artery (ALCAPA) is a rare congenital anomaly which is one of leading causes of myocardial ...

Left Atrial Stenosis Induced Pulmonary Venous Arterialization and Group 2 Pulmonary Hypertension in Rat

左心房狭窄致大鼠肺静脉动脉化及第2组肺动脉高压

The mechanism of mitral stenosis-induced pulmonary venous arterialization and group 2 pulmonary hypertension (PH) is unclear. There is no rodent model of ...

Murine Myocardial Infarction Model using Permanent Ligation of Left Anterior Descending Coronary Artery

使用左前部降冠动脉的永久结扎的月心肌梗死模型

Myocardial infarction (MI) and acute coronary diseases are among the most prominent causes of death in population with western lifestyle. The murine ...

Use of a Percutaneous Ventricular Assist Device/Left Atrium to Femoral Artery Bypass System for Cardiogenic Shock

使用经皮心室辅助装置/左心房至股动脉旁路系统治疗心源性休克

The left atrial to femoral artery bypass (LAFAB) system is a mechanical circulatory support (MCS) device used in cardiogenic shock (CS) that bypasses the ...

Left Coronary Artery Ligation: A Surgical Murine Model of Myocardial Infarction

左冠状动脉结扎术：心肌梗死的外科小鼠模型

Ischemic heart disease and subsequent myocardial infarction (MI) is one of the leading causes of mortality in the United States and around the world. In ...