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DOI :
10.3791/55360-v
•
8:46 min
March 7th, 2017
Chapters
0:05
Title
1:12
Mechanical Dissociation
3:25
Enzymatic Dissociation
4:50
Sucrose Gradient Centrifugation
5:49
ACK Red Blood Cell Lysis and Initial Culture Maintenance
6:50
Results: Images of Primary Cultures and Early-passage Cells
7:41
Conclusion
本协议描述为事后漫内在脑桥脑胶质瘤样品进行建立以病人来源的细胞培养模型或肿瘤微环境和细胞的直接表征的快速处理的方法。
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