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DOI :
10.3791/55315-v
April 14th, 2017
Chapters
0:05
Title
1:13
Isolation of Neurospheres from the Adult Mouse Brain
2:43
Embedding and Sectioning of Neurospheres for immunostaining
4:10
Starvation and Analysis of Cilia
6:01
Results: Starvation Induces Ciliogenesis in Dissociated, Adherent Neutral Stem/Progenitor Cells
7:19
Conclusion
初级纤毛是在神经祖细胞增殖,神经细胞的分化,和成人神经元功能至关重要的。这里,我们描述研究ciliogenesis和信号蛋白使用初级神经球培养物在神经干/祖细胞和分化的神经元纤毛的贩卖的方法。
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