Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/55304-v
March 16th, 2017
Chapters
0:05
Title
1:53
3D Caco-2 Cell Culture on a Gelatinous Protein Solution
3:23
Immunofluorescence Staining of Epithelial Transporters in 3D Caco-2 Cells
5:12
Measurement of 5-HT Uptake in Mouse Ileum
6:29
Equilibration and Tissue Treatment
7:41
Quantification of the Mucosal to serosal Flux (Jm-s) and 3[H]-5-HT Accumulation
8:42
Results: The Expression and Function of SERT Protein Measured in Caco-2 3D Cell Cultures and Ileal Mucosa Ex Vivo
9:57
Conclusion
우리는 3 차원으로 성장 카코 -2 세포를 시험관 내 세포 배양 모델 및 마우스 창자의 생체 모델을 이용하여 장내 세로토닌 운반체 (SERT) 기능과 발현의 조절을 연구하는 간단한 방법을 설명한다. 이러한 방법은 다른 상피 수송의 연구에 적용 할 수있다.
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved