Name: evaluating the effect of environmental chemicals on honey bee development from the individual to colony level
Uploaded: 2017-04-01T14:00:00
Description: Watch this Scientific Journal Video about Evaluating the Effect of Environmental Chemicals on Honey Bee Development from the Individual to Colony Level at JoVE.com

Denne forskning blev støttet af Grant 105AS-13.2.3-BQ-B1 fra Bureau of Animal og Plantesundhed Inspection and Quarantine, Rådet for landbrug, Executive Yuan og Grant 103-2313-B-197-002-MY3 fra ministeriet for Videnskab og Teknologi (MOST).

1. Præparater <ol><li> Lav 1 liter 50% sukkersirup. Opløs 1 kg saccharose i 1 I Hedeselskabet 2 O. </li><li> Forberede pyriproxyfen (PPN) opløsning i BLD. Gøre 1,1 I 10.000 ppm PPN stamopløsning og fortyndes 100 ml PPN opløsning i 1 liter steriliseret Hedeselskabet 2 O. Opbevares ved 4 ° C. </li><li> Fortynde PPN stamopløsningen til slutkoncentrationer på 0,1, 1, 10 og 100 mg / kg (ppm) i BLD for det følgende eksperiment. </li><li> Make PPN-sirup (for kolonien niveau). Fortynde PPN lage...

<ol>
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W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Impaired+olfactory+associative+behavior+of+honeybee+workers+due+to+contamination+of+imidacloprid+in+the+larval+stage.">Impaired olfactory associative behavior of honeybee workers due to contamination of imidacloprid in the larval stage.</a> PLoS One. 7, e49472 (2012).</li><li>Urlacher, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Measurements+of+chlorpyrifos+levels+in+forager+bees+and+comparison+with+levels+that+disrupt+honey+bee+odor-mediated+learning+under+laboratory+conditions.">Measurements of chlorpyrifos levels in forager bees and comparison with levels that disrupt honey bee odor-mediated learning under laboratory conditions.</a> J. Chem. Ecol. 42 (2), 127-138 (2016).</li><li>Chen, Y. W., Wu, P. S., Yang, E. C., Nai, Y. S., Huang, Z. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+impact+of+pyriproxyfen+on+the+development+of+honey+bee+(Apis+mellifera+L.)+colony+in+field.">The impact of pyriproxyfen on the development of honey bee (Apis mellifera L.) colony in field.</a> J. Asia Pac. Entomol. 19 (3), 589-594 (2016).</li><li>Dennehy, T. J., DrGain, B. A., Harpold, V. S., Brink, S. A., Nichols, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Whitefly Resistance to Insecticides in Arizona: 2002 and 2003 Results. , 1926-1938 (2004).</li><li>Yang, E. C., Wu, P. S., Chang, H. C., Chen, Y. 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L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+dietary+transgenic+Bt+corn+pollen+on+larvae+of+Apis+mellifera+and+Galleria+mellonella.">Effects of dietary transgenic Bt corn pollen on larvae of Apis mellifera and Galleria mellonella.</a> J. Apicult.Res. 42 (4), 77-81 (2003).</li><li>Kaftanoglu, O., Linksvayer, T. A., Page, R. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rearing+honey+bees,+apis+mellifera,+in+vitro+1,+effects+of+sugar+concentrations+on+survival+and+development.">Rearing honey bees, apis mellifera, in vitro 1, effects of sugar concentrations on survival and development.</a> J. Insect Sci. 11 (96), 1-10 (2011).</li><li>Vandenberg, J. 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Dronningen-begrænset æglægning metode og dronning-udveksling metode er kritiske trin til opsætning af honningbi grupper til feltforsøg inden for denne protokol. Dronningen-begrænset æglægning metode tillader synkronisering af livscyklus honningbier. Derfor kan forskerne vælge 1 dag-gamle larver af samme alder for behandling med forskellige doser af pesticider. For queen-udveksling metode blev dronningen udveksles mellem del A (4 rammer) og B (5 billeder) for at opnå forskellige udviklingsstadier i honningbi fo...

Tilstedeværelsen af pesticider i miljøet er en af de mest alvorlige problemer, som påvirker livet for en honningbi 1, 2, 3. Flere undersøgelser har påvist den fælles tilstedeværelse af pesticidrester i honning bikolonier og biprodukter. I Taiwan, den gennemsnitlige anvendelse af pesticider var 11-12 kg / ha hvert år (fra 2005 til 2013). Mængden af pesticider, der anvendes i Taiwan er højere end i EU-landene, og de latinamerikanske lande 4, 5. Med andre ord, er fra stadet miljø lider alvorlig pesticid stress, især i Taiwan og muligvis i andre lande. Honningbien Apis mellifera er en af de store bestøvere i landbrugssystemer 6 og det producerer også værdifulde produkter såsom honning. Men honningbier er udstillingered til forskellige pesticider og disse pesticider kan bringes tilbage til bistader efter fouragere på blomster, der er blevet sprøjtet med pesticider, når de indsamler nektar og pollen 7, 8. De kan også blive udsat for pesticider ved biavlerne selv har til formål at kontrollere problemer med skadedyr inde bistader 9, 10, 11. Fordi honningbi larver fodret med sygeplejerske bier for deres udvikling, larver, droner og endda dronningen kan udsættes for disse pesticider-kontamineret nektar og pollen 12. Toksiciteten af forskellige pesticider til honningbier skal løses 13. Der er gjort mange forsøg på at vurdere spørgsmålene om miljømæssige pesticidrester. Yang et al. testet påvirkningen af ​​det neurotoksiske insektmidlet imidacloprid på udviklingen af ​​honningbi larver ibikube og rapporterede, at en sub-letal dosis af imidacloprid resulterede i olfaktoriske associative opførsel af de voksne bier 14. Også, Urlacher et al. undersøgte de subletale virkninger af et organophosphat pesticider, chlorpyrifos, på en honningbi arbejdstagers læring ydeevne under laboratorieforhold 15. I vores tidligere undersøgelse, vi evaluerede effekten af et insekt vækst regulator (IGR), pyriproxyfen (PPN), på larver honningbier 16. I dette papir, vi præsenterer metoder til evaluering af de kemiske virkninger på udviklingen af ​​honningbier. Honey bee fodringsmetoder blev beskrevet og anvendt til enten individuelle honningbier eller til en koloni. Først testede vi forskellige koncentrationer af pesticid-forurenet basisk larvediæt (BLD) på larver i kolonierne at vurdere virkningen af pesticidet på individuelle honningbier in vivo. Vi fortsatte derefter med at simulere den naturlige conditioner af pesticidet ved anvendelse pesticid-forurenet sirup i bistader. Ved denne fremgangsmåde vil PPN, som er meget udbredt mod skadedyr 17 og er skadeligt for udviklingen af honningbi-larver og pupper 16, 18, 19, være en indikator til at repræsentere den negative virkning af pesticidet i marken.

<table border="0" cellpadding="0" cellspacing="0" width="887">
	<tbody>
		<tr height="22">
			<td height="22" style="height:22px;width:193px;">Honey bee box</td>
			<td style="width:243px;">SAN-YI Honey Factory</td>
			<td style="width:155px;">W1266</td>
			<td style="width:297px;">Honeybees rearing</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:193px;">Queen excluder (between frames)</td>
			<td style="width:243px;">SAN-YI Honey Factory</td>
			<td style="width:155px;">I1575</td>
			<td style="width:297px;">Queen limitation&#160;</td>
		</tr>
		<tr height="23">
			<td height="23" style="height:23px;width:193px;">Queen excluder (on top )</td>
			<td style="width:243px;">SAN-YI Honey Factory</td>
			<td style="width:155px;">I1566</td>
			<td style="width:297px;">Queen limitation on top&#160;</td>
		</tr>
		<tr height="23">
			<td height="23" style="height:23px;width:193px;">Bee brush</td>
			<td style="width:243px;">SAN-YI Honey Factory, Taiwan</td>
			<td>W1414</td>
			<td style="width:297px;">clean the bees on frame gently</td>
		</tr>
		<tr height="18">
			<td height="18" style="height:18px;width:193px;">Bee feeder</td>
			<td style="width:243px;">SAN-YI Honey Factory, Taiwan</td>
			<td>P0219</td>
			<td style="width:297px;">feed sugar syrup to colony</td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;">Transparent slide</td>
			<td style="width:243px;">Wan-Shih-Chei, Taiwan (http://www.mbsc.com.tw/a01goods.asp?s_id=40)</td>
			<td>1139</td>
			<td style="width:297px;">Mark the larval area on the frames (Material: Polyethylene Terephthalate, PET) (Size= Length*Width*thick= 29.7mm*21mm*0.1mm)</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:193px;">24 well tissu culture plate</td>
			<td style="width:243px;">Guangzhou Jet Bio-Filtration Co., Ltd</td>
			<td style="width:155px;">TCP011024</td>
			<td style="width:297px;">Rearing pupae from extraction</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:193px;">Autoclave</td>
			<td style="width:243px;">Tomin medical equipmenco., LTD.</td>
			<td style="width:155px;">TM-321</td>
			<td style="width:297px;">Make sterilized distilled deionized water (ddH2O)</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:193px;">P20 pipetman</td>
			<td style="width:243px;">Gilson</td>
			<td style="width:155px;">F123600</td>
			<td style="width:297px;">Add PPN into bee larval food pool</td>
		</tr>
		<tr height="25">
			<td height="25" style="height:25px;width:193px;">Incubator&#160;</td>
			<td style="width:243px;">Yihder Co., Ltd.</td>
			<td style="width:155px;">LE-550RD</td>
			<td style="width:297px;">Rearing pupae from extraction</td>
		</tr>
		<tr height="23">
			<td height="23" style="height:23px;width:193px;">Kimwipes</td>
			<td style="width:243px;">COW LUNG INSTRUMENT CO., LTD</td>
			<td style="width:155px;">KCS34155</td>
			<td style="width:297px;">Rearing pupae from extraction</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:193px;">Royal jelly</td>
			<td style="width:243px;">National Ilan University (NIU)</td>
			<td style="width:155px;">NIU</td>
			<td style="width:297px;">Make basic larval diet (BLD)</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:193px;">D-(+)-Glucose</td>
			<td style="width:243px;">Sigma</td>
			<td style="width:155px;">G8270</td>
			<td style="width:297px;">Make basic larval diet (BLD)</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:193px;">D-(-)-Fructose</td>
			<td style="width:243px;">Sigma</td>
			<td style="width:155px;">F0127</td>
			<td style="width:297px;">Make basic larval diet (BLD)</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:193px;">Yeast extract</td>
			<td style="width:243px;">CONDA, pronadisa</td>
			<td style="width:155px;">1702</td>
			<td style="width:297px;">Make basic larval diet (BLD)</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:193px;">Sucrose</td>
			<td style="width:243px;">Taiwan sugar coporation</td>
			<td style="width:155px;">E01071010</td>
			<td style="width:297px;">Make sugar syrup for bee food</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:193px;">Pyriproxyfen (11%)</td>
			<td style="width:243px;">LIH-NUNG CHEMICAL CO.. LTD.</td>
			<td style="width:155px;">Registration No. 1937</td>
			<td style="width:297px;">Insect growth regulator (IGR) used in the experiment</td>
		</tr>
	</tbody>
</table>

evaluating the effect of environmental chemicals on honey bee development from the individual to colony level

The presence of pesticides in the beekeeping environment is one of the most serious problems that impacts the life of a honey bee. Pesticides can be brought back to the beehive after the bees have foraged on flowers that have been sprayed with pesticides. Pesticide contaminated food can be exchanged between workers which then feed larvae and therefore can potentially affect the development of honey bees. Thus, residual pesticides in the environment can become a chronic damaging factor to honey bee populations and gradually lead to colony collapse. In the presented protocol, honey bee feeding methods are described and applied to either an individual honey bee or to a colony. Here, the insect growth regulator (IGR) pyriproxyfen (PPN), which is widely used to control pest insects and is harmful to the development of honey bee larvae and pupae, is used as the pesticide. The presenting procedure can be applied to other potentially harmful chemicals or honeybee pathogens for further studies.

For honningbien felttest blev en dronning begrænset til 4-frame sektion for at lægge æg. Dette trin kan øge kuld tæthed i en ramme og lette efterfølgende observationer. Hver behandling var mærket, og honningbier udvikling klart blev observeret gennem et transparent objektglas. In vivo fodring af PPN-BLD til Honey Bee larver i bistadet blev udført til præcist at vurdere indflydelsen af PPN på udviklingen af honningbier i kolonien. Anvendelse af det in vivo fodr...

Watch this Scientific Journal Video about Evaluating the Effect of Environmental Chemicals on Honey Bee Development from the Individual to Colony Level at JoVE.com

Evaluating the Effect of Environmental Chemicals on Honey Bee Development from the Individual to Colony Level

Heri præsenterer vi en fremgangsmåde til foder pesticid forurenede fødevarer til både en individuel honningbi og en bikube koloni. Proceduren evaluerer pesticidet virkning på individuelle honningbier ved in vivo tilførsel af basisk larvediæt og også på den naturlige tilstand bikube koloni.

Evaluering af effekten af ​​Environmental Chemicals på Honey Bee Udvikling fra den enkelte til Colony Level

The presence of pesticides in the beekeeping environment is one of the most serious problems that impacts the life of a honey bee. Pesticides can be ...

Research

JoVE Journal

Environment

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Automatiseret Gel Size Selection For at forbedre kvaliteten af ​​Next-generation sequencing biblioteker fremstillet fra Environmental Water Prøver

Next-generation sequencing of environmental samples can be challenging because of the variable DNA quantity and quality in these samples. High quality DNA ...

Exploring the Effects of Atmospheric Forcings on Evaporation: Experimental Integration of the Atmospheric Boundary Layer and Shallow Subsurface

Udforskning virkningerne af Atmosfæriske Drivkræfter på Fordampning: Eksperimentel Integration af det atmosfæriske grænselag og Shallow Subsurface

Evaporation is directly influenced by the interactions between the atmosphere, land surface and soil subsurface. This work aims to experimentally study ...

Removal of Trace Elements by Cupric Oxide Nanoparticles from Uranium In Situ Recovery Bleed Water and Its Effect on Cell Viability

Removal of Trace Elements by Cupric Oxide Nanoparticles from Uranium In Situ Recovery Bleed Water and Its Effect on Cell Viability

Fjernelse af Trace Elements af cuprioxid Nanopartikler fra uran<em&gt; In Situ</em&gt; Recovery Bleed Vand og dens effekt på celle levedygtighed

In situ recovery (ISR) is the predominant method of uranium extraction in the United States. During ISR, uranium is leached from an ore body and extracted ...

A Flow-through Exposure System for Evaluating Suspended Sediments Effects on Aquatic Life

En Flow-through Exposure system til evaluering suspenderet sediment Virkninger på vandlevende

This paper describes the Fish Larvae and Egg Exposure System (FLEES). The flow-through exposure system is used to investigate the effects of suspended ...

Extraction of Organochlorine Pesticides from Plastic Pellets and Plastic Type Analysis

Ekstraktion af organiske chlorpesticider fra plastpellets og plasttypeanalyse

Plastic resin pellets, categorized as microplastics (&#8804;5 mm in diameter), are small granules that can be unintentionally released to the environment ...

Preparation of DMMTAV and DMDTAV Using DMAV for Environmental Applications: Synthesis, Purification, and Confirmation

Preparation of DMMTAV and DMDTAV Using DMAV for Environmental Applications: Synthesis, Purification, and Confirmation

Forberedelse af DMMTAV og DMDTAV bruger DMAV for miljømæssige programmer: syntese, rensning og bekræftelse

Dimethylated thioarsenicals such as dimethylmonothioarsinic acid (DMMTAV) and dimethyldithioarsinic acid (DMDTAV), which are produced by the metabolic ...

The Effect of the Application of Thyme Essential Oil on Microbial Load During Meat Drying

Effekten af anvendelsen af timian æteriske olie på mikrobielle belastning under kød tørring

Meat is a high protein meal that is used in the preparation of jerky, a popular food snack, where preservation and safety are important. To assure food ...

Protocol for Acute and Chronic Ecotoxicity Testing of the Turquoise Killifish Nothobranchius furzeri

Protocol for Acute and Chronic Ecotoxicity Testing of the Turquoise Killifish Nothobranchius furzeri

Protokol for akut og kronisk Økotoksicitet afprøvning af turkis Killifish Nothobranchius furzeri

The killifish Nothobranchius furzeri is an emerging model organism in the field of ecotoxicology and its applicability in acute and chronic ecotoxicity ...

Measuring Hypopharyngeal Gland Acinus Size in Honey Bee (Apis mellifera) Workers

Measuring Hypopharyngeal Gland Acinus Size in Honey Bee Apis mellifera Workers

Måling af Hypopharyngeal kirtel Acinus størrelse i honningbi (Apis mellifera) arbejdstagere

The nurse hypopharyngeal glands produce the protein fraction of the worker and royal jelly that is fed to developing larvae and queens. These paired ...

Composition and Distribution Analysis of Bioaerosols Under Different Environmental Conditions

Sammensætning og Distribution analyse af Bioaerosols Under forskellige miljøforhold

Variable microorganisms in particulate matter (PM) under different environmental conditions may have significant impacts on human health. In this study, ...

Evaluering af effekten af ​​Environmental Chemicals på Honey Bee Udvikling fra den enkelte til Colony Level

Evaluering af effekten af Environmental Chemicals på Honey Bee Udvikling fra den enkelte til Colony Level