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DOI :
10.3791/55267-v
•
9:37 min
March 5th, 2017
Chapters
0:05
Title
0:52
Generation of iCas9 hPSC Lines
4:15
gRNA Transfection of iCas9 hPSCs
6:00
In Vitro hPSC Differentiation into Glucose-responsive Pancreatic β Cells
7:48
Results: Efficient Genetic Modification in hPSCs Using the iCRISPR Platform
8:50
Conclusion
协议来生成使用iCRISPR平台HPSC突变系和分化hPSCs成葡萄糖反应β样细胞中有描述。结合基因组编辑技术,HPSC-定向分化为血统决定因素在人的发展和疾病发展中的作用的系统分析了强大的平台。
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