Name: simultaneous evaluation of cerebral hemodynamics and light scattering properties of the in vivo rat brain using multispectral diffuse reflectance imaging
Uploaded: 2017-05-07T15:00:00
Description: Watch this Scientific Journal Video about Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging at J

Part of this work was supported by a Grant-in-Aid for Scientific Research (C) from the Japanese Society for the Promotion of Science (25350520, 22500401, 15K06105) and the US-ARMY ITC-PAC Research and Development Project (FA5209-15-P-0175).

Dierverzorging, voorbereiding, en experimentele protocollen werden goedgekeurd door het Comité van Tokyo University of Agriculture and Technology Animal Research. Voor deze methode wordt de rat gehuisvest in een gecontroleerd milieu (24 ° C, 12 uur licht / donkercyclus) met voedsel en water beschikbaar ad libitum. 1. Bouw van een conventionele Multispectral Diffuse reflectie Imaging System <ol><li> Mount negen smalbandige interferentiefilters met centrale golflengten van 500, 52...

<ol>
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De meest kritische stap in dit protocol is de verwijdering van de verdunde schedel regio craniale venster maken; Dit moet zorgvuldig worden uitgevoerd om onverwachte bloeden te voorkomen. Deze stap is belangrijk voor het verkrijgen van hoge kwaliteit multispectrale diffuse reflectie beelden met een hoge nauwkeurigheid. Het gebruik van een stereomicroscoop wordt aanbevolen voor de chirurgische procedure, indien mogelijk. Kleine stukjes van gelatine spons zijn nuttig voor hemostase. Het optisc...

Multispectrale diffuse reflectie beeldvorming is de meest gebruikelijke techniek voor het verkrijgen van een ruimtelijke kaart van intrinsieke optische signalen (IOSs) in corticale weefsel. IOSs waargenomen in de in vivo hersenen worden vooral toegeschreven aan drie fenomenen: variaties in lichtabsorptie en verstrooiing eigenschappen als gevolg corticale hemodynamica, variatie van de absorptie afhankelijk van de reductie of oxidatie van cytochromen in mitochondria en variaties in lichtverspreidingeigenschappen geïnduceerd door morfologische veranderingen 1. Licht in het zichtbare (VIS) tot nabij-infrarood (NIR) spectrum effectief geabsorbeerd en verstrooid door biologisch weefsel. Het diffuse reflectiespectrum van de in vivo hersenen wordt gekenmerkt door absorptie en verstrooiing spectra. De gereduceerde verstrooiing coëfficiënten ps &#39;hersenweefsel in het golflengtegebied resultaat VIS-NIR naar een monotone verstrooiing spectrum exhibiting lagere magnitude bij langere golflengten. De gereduceerde verstrooiingscoëfficiënt spectrum μ s &#39;(λ) kan worden gebracht met de vorm van de machtswet functie 2, 3 en μ s&#39; (λ) = a x λ -b. De verstrooiing b is gerelateerd aan de omvang van biologische verspreiders in levend weefsel 2, 3. Morfologische veranderingen in het weefsel en afname van de levensvatbaarheid van levend corticale weefsel kan de omvang van de biologische verstrooiers 4, 5, 6, 7, 8, 9 beïnvloeden. Een optisch systeem voor multispectrale diffuse reflectie beeldvorming kan eenvoudig worden opgebouwd uit een gloeilamp livechten bron, eenvoudige optische componenten en een monochromatische geladen gekoppelde inrichting (CCD). Daarom zijn diverse algoritmen en optische systemen multispectrale diffuse reflectie beeldvorming gebruikt voor corticale hemodynamica en / of weefselmorfologie 10, 11, 12, 13, 14, 15, 16, 17, 18 te evalueren. De werkwijze beschreven in dit artikel wordt gebruikt om zowel de hemodynamica en lichtverstrooiende eigenschappen van rat cerebrale weefsel in vivo toepassing van een gebruikelijke multispectrale diffuse reflectie-afbeeldingssysteem visualiseren. De voordelen van deze werkwijze boven alternatieve technieken de mogelijkheid tijdruimtelijke veranderingen in zowel cerebrale hemodynamiek en corticaal weefsel evaluerenmorfologie, evenals de toepasselijkheid ervan op verschillende hersendisfunctie diermodellen. Daarom zal de methode die geschikt is voor het onderzoek van traumatisch hersenletsel, epilepsie, beroerte en ischemie.

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		<col />
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	<tbody>
		<tr height="36">
			<td height="36" style="height:36px;width:289px;">150-W halogen-lamp light source</td>
			<td style="width:281px;">Hayashi Watch Works Co., Ltd, Tokyo, Japan</td>
			<td style="width:119px;">LA-150SAE</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="36">
			<td height="36" style="height:36px;">Light guide</td>
			<td style="width:281px;">Hayashi Watch Works Co., Ltd, Tokyo, Japan</td>
			<td>LGC1-5L1000</td>
			<td></td>
		</tr>
		<tr height="36">
			<td height="36" style="height:36px;">Collecting lens</td>
			<td style="width:281px;">Hayashi Watch Works Co., Ltd, Tokyo, Japan</td>
			<td>SH-F16</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@500nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65088</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@520nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65093</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@540nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65096</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@560nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#67766</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@570nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#67767</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@580nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65646</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@600nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65102</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@730nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65115</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@760nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#67777</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Motorized filter wheel&#160;</td>
			<td>Andover Corporation, NH, USA</td>
			<td>FW-MOT-12.5</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">16-bit cooled CCD camera</td>
			<td>Bitran, Japan</td>
			<td>BS-40</td>
			<td></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;">Video zoom lens</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>VZMTM300i</td>
			<td></td>
		</tr>
		<tr height="36">
			<td height="36" style="height:36px;width:289px;">Spectralon white standard with 99% diffuse reflectance</td>
			<td>Labsphere Incorporated, North Sutton, NH, USA</td>
			<td>SRS-99-020</td>
			<td></td>
		</tr>
	</tbody>
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simultaneous evaluation of cerebral hemodynamics and light scattering properties of the in vivo rat brain using multispectral diffuse reflectance imaging

The simultaneous evaluation of cerebral hemodynamics and the light scattering properties of in vivo rat brain tissue is demonstrated using a conventional multispectral diffuse reflectance imaging system. This system is constructed from a broadband white light source, a motorized filter wheel with a set of narrowband interference filters, a light guide, a collecting lens, a video zoom lens, and a monochromatic charged-coupled device (CCD) camera. An ellipsoidal cranial window is made in the skull bone of a rat under isoflurane anesthesia to capture in vivo multispectral diffuse reflectance images of the cortical surface. Regulation of the fraction of inspired oxygen using a gas mixture device enables the induction of different respiratory states such as normoxia, hyperoxia, and anoxia. A Monte Carlo simulation-based multiple regression analysis for the measured multispectral diffuse reflectance images at nine wavelengths (500, 520, 540, 560, 570, 580, 600, 730, and 760 nm) is then performed to visualize the two-dimensional maps of hemodynamics and the light scattering properties of the in vivo rat brain.

Representatieve spectrale beelden van diffuse reflectie verkregen uit in vivo rattenhersenen zijn weergegeven in figuur 3. De beelden 500, 520, 540, 560, 570 en 580 nm duidelijk zichtbaar een dicht netwerk van bloedvaten in de cerebrale cortex. De verslechtering van het contrast tussen de bloedvaten en het omliggende weefsel waargenomen in de beelden 600, 730 en 760 nm weerspiegelt de lagere lichtabsorptie door hemoglobine bij langere en NIR golflengten. <p ...

Watch this Scientific Journal Video about Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging at J

Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging

De gelijktijdige evaluatie van cerebrale hemodynamica en de lichtverstrooiende eigenschappen van in vivo rattenhersenweefsel wordt aangetoond met conventionele multispectrale diffuse reflectie-afbeeldingssysteem.

Gelijktijdige Evaluatie van Cerebrale hemodynamiek en Light Scattering Eigenschappen van de<em&gt; In Vivo</em&gt; Rat Brain behulp Multispectral Diffuse reflectie Imaging

The simultaneous evaluation of cerebral hemodynamics and the light scattering properties of in vivo rat brain tissue is demonstrated using a conventional ...

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Neuroscience

Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging

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