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DOI :
10.3791/55360-v
•
8:46 min
March 7th, 2017
Chapters
0:05
Title
1:12
Mechanical Dissociation
3:25
Enzymatic Dissociation
4:50
Sucrose Gradient Centrifugation
5:49
ACK Red Blood Cell Lysis and Initial Culture Maintenance
6:50
Results: Images of Primary Cultures and Early-passage Cells
7:41
Conclusion
이 프로토콜은 환자 유래의 세포 배양 모델 또는 종양 미세 환경과 세포의 직접적인 특성화 확립 사후 확산 극한 뇌교 교종 샘플들의 빠른 처리를위한 방법을 설명한다.
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