Name: microfluidic platform with multiplexed electronic detection for spatial tracking of particles
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Description: Watch this Scientific Journal Video about Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles at JoVE.com

This work was supported by National Science Foundation Award No. ECCS 1610995. The authors would like to thank the Institute of Electronics and Nanotechnology and the Parker H. Petit Institute for Bioengineering and Bioscience staff for their support in using shared facilities. The authors also would like to thank Chia-Heng Chu for his help in preparing the manuscript.

Kodlama Elektrotların 1. Tasarım Not: Şekil 1a micropatterned elektrotlar 3-D yapısını göstermektedir. <ol><li> Mikroakışkan kanal 23 kodlama için dört adet 7-bitlik Altın kodları bir dizi tasarlayın. <ol><li> İki doğrusal geri beslemeli kayan-register (LFSRs), ilkel bir polinomu temsil eden her Construct. </li><li> 7-bit m dizileridir bir tercih çifti oluşturmak için LFSRs kullanın. </li><li> Döngüsel m dizil...

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	<li>De Roy, K., Clement, L., Thas, O., Wang, Y., Boon, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Flow+cytometry+for+fast+microbial+community+fingerprinting.">Flow cytometry for fast microbial community fingerprinting.</a> Water Res. 46 (3), 907-919 (2012).</li><li>Vives-Rego, J., Lebaron, P., Nebe-von Caron, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Current+and+future+applications+of+flow+cytometry+in+aquatic+microbiology.">Current and future applications of flow cytometry in aquatic microbiology.</a> FEMS Microbiol Rev. 24 (4), 429-448 (2000).</li><li>Alvarez-Barrientos, A., Arroyo, J., Cant&#243;n, R., Nombela, C., S&#225;nchez-P&#233;rez, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Applications+of+flow+cytometry+to+clinical+microbiology.">Applications of flow cytometry to clinical microbiology.</a> Clin Microbiol Rev. 13 (2), 167-195 (2000).</li><li>Toner, M., Irimia, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Blood-on-a-chip.">Blood-on-a-chip.</a> Annu Rev Biomed Eng. 7, 77-103 (2005).</li><li>Mehling, M., Tay, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microfluidic+cell+culture.">Microfluidic cell culture.</a> Current Opin Biotech. 25, 95-102 (2014).</li><li>Sarioglu, A. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+microfluidic+device+for+label-free,+physical+capture+of+circulating+tumor+cell+clusters.">A microfluidic device for label-free, physical capture of circulating tumor cell clusters.</a> Nat Methods. 12 (7), 685-691 (2015).</li><li>Cermak, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High-throughput+measurement+of+single-cell+growth+rates+using+serial+microfluidic+mass+sensor+arrays.">High-throughput measurement of single-cell growth rates using serial microfluidic mass sensor arrays.</a> Nat Biotechnol. , (2016).</li><li>Gossett, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Label-free+cell+separation+and+sorting+in+microfluidic+systems.">Label-free cell separation and sorting in microfluidic systems.</a> Anal Bioanal Chem. 397 (8), 3249-3267 (2010).</li><li>Tsutsui, H., Ho, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cell+separation+by+non-inertial+force+fields+in+microfluidic+systems.">Cell separation by non-inertial force fields in microfluidic systems.</a> Mech Res Commun. 36 (1), 92-103 (2009).</li><li>Edwards, T. L., Gale, B. K., Frazier, A. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+microfabricated+thermal+field-flow+fractionation+system.">A microfabricated thermal field-flow fractionation system.</a> Anal Chem. 74 (6), 1211-1216 (2002).</li><li>Wang, M. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microfluidic+sorting+of+mammalian+cells+by+optical+force+switching.">Microfluidic sorting of mammalian cells by optical force switching.</a> Nat Biotechnol. 23 (1), 83-87 (2005).</li><li>Shields, C. W., Reyes, C. D., L&#243;pez, G. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microfluidic+cell+sorting:+a+review+of+the+advances+in+the+separation+of+cells+from+debulking+to+rare+cell+isolation.">Microfluidic cell sorting: a review of the advances in the separation of cells from debulking to rare cell isolation.</a> Lab Chip. 15 (5), 1230-1249 (2015).</li><li>Gawad, S., Schild, L., Renaud, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Micromachined+impedance+spectroscopy+flow+cytometer+for+cell+analysis+and+particle+sizing.">Micromachined impedance spectroscopy flow cytometer for cell analysis and particle sizing.</a> Lab Chip. 1 (1), 76-82 (2001).</li><li>Haandb&#230;k, N., B&#252;rgel, S. C., Heer, F., Hierlemann, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterization+of+subcellular+morphology+of+single+yeast+cells+using+high+frequency+microfluidic+impedance+cytometer.">Characterization of subcellular morphology of single yeast cells using high frequency microfluidic impedance cytometer.</a> Lab Chip. 14 (2), 369-377 (2014).</li><li>Bayley, H., Martin, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Resistive-pulse+sensing-from+microbes+to+molecules.">Resistive-pulse sensing-from microbes to molecules.</a> Chem Rev. 100 (7), 2575-2594 (2000).</li><li>Polling, D., Deane, S. C., Burcher, M. R., Glasse, C., Reccius, C. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Coded+electrodes+for+low+signal-noise+ratio+single+cell+detection+in+flow-through+impedance+spectrophy.">Coded electrodes for low signal-noise ratio single cell detection in flow-through impedance spectrophy.</a> , 3-7 (2010).</li><li>Javanmard, M., Davis, R. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Coded+corrugated+microfluidic+sidewalls+for+code+division+multiplexing.">Coded corrugated microfluidic sidewalls for code division multiplexing.</a> IEEE Sensors J. 13 (5), 1399-1400 (2013).</li><li>Balakrishnan, K. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Node-pore+sensing:+a+robust,+high-dynamic+range+method+for+detecting+biological+species.">Node-pore sensing: a robust, high-dynamic range method for detecting biological species.</a> Lab Chip. 13 (7), 1302-1307 (2013).</li><li>Emaminejad, S., Talebi, S., Davis, R. W., Javanmard, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multielectrode+sensing+for+extraction+of+signal+from+noise+in+impedance+cytometry.">Multielectrode sensing for extraction of signal from noise in impedance cytometry.</a> IEEE Sensors J. 15 (5), 2715-2716 (2015).</li><li>Spencer, D., Caselli, F., Bisegna, P., Morgan, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High+accuracy+particle+analysis+using+sheathless+microfluidic+impedance+cytometry.">High accuracy particle analysis using sheathless microfluidic impedance cytometry.</a> Lab Chip. 16 (2016), 2467-2473 (2016).</li><li>Liu, R., Wang, N., Kamili, F., Sarioglu, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microfluidic+CODES:+a+scalable+multiplexed+electronic+sensor+for+orthogonal+detection+of+particles+in+microfluidic+channels.">Microfluidic CODES: a scalable multiplexed electronic sensor for orthogonal detection of particles in microfluidic channels.</a> Lab Chip. 16 (8), 1350-1357 (2016).</li><li>Buehrer, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Code+Division+Multiple+Access+(CDMA).">Code Division Multiple Access (CDMA).</a> Synthesis Lectures on Communications. 1 (1), 1-192 (2006).</li><li>Proakis, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Digital Communications. , (1989).</li><li>Patel, P., Holtzman, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Analysis+of+a+simple+successive+interference+cancellation+scheme+in+a+DS/CDMA+system.">Analysis of a simple successive interference cancellation scheme in a DS/CDMA system.</a> IEEE J Sel Areas Commun. 12 (5), 796-807 (1994).</li><li>Hui, A., Letaief, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Successive+interference+cancellation+for+multiuser+asynchronous+DS/CDMA+detectors+in+multipath+fading+links.">Successive interference cancellation for multiuser asynchronous DS/CDMA detectors in multipath fading links.</a> IEEE Trans Commun. 46 (3), 384-391 (1998).</li><li>Whittle, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prediction+and+regulation+by+linear+least-square+methods.">Prediction and regulation by linear least-square methods.</a> J Macroecon. 7 (1), 126 (1985).</li><li>Whitesides, G., Ostuni, E., Takayama, S., Jiang, X., Ingber, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Soft+lithography+in+biology+and+biochemistry.">Soft lithography in biology and biochemistry.</a> Annu Rev Biomed Eng. 3 (1), 335-373 (2001).</li><li>Zhe, J., Jagtiani, A., Dutta, P., Hu, J., Carletta, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+micromachined+high+throughput+Coulter+counter+for+bioparticle+detection+and+counting.">A micromachined high throughput Coulter counter for bioparticle detection and counting.</a> J Micromech Microeng. 17 (2), 304-313 (2007).</li><li>Song, Y., Yang, J., Pan, X., Li, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High-throughput+and+sensitive+particle+counting+by+a+novel+microfluidic+differential+resistive+pulse+sensor+with+multidetecting+channels+and+a+common+reference+channel.">High-throughput and sensitive particle counting by a novel microfluidic differential resistive pulse sensor with multidetecting channels and a common reference channel.</a> Electrophoresis. 36 (4), 495-501 (2015).</li><li>Watkins, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microfluidic+CD4++and+CD8++T+lymphocyte+counters+for+point-of-care+HIV+diagnostics+using+whole+blood.">Microfluidic CD4+ and CD8+ T lymphocyte counters for point-of-care HIV diagnostics using whole blood.</a> Sci Transl Med. 5 (214), 214ra170 (2013).</li><li>Chen, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Portable+Coulter+counter+with+vertical+through-holes+for+high-throughput+applications.">Portable Coulter counter with vertical through-holes for high-throughput applications.</a> Sensor Actuat B-Chem. 213, 375-381 (2015).</li><li>Jagtiani, A., Carletta, J., Zhe, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+impedimetric+approach+for+accurate+particle+sizing+using+a+microfluidic+Coulter+counter.">An impedimetric approach for accurate particle sizing using a microfluidic Coulter counter.</a> J Micromech Microeng. 21 (4), 045036 (2011).</li><li>Gold, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optimal+binary+sequences+for+spread+spectrum+multiplexing+(Corresp).">Optimal binary sequences for spread spectrum multiplexing (Corresp).</a> IEEE Trans. Inform. Theory. 13 (4), 619-621 (1967).</li><li>Dinan, E., Jabbari, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Spreading+codes+for+direct+sequence+CDMA+and+wideband+CDMA+cellular+networks.">Spreading codes for direct sequence CDMA and wideband CDMA cellular networks.</a> IEEE Commun Mag. 36 (9), 48-54 (1998).</li></ol>

Çoklu dirençli darbe sensörleri daha önce mikroakışkan cips 28, 29, 30, 31, 32 içine dahil edilmiştir. Bu sistemlerde, dirençli darbe sensörler, 28, 29, 30, 31 çoklanır değildi ya da tek tek sensörler farklı frekansla...

Bu sıvı içinde süspansiyon haline getirilmiş hücreler, bakteri ya da virüsler gibi biyolojik parçacıkların doğru algılama ve analiz uygulamaları, 1, 2, 3, bir dizi büyük ilgi görmektedir. Boyutunda çok iyi bir uyum, mikroakışkan cihazlar, yüksek hassasiyet, yumuşak örnek manipülasyon ve iyi kontrollü mikroçevresinin 4, 5, 6, 7 gibi bu amaç için benzersiz avantajlar sunuyor. Buna ek olarak, mikro-akışkan cihazlar pasif çeşitli özellikleri 8, 9, 10, 11, 12, biyolojik partiküllerin heterojen bir popülasyonu fraksiyonlanması akışkan dinamiği ve kuvvet alanları bir arada kullanılması için tasarlanmış olabilir. Bu cihazdas, sonuçta çıkan partikül dağılımını, okuma çıktısı olarak kullanılabilir, ancak konumsal bilgi bir laboratuar alt için bağlayarak mikroakışkan cihazın pratik yarar sınırlama sadece mikroskobu ile tipik olarak erişilebilir. Bu nedenle, bir mikroakışkan cihaz üzerinde manipüle gibi kolayca, parçacığın zamanmekansal eşleme bildirebilirim entegre sensör, potansiyel olarak düşük maliyetli, mobil numunelerin test edilmesi için özellikle çekici olan entegre lab-on-a-chip cihazlar etkinleştirebilirsiniz , kaynakları sınırlı ayarlar. İnce film elektrotların çeşitli uygulamalar 13, 14 için mikroakışkan cihazlar entegre sensör olarak kullanılmıştır. Dirençli Darbe Algılama (RPS) elektrikli ölçümlerin 15 doğrudan, sağlam, hassas ve yüksek verimli algılama mekanizması sunuyor mikroakışkan kanallarda küçük partiküllerin entegre algılama için caziptir. RPS olarak, bir elektroliti içine daldırılmış bir elektrot çifti arasındaki empedans modülasyonu, bir parçacık tespit etmek için bir araç olarak kullanılır. Parçacık bir delikten geçerken, tanecik mertebesinde boyutlu, sayı ve elektrik akımı, geçici darbelerin genliği, sırasıyla, sayısı ve büyüklüğü parçacıkların için kullanılır. Ayrıca, sensör geometrisi duyarlılığı 16, 17, 18, 19 artırmak için ya mikroakışkan kanal 20 içindeki parçacıkların dikey konumunu tahmin etmek için mukavemet darbesi dalga şekillerini şekil fotolitografik çözünürlükte tasarlanabilir. Biz son zamanlarda Elektrik Algılama (mikroakışkan KODLARI) 21 tarafından mikroakışkan Kodlu Ortogonal Algılama denilen ölçeklenebilir ve basit çoklanmış direnç darbe algılama teknolojisini tanıttı. Mikroakışkan KODLARI bir dayanmaktadırdirenç darbe sensörleri ağından çoklama sağlayacak şekilde her biri bir benzersiz ayırt edici bir şekilde iletimini modüle etmek için mikro boyuttaki elektrotlar bir dizi ihtiva etmektedir. Biz özellikle kod bölmeli çoklu erişim kullanılan dijital kodları benzer dik elektrik sinyalleri üretmek için her sensör tasarladık 22 (CDMA) telekomünikasyon ağları, bireysel rezistif nabız sensörü sinyal benzersiz, tek bir çıkış dalga elde edilebilir ve böylece bile sinyallerinden eğer farklı sensörler müdahale. Bu şekilde, bizim teknolojinin en az aygıta ve sistem düzeyinde daha karmaşık bir tutarken, mikroakışkan çip üzerinde farklı konumlarda parçacıkların izlenmesine izin veren bir 1D elektrik sinyaline parçacıkların 2B uzamsal bilgiler sıkıştırır. Bu yazıda, deneysel ve hesaplamalı mikroakışkan KODLARI teknolojiyi kullanmak için gerekli yöntemler yanı sıra r için ayrıntılı bir protokol mevcutsimüle biyolojik numunelerin analizi kullanımı ile ilgili epresentative oluşur. tekniği açıklamak için bir örnek olarak dört çoklanmış sensörlerle bir prototip cihaz sonuçlarını kullanarak, (2) deney düzeneği açıklama da dahil olmak üzere mikroakışkan KODLARI teknolojisi ile mikroakışkan cihazlar oluşturmak için (1) mikroimalat sürecinde protokolleri sağlamak elektronik, optik, ve akışkan bir donanım, (3) farklı sensörlerden müdahale sinyallerin dekode edilmesi için bir bilgisayar algoritması, ve (4) tespit edilmesi ve mikroakışkan kanal kanser hücrelerinin analiz sonuçları. Biz ayrıntılı bir protokol kullanarak burada anlatılan, diğer araştırmacıların araştırma için bizim teknoloji uygulayabilirsiniz inanıyorum.

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microfluidic platform with multiplexed electronic detection for spatial tracking of particles

Biyolojik numunelerin mikroakışkan işlem tipik olarak, uzaysal ilgi biyolojik bir özelliğine göre örnek damıtmak için çeşitli güç alanı altında asılı parçacıkların ayırıcı işlemlerini içerir. Elde edilen alansal dağılımı deney, okuma çıkışı olarak kullanılabilir için, mikro-akışkan cihazlar genellikle daha yüksek maliyeti ve indirgenmiş taşınabilirlik karmaşık aletleri gerektiren mikroskopik analizine tabi tutulurlar. Bu sınırlama gidermek için, biz bir mikroakışkan çip üzerinde farklı yerlerde parçacıkların çoklanmış tespiti için entegre bir elektronik algılama teknolojisini geliştirdik. Mikroakışkan KODLARI denilen Bizim teknoloji, bir 1D elektrik sinyaline içine 2D uzamsal bilgiyi sıkıştırmak için Kod Bölmeli Çoklu Erişim ile Dirençli Darbe Algılama birleştirir. Bu yazıda, mikroakışkan KODLARI teknolojinin pratik bir gösteri tespit etmek ve boyut kültürlü kanser hücreleri birden mikroakışkan kanallar üzerinden dağıtılan sunuyoruz. Gibiyüksek hızlı mikroskopi tarafından doğrulandığı, teknoloji doğru harici cihaz için gerek kalmadan tüm elektronik yoğun hücre popülasyonlarının analiz edebilirsiniz. Bu nedenle, mikroakışkan KODLARI potansiyel biyolojik örneklerin noktası bakım testleri için uygundur düşük maliyetli entegre lab-on-a-chip cihazlar etkinleştirebilirsiniz.

Dört mikroakışkan kanal üzerinden dağıtılmış dört sensör oluşan mikroakışkan KODU cihazı Şekil 1b &#39;de gösterilmiştir. (1) birden çok hücre paralel ve (2) hücre elektrotları yakın kalır hassasiyetini arttırarak elektrotlar üzerinden geçemez, böylece bu sistemde, her bir mikroakışkan kanal enine kesiti, bir hücre boyutuna yakın olacak şekilde tasarlanmıştır . Her sensör benzersiz 7-bit dijital kodu üretmek için tasarlanmıştır. ...

Watch this Scientific Journal Video about Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles at JoVE.com

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Birden mikroakışkan kanallarda parçacıkların algılama ve boyutlandırma multipleks için, kod bölmeli çoklu erişim (CDMA) ile rezistif darbe algılama (RPS) birleştiren entegre bir yüzey elektrot ağı ile mikroakışkan platformu göstermektedir.

Parçacıkların Mekansal Takip için Çoklanmış Elektronik Algılama ile mikroakışkan Platformu

Microfluidic processing of biological samples typically involves differential manipulations of suspended particles under various force fields in order to ...

The overall goal of this procedure is to demonstrate a microfluidic platform that combines resistive pulse sensing with code division multiple access to multiplex the detection and sizing of particles in multiple microfluidic channels. This technology, named microfluidic CODES can help realize fully integrated and truly portable labware chip devices that are well suited for the point of care testing of biological samples in resources limited settings. The main advantage of this technique is that it can electronically track spatial, temporal manipulation of particles on the microfluidic chip, eliminating the need for an external instrument such as a microscope.Our technology is compatible with soft lithography and can easily be integrated into a microphotic device where particles are fractionated to provide a direct electronic readout similar to Coulter counter. To begin construction of the microfluidic device generate a set of four, seven bit gold codes. Then design four unique electrode layouts based on the gold codes using a computer aided design, or CAD software such as AutoCAD.Finally, have the Photomasked with the designed electrode layout manufactured by a Photomask supplier. Next, soak a four inch borosilicate glass wafer in a five to one piranha solution at 120 degrees Celsius for 20 minutes. After cleaning, heat the wafer on a hot plate at 200 degrees Celsius for 20 minutes to evaporate residual water.Place the clean, dry wafer in a spin coater. Apply 2 milliliters of negative photoresist solution to the wafer and spin coat at 3000 revolutions per minute for 40 seconds. Dry the spin coated wafer on a hot plate at 150 degrees Celsius for one minute.The cover the wafer with a chrome mask in the desired electrode pattern. Expose the masked photoresist surface to 365 nanometer UV light to attain 225 millijoule per centimeter squared. The bake the exposed photoresist on a hot plate at 100 degrees Celsius for one minute.Immerse the wafer in photoresist developer for 15 seconds, then wash the pattern wafer in a gentle spray of deionized water and dry the wafer under a stream of nitrogen gas. Next, place the patterned wafer in an electron beam metal evaporator. Deposit a 20 nanometer thick chrome layer and a 80 nanometer thick gold layer onto the wafer at a rate of one Angstrom per second.Then etch the underlying photoresist by ultrasonic heating the metal coated wafer in acetone for 30 minutes at 40 kilohertz and 100%amplitude. Use a dicing saw to cut the wafer into smaller pieces as needed. To begin fabricating the microfluidic channel mold, clean and dry a four inch silicon wafer in the same way as the borosilicate wafer described previously.Place the silicon wafer in a spin coater and apply four milliliters of negative photoresist solution. Spin coat the wafer at 500 rpm for 15 seconds, then at 1, 000 rpm for 15 seconds, and finally at 3, 000 rpm for 60 seconds. Set the wafer face up on an acetone soaked clean room wipe to remove residual photoresist from the back and the edges of the wafer.Bake the wafer at 65 degrees Celsius for one minute and then at 95 degrees Celsius for two minutes. Place a chrome mask pattern for the microfluidic channels on the dry wafer. Expose the photoresist to 365 nanometer UV light at 180 millijoules per centimeter squared and then bake the wafer again at 65 and 95 degrees Celsius for one and two minutes respectively.Placed the patterned wafer in a container of photoresist developer and gently shake the container for three minutes. Rinse the developed wafer in isopropanol and dry the wafer under a stream of nitrogen gas. Bake the wafer at 200 degrees Celsius for 30 minutes, then use a profilometer to check that the pattern photoresist is uniformly thick across the wafer.Place the wafer in a vacuum desiccator along with 200 microliters of trichlorosilane in an uncovered Petri Dish. Allow the wafer to sit in the desiccator with the trichlorosilane for eight hours to silanize the wafer surface. To begin assembling the device, use general purpose clean room tape to affix the silicon wafer mold in a 150 millimeter diameter Petri Dish.Add 50 grams of a 10 to one mixture of polydimethylsiloxane prepolymer to the Petri Dish and degas the mixture in a vacuum desiccator for one hour. Cure the degassed mixture at 65 degrees Celsius for at least four hours. Use a scalpel to cut out the cured PDMS layer and then peel the cured layer off the mold with tweezers.Cut the PDMS into small pieces. Punch the inlet and outlet microfluidic channel holes with a biopsy puncher. Place the PDMS layer pattern face down on clear room tape to clean the micromachined surface.Rinse the previously prepared electrode bearing glass substrate with acetone, isopropanol, and deionized water. Dry the substrate under a stream of nitrogen gas. Place the PDMS layer and the substrate in an RF plasma generator set to 100 milliwatts with the micromachine sides facing up.Activate the micromachine surfaces in oxygen plasma for 30 seconds. Next use an optical microscope to align the pattern PDMS layer with the surface electrodes. Once aligned, allow the surfaces to come into physical contact to seal the PDMS layer onto the glass substrate.It is crucial that the coating electrode pattern on glass substrate is properly aligned with the PDMS microfluidic channels. Once aligned properly, the particle interaction with the surface electrode will generate a desired code waveform for multiplexing. Bake the assembled device at 70 degrees Celsius for five minutes, glass side down.Finally solder wires to the electrode contact pads to finish device assembly. To begin the experiment, place the microfluidic device on an optical microscope stage. Connect the device reference electrode to the signal output port of a lock-in amplifier and apply a 400 kilohertz sine wave.Connect the positive and negative sensor electrodes to two independent transimpedance amplifiers. Connect both transimpedance amplifiers to the differential voltage inputs of the lock-in amplifier with the positive sensor signal to be subtracted from the negative sensor signal. Connect the demodulator output of the lock-in amplifier to a data acquisition unit.In the data acquisition software, set a sampling rate for the lock-in amplifier output of 1 Megahertz. Set up a high speed camera to optically record the operation of the device as seen under the microscope. Draw a prepared cell suspension into a syringe.Secure the sample syringe in a syringe pump and connect the syringe to the inlet channel. Direct the outlet channel to a waste container. Use the syringe pump to drive the cell suspension through the device at a constant flow rate while recording the impedance modulation signal.After experiment's completion, process the electrical data with analysis software. Compare the processed electrical signal with images from the high speed camera to create a calibration curve for cell size. A cell suspension was flowed through a microfluidic sensor device with four unique electrode patterns derived from orthogonal sensor codes.All four sensor signals were recorded from a single electrical output. The individual sensor associated with each recorded signal was identified by correlation of the recorded sensor signals with all possible codes, which produced clearly distinguishable, autocorrelation peaks. Waveforms produced by the interfering signals from simultaneous detection of cells in all four channels were resolved with an iterative algorithm.A recorded waveform was correlated with all possible codes and the largest autocorrelation peak was identified. The corresponding individual sensor signal was reconstructed and subtracted from the input waveform. The residual signal was passed to the next iteration as the input and the process continued until the residual signal produced no autocorrelation peaks.Estimated signals were refined based on an optimization algorithm seeking the best fit between the reconstructed and the original recorded waveforms using the least squares approximation. Cell location, size, and time to cross the sensor were then determined from the channel number, amplitude, duration, and relative timing of the estimated sensor signals. The procedure was validated by comparison of the electrical signals with optical measurements from the high speed camera.Once mastered, this technique is very easy to implement, because it is very simple from a hardware perspective. It has no active on-chip component. It is directly compatible with soft lithography and the signal processing relies on a simple computational algorithm.Following this protocol you can fabricate microfluidic chips with code based multiplex electrical sensors and decode electrical signals for bioanalytical measurement. This versatile, scalable, electronic sensing technology, can be readily integrated into various microfluidic devices to realize quantitative assays by spatial temporally tracking particles as they processed on the chip. After watching this vide, you should have a good understanding of how to design, fabricate, and implement a microfluidic CODES technology.

Research

JoVE Journal

Bioengineering

A Microfluidic-based Hydrodynamic Trap for Single Particles

Tek Parçacıklar için mikroakışkan tabanlı Hidrodinamik Tuzak

The ability to confine and manipulate single particles in free solution is a key enabling technology for fundamental and applied science.  Methods for ...

Biyomühendislik

Fluorescence detection methods for microfluidic droplet platforms

Mikroakışkan damlacık platformları için Floresan algılama yöntemleri

The development of microfluidic platforms for performing chemistry and biology has in large part been driven by a range of potential benefits that ...

Bridging the Bio-Electronic Interface with Biofabrication

Biofabrication ile Bio-Elektronik Arayüz Bridging

Advancements in lab-on-a-chip technology promise to revolutionize both research and medicine through lower costs, better sensitivity, portability, and ...

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Sağlam, mikroakışkan Hücre içi Delivery Platform olarak hücre Sıkma

Rapid mechanical deformation of cells has emerged as a promising, vector-free method for intracellular delivery of macromolecules and nanomaterials. This ...

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood

İşlenmemiş kandan Nötrofil Kemotaksis Ölçüm mikroakışkan Platformu

Neutrophils play an essential role in protection against infections and their numbers in the blood are frequently measured in the clinic. Higher ...

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Bir mikroakışkan Hassas Küçük hacimli Numune İşleme Platformu ve Akustik mikrocihazda Ayrı Biyolojik Parçacıklar Boyutu Kullanımı

A major advantage of microfluidic devices is the ability to manipulate small sample volumes, thus reducing reagent waste and preserving precious sample. ...

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Yüksek verim Tek hücreli İzolasyonu ve Kültür mikroakışkan Platformu

Studying the heterogeneity of single cells is crucial for many biological questions, but is technically difficult. Thus, there is a need for a simple, yet ...

Assembly and Tracking of Microbial Community Development within a Microwell Array Platform

Bir Mikro Kuyruklu Dizi Platformunda Mikrobik Topluluk Geliştirmenin Montajı ve Takibi

The development of microbial communities depends on a combination of complex deterministic and stochastic factors that can dramatically alter the spatial ...

Imaging the Root Hair Morphology of Arabidopsis Seedlings in a Two-layer Microfluidic Platform

Imaging the Root Hair Morphology of Arabidopsis Seedlings in a Two-layer Microfluidic Platform

Kök saç Morfoloji Arabidopsis fidan bir iki katlı mikrosıvısal platformda görüntüleme

Root hairs increase root surface area for better water uptake and nutrient absorption by the plant. Because they are small in size and often obscured by ...

A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans

A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans

Boyuna Imaging'de Caenorhabditis elegans için bir mikrosıvısal Platform

In the last decade, microfluidic techniques have been applied to study small animals, including the nematode Caenorhabditis elegans, and have proved ...