Name: simultaneous evaluation of cerebral hemodynamics and light scattering properties of the in vivo rat brain using multispectral diffuse reflectance imaging
Uploaded: 2017-05-07T15:00:00
Description: Watch this Scientific Journal Video about Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging at J

Part of this work was supported by a Grant-in-Aid for Scientific Research (C) from the Japanese Society for the Promotion of Science (25350520, 22500401, 15K06105) and the US-ARMY ITC-PAC Research and Development Project (FA5209-15-P-0175).

Tierpflege, Vorbereitung und experimentelle Protokolle wurden von der Animal Research Committee der Tokyo University of Agriculture and Technology genehmigt. Für diese Methode wird die Ratte in einer kontrollierten Umgebung (24 ° C, 12 h Licht / Dunkel - Zyklus), mit Futter und Wasser ad libitum untergebracht. 1. Aufbau eines herkömmlichen Multispektrale Diffuse Reflectance Imaging System <ol><li> Halterung neun schmalbandigen optische Interferenzfilter mit Mittenwellenlängen ...

<ol>
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Der wichtigste Schritt in diesem Protokoll ist die Entfernung der ausgedünnten Schädel Region, um den Schädel Fenster zu machen; dies sollte sorgfältig durchgeführt werden, um unerwartete Blutungen zu vermeiden. Dieser Schritt ist wichtig für den Erhalt hochwertige multispektraler Reflexionsbilder mit hohen Genauigkeit diffundieren. Die Verwendung eines Stereomikroskops ist für den chirurgischen Eingriff, wenn möglich empfohlen. Kleine Stücke von Gelatineschwamm sind nützlich für die Hämostase. <p class=...

Multispektraler diffuse Reflektanz-Bildgebung ist die am weitesten verbreitete Technik für eine räumliche Karte der intrinsischen optischen Signale (ioss) in kortikale Gewebe zu erhalten. Ioss beobachtete in dem in - vivo - Gehirn wird hauptsächlich in drei Phänomene zurückzuführen: Variationen in der Lichtabsorption und Streueigenschaften aufgrund kortikaler Hämodynamik, Variation in der Absorption auf der Reduktion oder Oxidation von Cytochromen in Mitochondrien abhängig, und Änderungen in den Lichtstreuungseigenschaften durch morphologische Veränderungen induzierten 1. Licht im sichtbaren (VIS) bis zum nahen Infrarot (NIR) Spektralbereich absorbiert und wird wirksam durch biologisches Gewebe verstreut. Das diffuse Reflexionsspektrum des in - vivo - Gehirns wird durch Absorption und Streuung Spektren charakterisiert. Die reduzierten Streukoeffizienten us &#39;von Hirngewebe im VIS-to-NIR - Wellenlängenbereich in Folge einer monotonen Streuspektrum Exhibiting kleinere Größen bei längeren Wellenlängen. Der reduzierte Streukoeffizient μ Spektrum S &#39;(λ) angenähert werden kann in der Form der Potenzfunktion 2, 3 zu sein , wie μ s&#39; (λ) = a × λ -B. Die Streukraft b wird in lebendem Gewebe 2, 3 auf die Größe des biologischen Streuers verwendet. Morphologische Veränderungen des Gewebes und die Verringerung der Lebensfähigkeit von lebendem Gewebe kortikalen können die Größe der biologischen Streuer beeinflussen 4, 5, 6, 7, 8, 9. Ein optisches System zur multispektralen Abbildungs ​​diffusen Reflektanz kann leicht von einer Glühlampe Li konstruiert werdenght Quelle, einfache optische Komponenten und eine monochromatische ladungsgekoppelten Vorrichtung (CCD). Daher wurden verschiedene Algorithmen und optische Systeme für multispektraler diffuse Reflektanz Bildgebung verwendet , um kortikalen Hämodynamik und / oder Gewebemorphologie 10, 11, 12, 13, 14, 15, 16, 17, 18 auszuwerten. Das Verfahren in diesem Artikel beschrieben wird , verwendet , um sowohl die Hämodynamik und Lichtstreuungseigenschaften von Rattenhirngeweben in vivo sichtbar zu machen ein herkömmliches multispektraler diffusen Reflexions - Bildgebungssystem verwendet wird . Die Vorteile dieses Verfahrens gegenüber alternativen Techniken sind die Fähigkeit, Raum-Zeit-Änderungen sowohl zerebrale Hämodynamik und Rindengewebe zu bewertenMorphologie sowie ihre Anwendbarkeit auf verschiedene Funktionsstörungen des Gehirns Tiermodellen. Daher wird das Verfahren zur Untersuchung von traumatischen Hirnverletzungen, epileptischen Anfall, Schlaganfall und Ischämie geeignet sein.

<table border="0" cellpadding="0" cellspacing="0" width="856">
	<colgroup>
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		<col />
		<col />
		<col />
	</colgroup>
	<tbody>
		<tr height="36">
			<td height="36" style="height:36px;width:289px;">150-W halogen-lamp light source</td>
			<td style="width:281px;">Hayashi Watch Works Co., Ltd, Tokyo, Japan</td>
			<td style="width:119px;">LA-150SAE</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="36">
			<td height="36" style="height:36px;">Light guide</td>
			<td style="width:281px;">Hayashi Watch Works Co., Ltd, Tokyo, Japan</td>
			<td>LGC1-5L1000</td>
			<td></td>
		</tr>
		<tr height="36">
			<td height="36" style="height:36px;">Collecting lens</td>
			<td style="width:281px;">Hayashi Watch Works Co., Ltd, Tokyo, Japan</td>
			<td>SH-F16</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@500nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65088</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@520nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65093</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@540nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65096</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@560nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#67766</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@570nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#67767</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@580nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65646</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@600nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65102</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@730nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65115</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@760nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#67777</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Motorized filter wheel&#160;</td>
			<td>Andover Corporation, NH, USA</td>
			<td>FW-MOT-12.5</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">16-bit cooled CCD camera</td>
			<td>Bitran, Japan</td>
			<td>BS-40</td>
			<td></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;">Video zoom lens</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>VZMTM300i</td>
			<td></td>
		</tr>
		<tr height="36">
			<td height="36" style="height:36px;width:289px;">Spectralon white standard with 99% diffuse reflectance</td>
			<td>Labsphere Incorporated, North Sutton, NH, USA</td>
			<td>SRS-99-020</td>
			<td></td>
		</tr>
	</tbody>
</table>

simultaneous evaluation of cerebral hemodynamics and light scattering properties of the in vivo rat brain using multispectral diffuse reflectance imaging

The simultaneous evaluation of cerebral hemodynamics and the light scattering properties of in vivo rat brain tissue is demonstrated using a conventional multispectral diffuse reflectance imaging system. This system is constructed from a broadband white light source, a motorized filter wheel with a set of narrowband interference filters, a light guide, a collecting lens, a video zoom lens, and a monochromatic charged-coupled device (CCD) camera. An ellipsoidal cranial window is made in the skull bone of a rat under isoflurane anesthesia to capture in vivo multispectral diffuse reflectance images of the cortical surface. Regulation of the fraction of inspired oxygen using a gas mixture device enables the induction of different respiratory states such as normoxia, hyperoxia, and anoxia. A Monte Carlo simulation-based multiple regression analysis for the measured multispectral diffuse reflectance images at nine wavelengths (500, 520, 540, 560, 570, 580, 600, 730, and 760 nm) is then performed to visualize the two-dimensional maps of hemodynamics and the light scattering properties of the in vivo rat brain.

Repräsentative Spektralbilder von diffusen Reflexionsvermögen von in - vivo - Rattenhirnen gewonnen sind in Abbildung 3 Die Abbildungen gezeigt bei 500, 520, 540, 560, 570 und 580 nm deutlich , ein dichtes Netz von Blutgefäßen in der Hirnrinde visualisieren. Die Verschlechterung des Kontrastes zwischen Blutgefßen und den umgebenden in den Bildern beobachteten Gewebe bei 600, 730 und 760 nm reflektiert die geringere Absorption von Licht durch Hämog...

Watch this Scientific Journal Video about Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging at J

Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging

Die gleichzeitige Auswertung von zerebralen Hämodynamik und den Lichtstreuungseigenschaften der in vivo Rattenhirngeweben wird ein herkömmliches multispektraler diffuses Reflexions - Bildgebungssystem unter Verwendung demonstriert.

Die gleichzeitige Auswertung der zerebralen Hämodynamik und Lichtstreuungseigenschaften der<em&gt; In Vivo</em&gt; Rattenhirn Mit Multispektral Diffuse Reflectance Imaging

The simultaneous evaluation of cerebral hemodynamics and the light scattering properties of in vivo rat brain tissue is demonstrated using a conventional ...

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Neuroscience

Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging

Focal Cerebral Ischemia Model by Endovascular Suture Occlusion of the Middle Cerebral Artery in the Rat

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