Name: the rabbit model of accelerated atherosclerosis: a methodological perspective of the iliac artery balloon injury
Uploaded: 2017-10-03T12:30:00
Description: Watch this Scientific Journal Video about The Rabbit Model of Accelerated Atherosclerosis: A Methodological Perspective of the Iliac Artery Balloon Injury at JoVE.com

この作品は、スイスの国立科学財団助成 150271 によって支えられました。

、スイス連邦獣医局、スイス連邦共和国 (FR 2015/58)、フリブール州の獣医のオフィスによって承認されたこの実験的プロトコル。
 注: 3.2 2.8 キロの間の重量を量る雄 NZW ウサギが使用されました。動物は、従来の条件 (12 h 光と暗いサイクル、アドリブの水と食糧を提供) の下で収容されました。バルーン侵食前に動物は 1 週間中に、彼らは通常の食事の飼料で飼育した順応...

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	<li>Mozaffarian, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Heart+disease+and+stroke+statistics--2015+update:+a+report+from+the+American+Heart+Association.">Heart disease and stroke statistics--2015 update: a report from the American Heart Association.</a> Circulation. 131, e29-e322 (2015).</li><li>Boone, L. R., Brooks, P. A., Niesen, M. I., Ness, G. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mechanism+of+resistance+to+dietary+cholesterol.">Mechanism of resistance to dietary cholesterol.</a> J Lipids. 2011, 101242 (2011).</li><li>Kapourchali, F. 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ウサギ腸骨動脈のアテローム性動脈硬化モデルは動脈硬化研究で広く使用されます。このプロトコルでウサギは急速にコレステロールの食事療法のみで開発された自発的な病変と比較してより厳しいと高度なプラクを開発しました。重要なは、動物は手術から迅速に回復します。
粥状動脈硬化のための主要な刺激は、血管内皮細胞を傷つける、distends 容器壁<sup class="xref"...

動脈硬化性プラークの破裂は、先進国1死の主要な原因の 1 つです。過去十年にわたって研究は、プラークの進展に関与するいくつかの分子・細胞メカニズムを展開してきた、病気の進行の複雑なメカニズムを解明するだけでなく、新しいテストにも努力が必要まだ続けている治療近づきます。動脈硬化症を研究するいくつかの動物モデルを提案されています。遺伝子操作、コレステロール供給または機械的内皮損傷動脈硬化マウス、ウサギ、ミニブタなどのほとんどの動物モデルで共有標準戦略であります。これらのうち、NZW ウサギは、正常ラットおよびマウスでは、食餌療法のコレステロール2,3,4は大幅吸収しない中コレステロールの食事療法に敏感です。ウサギは自発的にコレステロール豊富な食事療法5,6にうんざりしたいくつかの線維性のコンポーネントとマクロファージの豊富な大動脈病変を開発します。しかし、動脈硬化 plaquesby コレステロール食事療法だけで6,7を給餌を誘発する 4-8 ヶ月の長い準備時間は実験的設定のほとんどのための主な欠点です。比較的短時間で病変を誘導するための追求、Baumgarter、ステューダー8でコレス トロールが高い食事療法およびバルーン傷害の組み合わせを開発しました。この手法の全体的な目標は、高コレステロール血症ウサギにおける泡沫細胞 (人間の脂肪のストリークに類似) 2 週間以内で構成される動脈硬化性プラークを誘導するためです。本手法では、nzw 種高コレステロール血症ウサギの腸骨動脈に高度なバルーンカテーテルを用いた Baumgarter の法に基づく動脈壁損傷の手順について説明します。
コレステロール豊富な食事と一緒に解消の内皮化が誘起されるバルーン傷害、動脈硬化に します。バルーン傷害はアテローム性動脈硬化病変の形成を加速し、均一なサイズと分布のプラークが生成されます。内膜肥厚は傷害の後の数日以内時間と内膜細胞浸潤の開始の期間にわたって増加します。実質的なマクロファージと脂肪筋はバルーン傷害の 7 ~ 10 日後現われ始めるし、アメリカ心臓協会分類 II 型病変として表されます。ウサギ バルーン傷害は、しばしば大動脈プラークの組成を研究する実行されます。新生内膜内皮細胞間接着分子の高レベルを表しています。プラークは、内側と外の変化に関連付けられます。動脈硬化病変、脂質、増殖平滑筋細胞 (SMCs)、コラーゲン線維と再生内皮の下で蓄積、大抵タイプ II 本質的に炎症性細胞の構成されます。原則としてひと大動脈9,10で報告に類似していたウサギのプラクの位相分布、大動脈腸骨動脈と比較してサイズは大きくより大きい長さのプラークになります。しかし、動脈硬化家兎のサイトとして総腸骨動脈を使用しての主な利点は、アクセシビリティ、ひと冠動脈11、制服病変開発12、高組織率に筋肉のコンテンツの類似性活動13と一貫性のある容器寸法ひと冠動脈形態と血管造影のエンドポイントに商業的に製造されたデバイスの評価に匹敵します。生きている動物のウサギ腸骨動脈のプラークを分析する侵襲的、非侵襲的な方法を検討しました。磁気共鳴画像 (MRI) をさらに 2.35 テスラ MR システム14の助けを借りての使用については以前のレポート、血管内超音波 (IVUS) や光コヒーレンス断層法 (OCT) カテーテルを適切にすることができます画像に適用ウサギ腸骨動脈で動脈硬化プラーク。総腸骨動脈は、高解像度の超音波を使用して超音波検査バリアフリーと大動脈は、この手法で調べることができます。
過去 10 年間でさらにプラーク進行15とプラーク回帰16のメカニズムを理解するバルーン傷害のこの家兎モデルをしました。さらに、モデルは、スタチン系薬剤、標準的な抗血小板剤、抗酸化剤17,18エベロリムスなどの薬剤溶出ステントなどの新規治療薬の影響を研究に使用されていますかゾタロリムス溶出ステント19,20新生内膜肥厚。このモデルは、血管内カテーテル21近赤外蛍光イメージングを調査する使用されています。

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>New Zealand White rabbits</td>
			<td>Charles River laboratories,France</td>
			<td>Cre:KBL(NZW)</td>
			<td></td>
		</tr>
		<tr>
			<td>Cholesterol rich diet</td>
			<td>Ssniff spezialdi&#228;ten</td>
			<td>Ssniff EF K High Fat and Cholesterol</td>
			<td></td>
		</tr>
		<tr>
			<td>Glass bead sterilizer-Germinator 500</td>
			<td>VWR, Leicestershire, UK</td>
			<td>101326-488</td>
			<td></td>
		</tr>
		<tr>
			<td>Fogarty balloon embolectomy catheters, 2 French</td>
			<td>Edwards Lifesciences, Switzerland</td>
			<td>120602F</td>
			<td>For single use only</td>
		</tr>
		<tr>
			<td>Luer Lock Syringe</td>
			<td>Becton, Dickinson and Company, USA</td>
			<td>309628</td>
			<td></td>
		</tr>
		<tr>
			<td>Thermopad Type 226</td>
			<td>Solis, Switzerland AG</td>
			<td>397387</td>
			<td></td>
		</tr>
		<tr>
			<td>Buprenorphine- Temgesic</td>
			<td>Reckitt Benckiser AG, Switzerland</td>
			<td>7.68042E+12</td>
			<td></td>
		</tr>
		<tr>
			<td>Isoflurane</td>
			<td>Piramal Critical Care, Inc, Bethlehem, PA 18017</td>
			<td>2667-46-7</td>
			<td></td>
		</tr>
		<tr>
			<td>Anaesthesia machine-combi-vet Base Anesthesia System</td>
			<td>Rothacher Medical GmbH, Switzerland</td>
			<td>CV 30-301-A</td>
			<td></td>
		</tr>
		<tr>
			<td>Cardell touch veterinary vital signs monitor</td>
			<td>Midmark, Ohio, USA</td>
			<td>8013-001</td>
			<td></td>
		</tr>
		<tr>
			<td>Ophthalmic ointment-Humigel</td>
			<td>Virbac, France</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Animal hair clippers</td>
			<td>Aesculap AG, Germany</td>
			<td>GT420</td>
			<td></td>
		</tr>
		<tr>
			<td>Disinfectant-Betadine solution</td>
			<td>MundipharmaMedicalCompany, Switzerland</td>
			<td>14671-1203</td>
			<td></td>
		</tr>
		<tr>
			<td>Dumont #7 Forceps</td>
			<td>FST Germany</td>
			<td>11274-20</td>
			<td></td>
		</tr>
		<tr>
			<td>Medium and small microscissors</td>
			<td>Medline International Switzerland S&#224;rl</td>
			<td>UC4337</td>
			<td></td>
		</tr>
		<tr>
			<td>Microvascular clamps</td>
			<td>FST, Germany</td>
			<td>18051-28</td>
			<td></td>
		</tr>
		<tr>
			<td>Papaverine</td>
			<td>ESCA chemicals, Switzerland</td>
			<td>RE 356 803</td>
			<td></td>
		</tr>
		<tr>
			<td>Vein Pick</td>
			<td>Harvard Apparatus, Cambridge, UK</td>
			<td>72-4169</td>
			<td>For single use only</td>
		</tr>
		<tr>
			<td>Saline</td>
			<td>Laboratorium Dr. G. Bichsel AG, , Switzerland</td>
			<td>1330055</td>
			<td></td>
		</tr>
		<tr>
			<td>Polysorb 5-0 suture</td>
			<td>Covidien AG, Switzerland</td>
			<td>UL 202</td>
			<td>Monofilament</td>
		</tr>
		<tr>
			<td>Sulfadoxine and Trimethoprim-Trimethazol</td>
			<td>Werner Stricker AG, Switzerland</td>
			<td>Swissmedic Nr. 50&#39;361</td>
			<td></td>
		</tr>
		<tr>
			<td>Antiseptic- Octenisept</td>
			<td>Sch&#252;lke &#38; Mayr AG, Switzerland</td>
			<td>GTIN: 4032651214068</td>
			<td></td>
		</tr>
		<tr>
			<td>Phosphate Buffered Saline</td>
			<td>Roth</td>
			<td>1058.1</td>
			<td></td>
		</tr>
		<tr>
			<td>Isobutanol-2-Methylbutane</td>
			<td>Sigma-Aldrich, Switzerland</td>
			<td>M32631-1L</td>
			<td></td>
		</tr>
		<tr>
			<td>Optimum Cutting Temperature compound-Tissue-Tek</td>
			<td>VWR Chemicals, Belgium</td>
			<td>25608-930</td>
			<td></td>
		</tr>
		<tr>
			<td>Cryostat</td>
			<td>Leica, Glattbrugg, Switzerland</td>
			<td>Leica CM1860 UV</td>
			<td></td>
		</tr>
		<tr>
			<td>Glass slide- Superfrost Plus</td>
			<td>Thermo Scientific</td>
			<td>4951PLUS4</td>
			<td></td>
		</tr>
		<tr>
			<td>Mayer&#39;s Haematoxylin</td>
			<td>Sigma-Aldrich, Switzerland</td>
			<td>MHS32-1L</td>
			<td></td>
		</tr>
		<tr>
			<td>Eosin 0.5% aq.</td>
			<td>Sigma-Aldrich, Switzerland</td>
			<td>HT110232-1L</td>
			<td></td>
		</tr>
		<tr>
			<td>Oil Red O</td>
			<td>Sigma-Aldrich, Switzerland</td>
			<td>O0625-25G</td>
			<td></td>
		</tr>
		<tr>
			<td>&#945;-smooth muscle actin antibody</td>
			<td>Abcam, UK.</td>
			<td>ab7817</td>
			<td></td>
		</tr>
		<tr>
			<td>Macrophage Clone RAM11 antibody</td>
			<td>DAKO, Switzerland</td>
			<td>M063301</td>
			<td></td>
		</tr>
		<tr>
			<td>Hoechst</td>
			<td>Abcam, UK.</td>
			<td>ab145596</td>
			<td></td>
		</tr>
		<tr>
			<td>Goat polyclonal Secondary Antibody (Chromeo 546)</td>
			<td>Abcam, UK.</td>
			<td>ab60316</td>
			<td></td>
		</tr>
		<tr>
			<td>Alexa Fluor 488/547</td>
			<td>Abcam, UK.</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Glycergel Mounting Medium, Aqueous</td>
			<td>DAKO, Switzerland</td>
			<td>C056330</td>
			<td></td>
		</tr>
		<tr>
			<td>Hematoxylin for Movat pentachrome staining</td>
			<td>Sigma-Aldrich, Switzerland</td>
			<td>H3136-25G</td>
			<td></td>
		</tr>
		<tr>
			<td>Ferric chloride for Movat pentachrome staining</td>
			<td>Sigma-Aldrich, Switzerland</td>
			<td>157740-100G</td>
			<td></td>
		</tr>
		<tr>
			<td>Iodine for Movat staining</td>
			<td>Sigma-Aldrich, Switzerland</td>
			<td>207772-100G</td>
			<td></td>
		</tr>
		<tr>
			<td>Potassium iodide for Movat pentachrome staining</td>
			<td>Sigma-Aldrich, Switzerland</td>
			<td>60400-100G-F</td>
			<td></td>
		</tr>
		<tr>
			<td>Alcian blue for Movat staining</td>
			<td>Sigma-Aldrich, Switzerland</td>
			<td>A5268-10G</td>
			<td></td>
		</tr>
		<tr>
			<td>Strong Ammonia for Movat pentachrome staining</td>
			<td>Sigma-Aldrich, Switzerland</td>
			<td>320145-500ML</td>
			<td></td>
		</tr>
		<tr>
			<td>Brilliant crocein MOO for Movat pentachrome staining</td>
			<td>Sigma-Aldrich, Switzerland</td>
			<td>210757-50G</td>
			<td></td>
		</tr>
		<tr>
			<td>Acid Fuchsin for Movat pentachrome staining</td>
			<td>Sigma-Aldrich, Switzerland</td>
			<td>F8129-50G</td>
			<td></td>
		</tr>
		<tr>
			<td>Sodium Thiosulfate for Movat pentachrome staining</td>
			<td>Sigma-Aldrich, Switzerland</td>
			<td>72049-250G,</td>
			<td></td>
		</tr>
		<tr>
			<td>Phosphotungstic acid for Movat pentachrome staining</td>
			<td>Sigma-Aldrich, Switzerland</td>
			<td>79690-100G</td>
			<td></td>
		</tr>
		<tr>
			<td>Crocin for Movat pentachrome staining</td>
			<td>Sigma-Aldrich, Switzerland</td>
			<td>17304-5G</td>
			<td></td>
		</tr>
		<tr>
			<td>EUKITT for Movat pentachrome staining</td>
			<td>Sigma-Aldrich, Switzerland</td>
			<td>03989-100ML</td>
			<td></td>
		</tr>
	</tbody>
</table>

the rabbit model of accelerated atherosclerosis: a methodological perspective of the iliac artery balloon injury

動脈硬化性プラークの開発と破壊に続く冠動脈閉塞による急性冠症候群は、先進世界での死の主要な原因です。ニュージーランド ホワイト (nzw 種) ウサギは、アテローム性動脈硬化の研究の動物モデルとして広く使用されます。彼らは動脈硬化食事にうんざりした自発的な病変を開発します。ただし、これは 4-8 ヶ月の長い時間を必要があります。さらに強化し、粥状動脈硬化を促進、動脈硬化の食事と機械的血管内皮傷害の組み合わせがしばしば利用されます。家兎動脈硬化性プラークを誘導するため提示された手順は、動脈硬化の飼料 NZW ウサギの左総腸骨動脈の内皮細胞を破壊するのにバルーンカテーテルを使用します。バルーンカテーテルによるこのような機械的な損傷は、時間依存的新生内膜脂質の蓄積を開始する炎症反応の連鎖を誘発します。次のバルーン傷害ショー新生内膜肥厚広範な脂肪浸潤、高平滑筋細胞内容マクロファージの存在と動脈硬化性プラークには、泡沫細胞が派生しました。この手法、シンプルで再現性のある、内腸骨動脈内長の制御のプラークを生成します。全体の手順は 20-30 分以内に完了します。手順は、安全低死亡率では、実質的な内膜肥厚病変に高い成功を提供しています。バルーンカテーテルのプロシージャは、2 週間以内動脈硬化症における動脈傷害の結果を誘発しました。このモデルは病の病理を調査するため使用することができます画像診断と新しい治療戦略を評価します。

総腸骨動脈のバルーン傷害は、(図 1) の合併症もなく正常に実行されました。20 〜 30 分の怪我のため手術時間の合計は、のみ 1 つの腸骨動脈、および両方の動脈の損傷のための 35 〜 45 分を行います。ウサギはバルーン損傷後 1 h 以内に回復。すべての動物は、有意な体重を失うことがなく健康な登場。感染症、浮腫や動脈血栓症発生しませんで...

Watch this Scientific Journal Video about The Rabbit Model of Accelerated Atherosclerosis: A Methodological Perspective of the Iliac Artery Balloon Injury at JoVE.com

The Rabbit Model of Accelerated Atherosclerosis: A Methodological Perspective of the Iliac Artery Balloon Injury

動脈硬化症の動物モデルは、メカニズムを理解してプラークの開発や破断、先進国の死亡原因を防止するための新しいアプローチを調査するために不可欠です。このプロトコルは、ウサギ腸骨動脈の動脈硬化性プラークを誘発するのにバルーン傷害と高コレステロール食の組み合わせを使用します。

加速動脈硬化家兎モデル: 内腸骨動脈バルーン傷害の方法論的視点

Acute coronary syndrome resulting from coronary occlusion following atherosclerotic plaque development and rupture is the leading cause of death in the ...

Research

JoVE Journal

Medicine

A Murine Model of Myocardial Ischemia-reperfusion Injury through Ligation of the Left Anterior Descending Artery

左前下の動脈の結紮を通して心筋虚血再灌流傷害のマウスモデル

Acute or chronic myocardial infarction (MI) are cardiovascular events resulting in high morbidity and mortality. Establishing the pathological mechanisms ...

Vascular Balloon Injury and Intraluminal Administration in Rat Carotid Artery

ラット頸動脈における血管バルーン傷害と管腔内投与

The carotid artery balloon injury model in rats has been well established for over two decades. It remains an important method to study the molecular and ...

A Rat Carotid Balloon Injury Model to Test Anti-vascular Remodeling Therapeutics

ラット頸動脈バルーン損傷モデルは、抗血管リモデリングの治療薬をテストします

The rat carotid balloon injury is a well-established surgical model that has been used to study arterial remodeling and vascular cell proliferation. It is ...

Induction of Accelerated Atherosclerosis in Mice: The "Wire-Injury" Model

マウスにおける加速アテローム性動脈硬化症の誘導:ワイヤー傷害モデル

Atherosclerosis is a proliferative fibro-inflammatory disease developing in the arterial wall, inducing a deficient blood flow or a lack of blood flow. ...

Balloon-based Injury to Induce Myointimal Hyperplasia in the Mouse Abdominal Aorta

マウスの腹部大動脈 Myointimal 過形成を誘導するために気球を利用した傷害

The use of animal models is essential for a better understanding of MH, one major cause for arterial stenosis.In this article, we demonstrate a murine ...

In Vivo Gene Transfer to the Rabbit Common Carotid Artery Endothelium

In Vivo Gene Transfer to the Rabbit Common Carotid Artery Endothelium

生体内でウサギの一般的な頚動脈内皮細胞への遺伝子導入

The goal of this method is to introduce a transgene into the endothelium of isolated segments of both rabbit common carotid arteries. The method achieves ...

An Experimental Model of Diet-Induced Metabolic Syndrome in Rabbit: Methodological Considerations, Development, and Assessment

ウサギの食餌によるメタボリック シンドロームの実験的モデル: 方法論的検討・開発・評価

In recent years, obesity and metabolic syndrome (MetS) have become a growing problem for public health and clinical practice, given their increased ...

Ferric Chloride-induced Canine Carotid Artery Thrombosis: A Large Animal Model of Vascular Injury

塩化第二鉄によるイヌ総頚動脈血栓症: 血管損傷の大動物モデル

Occlusive arterial thrombosis leading to cerebral ischemic stroke and myocardial infarction contributes to ~13 million deaths every year globally. Here, ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

ワイヤー図法を使用してマウスの腸間膜動脈の等尺性収縮力測定

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...

A Rat Carotid Artery Pressure-Controlled Segmental Balloon Injury with Periadventitial Therapeutic Application

過期治療アプリケーションを用いてラット頸動脈圧制御区分バルーン損傷

Cardiovascular disease remains the leading cause of death and disability worldwide, in part due to atherosclerosis. Atherosclerotic plaque narrows the ...