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DOI :
10.3791/55360-v
•
8:46 min
March 7th, 2017
Chapters
0:05
Title
1:12
Mechanical Dissociation
3:25
Enzymatic Dissociation
4:50
Sucrose Gradient Centrifugation
5:49
ACK Red Blood Cell Lysis and Initial Culture Maintenance
6:50
Results: Images of Primary Cultures and Early-passage Cells
7:41
Conclusion
このプロトコルは、患者由来の細胞培養モデルまたは腫瘍および微小環境細胞の直接的な特徴付けの確立のための死後拡散固有橋グリオーマサンプルを迅速に処理するための方法を記載します。
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