0:46
Generation and Purification of PCR Products Corresponding to Two Mutated and Partially Overlapping Histone Gene Fragments
2:06
Fusion PCR of the Two Partially Overlapping Fragments to Generate Full Size PCR Products for Integration
3:20
Co-transformation, Screen for 5-FOA-resistant Transformants, Purification of 5-FOA-resistant Colonies, and Loss of Backbone Plasmid
5:11
Results: In Situ Mutagenesis of HHT2 in Yeast