Name: measuring and modeling contractile drying in human stratum corneum
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En fritaget godkendelse (3002-13) at udføre forskning ved hjælp vævsprøver de-identificeret i henhold til Department of Health and Human Services regulativer, 45 CFR 46,101 (b) (4) blev ydet. Fuld tykkelse hud er modtaget fra elektiv kirurgi. I denne artikel, vævet kilden er 66 år gammel kaukasisk kvindelige bryst. 1. Udarbejdelse af Elastomer Coated Dækglas <ol><li> I en 20 ml glasbeholder, blandes 0,107 g Sylgard 184 hærder med 5,893 g base. Den totale blanding masse er 6 g med e...

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I denne artikel beskriver vi en teknik, der kan anvendes til at måle den dynamiske tørring adfærd og mekaniske egenskaber af humant SC. Tidligere undersøgelser har vist, at denne teknik kan bruges til at kvantificere effekten af miljøforhold og kemiske produkter, der almindeligvis anvendes i kosmetiske rensemidler og fugtighedscreme på den dynamiske tørring adfærd SC 7, 8. Der er en række centrale trin i protokollen. For det første, SC svulmer navnlig ...

Det yderste lag af epidermis, eller stratum corneum (SC) består af kohæsive corneocyt celler omgivet af et lipid rig matrix 1, 2. Sammensætningen og strukturelle integritet af SC er afgørende for at opretholde korrekt barriere funktionalitet 3, som forhindrer invasion fra mikroorganismer og modstår både mekaniske kræfter og stort væsketab fire. Kapaciteten af produkter til personlig pleje for at opretholde eller nedbryde hudens barrierefunktion er af stor interesse for huden sundhedspleje og den kosmetiske industri fem. Den daglige anvendelse af personlig pleje-produkter vides at ændre de mekaniske egenskaber af SC 6, 7, 8. For eksempel kan Tensiderne i kosmetiske rensemidler forårsage betydelige stigninger i elasticitetsmodul og en ophobning aftørring spændinger i SC, stigende vævet tilbøjelighed til at knække 7, 9. Glycerol indeholdt i næsten alle kosmetiske moisturizers kan blødgøre SC og mindske opbygningen af tørrebelastninger 8, 10, 11, hvilket reducerer sandsynligheden for væv brud. Metoden beskrevet i denne artikel er i stand til at kvantificere den dynamiske tørring adfærd og mekaniske egenskaber af SC tørring i kontrollerede miljøer 7, 8. Tidligere har denne teknik vist sig at være i stand til at belyse effekten af ​​forskellige kosmetiske produkter på ændringer i den dynamiske tørring adfærd og mekaniske egenskaber af SC væv. Dette opnås ved at kvantificere tørring-induceret krympning af humant SC væv klæber til en blød elastomer substrat, montering tørring forskydninger med en simpelkontraktilitet model, og derefter ekstrahere elasticitetsmodulet og tørring stress fra den monterede profil. Når test af flere SC prøver er påkrævet, denne metode giver en hurtigere alternativ til enaksede tensometry, udnytter betydeligt mindre væv og giver mere fysiologisk relevant tørring ved at forhindre fordampning fra prøven underside.

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	<tbody>
		<tr>
			<td>Silicone elastomer base</td>
			<td>Dow-Corning</td>
			<td>1064291</td>
		</tr>
		<tr>
			<td>Silicone elastomer Curing Agent</td>
			<td>Dow-Corning</td>
			<td>1015311</td>
		</tr>
		<tr>
			<td>FluoSpheres Carboxylate 0.1 &#181;m yellow green fluorescent 505/515&#160;</td>
			<td>Thermo Fisher</td>
			<td>F8803</td>
		</tr>
		<tr>
			<td>FluoSpheres Carboxylate 1 &#181;m yellow green fluorescent 505/515&#160;</td>
			<td>Thermo Fisher</td>
			<td>F8823</td>
		</tr>
		<tr>
			<td>FluoSpheres Carboxylate 1 &#181;m nile red fluorescent 535/575&#160;</td>
			<td>Thermo Fisher</td>
			<td>F8819</td>
		</tr>
		<tr>
			<td>Trypsin from porcine pancreas</td>
			<td>Sigma-Aldrich</td>
			<td>T6567</td>
		</tr>
		<tr>
			<td>Trypsin inhibitor type II-s</td>
			<td>Sigma-Aldrich</td>
			<td>T9128</td>
		</tr>
		<tr>
			<td>(3-aminopropyl)triethoxysilane</td>
			<td>Sigma-Aldrich</td>
			<td>440140</td>
		</tr>
		<tr>
			<td>Sodium tetraborate</td>
			<td>Sigma-Aldrich</td>
			<td>221732</td>
		</tr>
		<tr>
			<td>Boric acid</td>
			<td>Sigma-Aldrich</td>
			<td>B0294</td>
		</tr>
		<tr>
			<td>Phosphate buffered saline</td>
			<td>Sigma-Aldrich</td>
			<td>P7059</td>
		</tr>
		<tr>
			<td>N-(3-Dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride&#160;</td>
			<td>Sigma-Aldrich</td>
			<td>E7750</td>
		</tr>
		<tr>
			<td>Vortexer mixer</td>
			<td>VWR</td>
			<td>58816-123</td>
		</tr>
		<tr>
			<td>6mm diameter hole punch</td>
			<td>Sigma-Aldrich</td>
			<td>Z708860</td>
		</tr>
		<tr>
			<td>SOLA 6-LCR-SB&#160;</td>
			<td>Lummencor light engine</td>
			<td>No.3526</td>
		</tr>
		<tr>
			<td>Cfi Plan Achro Uw 1x Objective</td>
			<td>Nikon Plan UW</td>
			<td>MRL00012</td>
		</tr>
		<tr>
			<td>CFI Plan Fluor 40x Oil Objective 1.3 na - 0.20mm wd</td>
			<td>Nikon Plan Fluor</td>
			<td>MRH01401</td>
		</tr>
		<tr>
			<td>Nikon Eclipse Ti-U inverted microscope&#160;</td>
			<td>Nikon</td>
			<td>MEA53200</td>
		</tr>
		<tr>
			<td>Clara-E Camera</td>
			<td>Andor</td>
			<td>DR-328G-C02-SIL</td>
		</tr>
		<tr>
			<td>Remote Focus Attachment E-RFA Ergo Design</td>
			<td>Nikon</td>
			<td>99888</td>
		</tr>
		<tr>
			<td>Ti-S-E Motorized Stage</td>
			<td>Nikon</td>
			<td>MEC56110</td>
		</tr>
	</tbody>
</table>

measuring and modeling contractile drying in human stratum corneum

Stratum corneum (SC) er den mest overfladiske hudlag. Dens kontakt med det eksterne miljø betyder, at dette væv lag underkastes både rengøringsmidler og daglige variationer i omgivende fugtighed; begge kan ændre indholdet af vævet vand. Reduktioner i vandindholdet af svær barriere dysfunktion eller lav fugtighed miljøer kan ændre SC stivhed og forårsage en ophobning af tørring spændinger. Under ekstreme forhold kan disse faktorer forårsage mekanisk brud på vævet. Vi har etableret et high throughput metode til kvantificering dynamiske ændringer i de mekaniske egenskaber af SC efter tørring. Denne teknik kan anvendes til at kvantificere ændringer i tørring adfærd og mekaniske egenskaber af SC med kosmetiske cleanser og fugtighedscreme behandlinger. Dette opnås ved at måle dynamiske variationer i rumligt opløst i planet tørring forskydninger af cirkulære vævsprøver klæbet til en elastomer substrat. In-plane radiale forskydninger ACQgendannes for under tørring er azimutalt udlignes og forsynet med en profil baseret på en lineær elastisk kontraktilitet model. Dynamiske ændringer i tørring stress og SC elasticitetsmodul kan derefter udvindes fra den model profiler.

Figur 1 (a) viser et repræsentativt fluorescens billede af en SC prøve overtrukket med fluorescerende perler (afsnit 3). Den tilsvarende transmitterede lys billede af prøven er vist i figur 1 (b) overlejret med et pilekogger plot af rumligt opløst tørring forskydninger, der danner efter 16 h tørring ved 25% RH grund af den cirkulære symmetri af prøverne, kan disse forskydninger være azimutalt gennemsnit. Figur 1 (c) viser radial...

Watch this Scientific Journal Video about Measuring and Modeling Contractile Drying in Human Stratum Corneum at JoVE.com

Measuring and Modeling Contractile Drying in Human Stratum Corneum

Denne artikel beskriver en fremgangsmåde til kvantificering af dynamiske tørring adfærd og mekaniske egenskaber af stratum corneum ved måling rumligt opløst i planet tørring forskydninger af cirkulære vævsprøver klæbet til en elastomer substrat. Denne teknik kan anvendes til at måle, hvordan forskellige kemiske behandlinger ændrer tørring og vævs mekaniske egenskaber.

Måling og modellering kontraktile Tørring i Human stratum corneum

Stratum corneum (SC) is the most superficial skin layer. Its contact with the external environment means that this tissue layer is subjected to both ...

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