Name: minimally invasive transverse aortic constriction in mice
Uploaded: 2017-03-14T15:00:00
Description: Watch this Scientific Journal Video about Minimally Invasive Transverse Aortic Constriction in Mice at JoVE.com

我们要感谢在香港理工大学生命科学（ULS）大学的研究设施，以提供VEVO 2100超声系统。特别感谢温家宝纯一博士从生物医学工程，香港理工大学，跨学科司提供训练和便利。这项工作是由香港政府拨款计划GRF 17127215，764812和HKU6 / CRF / 11G比利KC周的支持。

该协议遵循了委员会对教学与研究（CULATR）使用活的动物在香港大学的指导方针。 1.操作场地准备<ol><li>消毒用75％异丙醇的手术部位。调节加热垫温度至37℃，以避免在心脏和变化受试动物的呼吸率。 </li><li>使用在重力位移消毒灭菌的外科器械在121℃进行30分钟，或通过其它适当的方法。 </li><li>取2 - 4 30号针头和针保持器曲线它们，如在图1看到的那样，以使对...

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	<li>Rockman, H. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Segregation+of+atrial-specific+and+inducible+expression+of+an+atrial+natriuretic+factor+transgene+in+an+in+vivo+murine+model+of+cardiac+hypertrophy.">Segregation of atrial-specific and inducible expression of an atrial natriuretic factor transgene in an in vivo murine model of cardiac hypertrophy.</a> Proc Natl Acad Sci U S A. 88 (18), 8277-8281 (1991).</li><li>de Almeida, A. C., van Oort, R. J., Wehrens, X. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transverse+aortic+constriction+in+mice.">Transverse aortic constriction in mice.</a> J Vis Exp. (38), (2010).</li><li>Hu, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Minimally+invasive+aortic+banding+in+mice:+effects+of+altered+cardiomyocyte+insulin+signaling+during+pressure+overload.">Minimally invasive aortic banding in mice: effects of altered cardiomyocyte insulin signaling during pressure overload.</a> Am J Physiol Heart Circ Physiol. 285 (3), H1261-H1269 (2003).</li><li>Shimizu, I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Excessive+cardiac+insulin+signaling+exacerbates+systolic+dysfunction+induced+by+pressure+overload+in+rodents.">Excessive cardiac insulin signaling exacerbates systolic dysfunction induced by pressure overload in rodents.</a> J Clin Invest. 120 (5), 1506-1514 (2010).</li><li>Tarnavski, O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+surgical+models+in+cardiovascular+research.">Mouse surgical models in cardiovascular research.</a> Methods Mol Biol. 573, 115-137 (2009).</li><li>Zhang, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+modified+murine+model+for+the+study+of+reverse+cardiac+remodelling.">A modified murine model for the study of reverse cardiac remodelling.</a> Exp Clin Cardiol. 18 (2), e115-e117 (2013).</li><li>Hartley, C. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Doppler+estimation+of+reduced+coronary+flow+reserve+in+mice+with+pressure+overload+cardiac+hypertrophy.">Doppler estimation of reduced coronary flow reserve in mice with pressure overload cardiac hypertrophy.</a> Ultrasound Med Biol. 34 (6), 892-901 (2008).</li><li>Scherrer-Crosbie, M., Thibault, H. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Echocardiography+in+translational+research:+of+mice+and+men.">Echocardiography in translational research: of mice and men.</a> J Am Soc Echocardiogr. 21 (10), 1083-1092 (2008).</li><li>Faerber, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induction+of+heart+failure+by+minimally+invasive+aortic+constriction+in+mice:+reduced+peroxisome+proliferator-activated+receptor+gamma+coactivator+levels+and+mitochondrial+dysfunction.">Induction of heart failure by minimally invasive aortic constriction in mice: reduced peroxisome proliferator-activated receptor gamma coactivator levels and mitochondrial dysfunction.</a> J Thorac Cardiovasc Surg. 141 (2), 492-500 (2011).</li><li>Li, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assessment+of+Cardiac+Morphological+and+Functional+Changes+in+Mouse+Model+of+Transverse+Aortic+Constriction+by+Echocardiographic+Imaging.">Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging.</a> J Vis Exp. (112), (2016).</li></ol>

洛克人和同事发明了第一个方法主动脉缩窄为学习压力超负荷的心脏的方法。这使研究开始揭露高血压和心脏衰竭1的细胞和分子的后果。一种改进的方法，微创横向主动脉缩窄（MTAC），提供了左心室压力负荷和心脏衰竭的诱导的顺从，依赖于时间的方法，没有以往的横向主动脉缩窄9的缺点。该过程本身可以比TAC被显著更容易且具有增加的存活率进行。该方法?...

<html>这篇文章的总体目标是演示如何横向小鼠主动脉缩窄（TAC）可以被修改以产生一个更简单，更方便的手术。 TAC最早在1991年1引入并启用分子生物学家研究导致肥大在左心室和心脏衰竭，特别是在小动物与操纵基因组2，3，4，5，6中的细胞和分子通路。尽管它在产生所需的压力超负荷功效，该技术最终由难以执行的程序和由它的低存活率的限制。一位外科医生在进行TAC必须能够执行气管插管，开胸，为了防止肺萎陷1使用人工通气。 内容“&gt;通常，一个简单的和更小侵入性方法是期望的。因此，微创横向主动脉缩窄（MTAC）被制定3，虽然结果（具体地说，左心室肥大）是一样的，该过程涉及显著较少装置和向动物损伤较小。MTAC证明是具有更大的生存率，90％以上，理想的方法，并且具有唯一的15至25分钟。成功的手术后死亡的罕见情况下的操作时间可以归因于太主动脉弓-tight收缩，造成了巨大的左心室超负荷和致命减少血液供应的关键器官如肾脏。 在这个步骤完成后，超声心动图可以用来评估带和心脏形态和功能的成功。的高频超声波系统可被用于测量直径，并在左，右流的变化颈动脉，以显现主动脉弓缩窄为峰值流速的测量，并确定左心室壁的厚度和功能参数7,8。安乐死后，器官也可以收集对用苏木精和曙红染色和天狼猩红胶原染色的组织病理学研究，后者为纤维化的观测是特别有用的。

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			<td>1 inch 30 Gauge Needles</td>
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			<td>Curved as in the procedure and Figure 1</td>
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			<td>27 Gauge Needle</td>
			<td>BD, Franklin Lakes, NJ 07417, USA.&#160;</td>
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			<td>Make blunt and smooth for spacer</td>
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			<td>6/0 Dafilon</td>
			<td>B. Braun, 34212 Melsungen, Hessen, Germany.</td>
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			<td>6/0 Silkam</td>
			<td>B. Braun, 34212 Melsungen, Hessen, Germany.</td>
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			<td>Sterile gloves</td>
			<td>A.R. Medicom, Inc (Asia), Hong Kong.</td>
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			<td>Mannings, Quarry Bay, Hong Kong.</td>
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			<td>Curved Iris Forcep</td>
			<td>FST, British Columbia V7H 0A6, Canada.</td>
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			<td>Micro Olsen-Hegar Needle Holder</td>
			<td>WPI, Sarasota, FL 34240, USA.&#160;</td>
			<td>501989</td>
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			<td>Stereo Micrioscope</td>
			<td>WPI, Sarasota, FL 34240, USA.&#160;</td>
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			<td>GenTeal Gel</td>
			<td>Novartis, East Hanover, NJ 07936, USA.&#160;</td>
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			<td>Kent Scientific Corporation, Torrington, CT 06790, USA.&#160;</td>
			<td>DCT-20</td>
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minimally invasive transverse aortic constriction in mice

微创横向主动脉缩窄（MTAC）为横向主动脉的小鼠比标准开胸横向主动脉缩窄（TAC）缩颈更理想的方法。虽然横向主动脉缩窄为左心室高压的感应一个高功能的方法，它是一个更加困难和冗长过程由于其使用人工通气的气管插管。 TAC是常常也少生存，作为较新的方法，MTAC，既不要求肋骨和肋间肌也没有气管插管与通气设置切割。在MTAC，相对于一个开胸访问到胸腔，通过设在前壁颈部正中切口到达主动脉弓。甲状腺拉回以显示胸骨切迹。胸骨随后降低到第二肋水平，并通过分离的结缔组织和胸腺到达主动脉弓简单。从还有，缝合线能够围绕拱形缠绕并与间隔并列，然后胸骨切口和皮肤可以关闭。 MTAC是更快和更小侵入性的方式来诱导左心室高血压，并使用于高通量研究的可能性。缩颈的成功可以采用高频经胸超声心动图，尤其是彩色多普勒和脉冲波多普勒，确定主动脉弓的流速进行验证和左，右颈动脉，血管的尺寸，并左心室功能和形态。一个成功的收缩也会触发显著病理变化，如间质及血管周围纤维化心肌细胞肥大。这里，MTAC的程序中描述，显示了如何在颈动脉所得流量的变化可以用超声心动图，大体形态，并在心脏组织病理学变化进行检查。

MTAC使得能够显著高血压在左心室及时的诱导。 如在图1看到的，主要工具的程序编制简单和最小的，仅需要2 - 4 30号针头以用聚氨酯管子被弯曲并保持在适当位置。手术过程本身也不太复杂，因为相比TAC，并且可以容易地进行。一旦甲状腺已收回，胸骨被切断，胸腺被移动以清除视图。主动脉弓是用于结扎过程（ ...

Watch this Scientific Journal Video about Minimally Invasive Transverse Aortic Constriction in Mice at JoVE.com

Minimally Invasive Transverse Aortic Constriction in Mice

微创横向主动脉缩窄（MTAC）节省常规横向主动脉缩窄（TAC）的要领，同时消除了气管插管使用呼吸机。它被证明是对左心室过载，特别是在翻译研究高通量研究一个非常理想的方法。

微创横向主动脉缩窄小鼠

Minimally invasive transverse aortic constriction (MTAC) is a more desirable method for the constriction of the transverse aorta in mice than standard ...

Research

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In vitro Measurements of Tracheal Constriction Using Mice

In vitro Measurements of Tracheal Constriction Using Mice

在利用老鼠的气管收缩的体外测量

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科研

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Ascending Aortic Constriction in Rats for Creation of Pressure Overload Cardiac Hypertrophy Model

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Ascending aortic constriction is the most common and successful surgical model for creating pressure overload induced cardiac hypertrophy and heart ...

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Orthotopic bladder cancer xenografts are the gold standard to study molecular cellular manipulations and new therapeutic agents&#160;in vivo. Suitable ...

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在横向主动脉缩窄的小鼠模型和心脏形态学评估功能改变通过超声心动图成像

Transverse aortic constriction (TAC) in mice has been used as a valuable model to study mechanisms of cardiac hypertrophy and heart failure1. A reliable ...

Technique of Minimally Invasive Transverse Aortic Constriction in Mice for Induction of Left Ventricular Hypertrophy

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Transverse aortic constriction (TAC) in mice is one of the most commonly used surgical techniques for experimental investigation of pressure ...

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Bone healing models are necessary to analyze the complex mechanisms of fracture healing to improve clinical fracture treatment. During the last decade, an ...

A Closed-chest Model to Induce Transverse Aortic Constriction in Mice

诱导小鼠横向主动脉狭窄的闭合胸模型

Research on cardiac hypertrophy and heart failure is frequently based on pressure overload mouse models induced by TAC. The standard procedure is to ...

Electromagnetic Navigation Transthoracic Nodule Localization for Minimally Invasive Thoracic Surgery

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重建显微外科缺血再灌注损伤研究的临床前大鼠模型

Ischemia-reperfusion injury is the main cause of flap failure in reconstructive microsurgery. The rat is the preferred preclinical animal model in many ...

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Capnography is commonly used to monitor patient&#8217;s ventilatory status. While sidestream capnography has been shown to provide a reliable assessment ...