January 28th, 2017
•Here, we describe a method for the isolation of cells in the pancreas microenvironment from embryonic, neonatal and adult mouse tissue, focusing on the isolation of mesenchymal cells. This method allows profiling of cell gene expression and protein secretion in order to elucidate the extrinsic signals that regulate pancreas development, function, and tumorigenesis.
Tags
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved