Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/55239-v
June 12th, 2017
Chapters
0:05
Title
1:02
Primer Design for PCR Amplification of Gene Flanks
10:23
Conclusion
8:53
Results: The OSCAR Method Generates a Gene Deletion Plasmid for Fungi in a Single Reaction
2:49
Production of OSCAR Constructs
5:48
Agrobacterium tumefaciens Mediated Transformation (ATMT) of Fungi
7:45
Deletion Mutant Identification by PCR
通过同源重组产生的基因缺失突变体是基因功能研究的黄金标准。描述了用于快速产生缺失构建体的OSCAR(农杆菌 - 重组 - 质粒的一步构建)方法。 土壤杆菌介导的真菌转化如下。最后,提出了基于真菌转化体基因缺失的基于PCR的确认方法。
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved