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DOI :
10.3791/55349-v
March 26th, 2017
Chapters
0:05
Title
1:03
Reconstitution of a Histone Octamer with Differentially Isotope-labeled and Asymmetrically-modified Histone H3
3:20
Tandem Affinity Purification of Reconstituted Octamer
5:28
Nucleosome Reconstitution
7:07
Results: Generation of Differentially Isotope-labeled Nucleosomes for Investigating Cross-talk Mechanisms
8:58
Conclusion
这个协议描述了含有差异同位素标记的妹妹组蛋白核小体重构。同时,可使用premodified组蛋白拷贝之后产生不对称翻译后修饰的核小体。这些制剂可以很容易地用于研究修改串扰机制,同时在两个姐姐组蛋白,通过使用高分辨率核磁共振光谱。
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