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DOI :
10.3791/55333-v
March 4th, 2017
Chapters
0:05
Title
1:20
Generation of Fluorescent Protein Cassettes by PCR
3:26
Transform Candida Cells with Fluorescent Protein DNA Cassette
7:31
Results: Generation of GFP and mCherry Fusions to Eno1
8:46
Conclusion
PCR 매개 유전자 변형은 시각화 및 효모 세포 및 단백질의 정량을 용이 칸디다 종의 형광 단백질 융합체를 생성하기 위해 사용될 수있다. 여기서, 우리는 칸디다에서 형광 단백질 융합 (Eno1-FP)을 구축하기위한 전략을 제시한다.
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