Name: simultaneous evaluation of cerebral hemodynamics and light scattering properties of the in vivo rat brain using multispectral diffuse reflectance imaging
Uploaded: 2017-05-07T15:00:00
Description: Watch this Scientific Journal Video about Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging at J

Part of this work was supported by a Grant-in-Aid for Scientific Research (C) from the Japanese Society for the Promotion of Science (25350520, 22500401, 15K06105) and the US-ARMY ITC-PAC Research and Development Project (FA5209-15-P-0175).

पशु की देखभाल, तैयारी, और प्रयोगात्मक प्रोटोकॉल एग्रीकल्चर एंड टेक्नोलॉजी के टोक्यो विश्वविद्यालय के पशु अनुसंधान समिति द्वारा अनुमोदित किया गया। इस पद्धति के लिए, चूहा भोजन और पानी उपलब्ध यथेच्छ</...

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इस प्रोटोकॉल में सबसे महत्वपूर्ण कदम कपाल खिड़की बनाने के लिए thinned खोपड़ी क्षेत्र को हटाने है; यह अनपेक्षित खून बह रहा से बचने के लिए ध्यान से किया जाना चाहिए। उच्च सटीकता के साथ उच्च गुणवत्ता वाले बहुप?...

Multispectral फैलाना और परावर्तन इमेजिंग cortical ऊतकों में आंतरिक ऑप्टिकल संकेतों (IOSs) की एक स्थानिक नक्शा प्राप्त करने के लिए सबसे आम तकनीक है। IOSs इन विवो ब्रेन में मनाया मुख्य रूप से तीन घटनाएं के लिए जिम्मेदार ठहराया जाता है: रूपात्मक परिवर्तन से प्रेरित प्रकाश बिखरने गुण में प्रकाश अवशोषण में बदलाव और cortical hemodynamics के कारण बिखरने गुण, कमी या माइटोकांड्रिया में cytochromes की oxidization के आधार पर अवशोषण में बदलाव, और बदलाव 1। निकट अवरक्त (NIR) वर्णक्रम सीमा को दिखाई (तुलना) में लाइट प्रभावी रूप से अवशोषित और जैविक ऊतक द्वारा बिखरे हुए है। इन विवो ब्रेन की फैलाना और परावर्तन स्पेक्ट्रम अवशोषण और बिखरने स्पेक्ट्रा की विशेषता है। कम बिखरने गुणांक एक नीरस बिखरने स्पेक्ट्रम प्रदर्शनी में तुलना करने के लिए NIR तरंगदैर्ध्य रेंज परिणाम में मस्तिष्क के ऊतकों की s &#39;μअब तरंगदैर्य पर छोटे परिमाण ing। कम बिखरने गुणांक स्पेक्ट्रम μ s &#39;(λ) बिजली कानून समारोह 2, 3 के रूप में हो के रूप में μ एस अनुमान लगाया जा सकता&#39; (λ) = एक × λ बी। बिखरने बिजली ख ऊतक 2, 3 में रहने वाले जैविक scatterers के आकार से संबंधित है। ऊतक और cortical जीवित ऊतक की व्यवहार्यता की कमी की रूपात्मक परिवर्तन जैविक scatterers 4, 5, 6, 7, 8, 9 के आकार को प्रभावित कर सकते हैं। multispectral फैलाना और परावर्तन इमेजिंग के लिए एक ऑप्टिकल प्रणाली आसानी से एक तापदीप्त ली से निर्माण किया जा सकताGHT स्रोत, सरल ऑप्टिकल घटकों, और एक एक रंग का आरोप लगाया युग्मित डिवाइस (सीसीडी)। इसलिए, विभिन्न एल्गोरिदम और multispectral फैलाना और परावर्तन इमेजिंग के लिए ऑप्टिकल प्रणाली cortical hemodynamics और / या ऊतक आकृति विज्ञान 10, 11, 12, 13, 14, 15, 16, 17, 18 का मूल्यांकन किया गया है। विधि इस आलेख में वर्णित दोनों hemodynamics और प्रकाश बिखरने एक पारंपरिक multispectral फैलाना और परावर्तन इमेजिंग प्रणाली का उपयोग कर विवो में चूहे के मस्तिष्क के ऊतकों के गुणों कल्पना करने के लिए प्रयोग किया जाता है। वैकल्पिक तकनीकों पर इस विधि के फायदे दोनों मस्तिष्क hemodynamics और cortical ऊतकों में spatiotemporal परिवर्तन मूल्यांकन करने की क्षमता हैंआकृति विज्ञान, साथ ही विभिन्न मस्तिष्क रोग पशु मॉडल के लिए अपने प्रयोज्यता। इसलिए, विधि अभिघातजन्य मस्तिष्क चोट, मिर्गी का दौरा, स्ट्रोक, और ischemia की जांच के लिए उचित होगा।

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	<colgroup>
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		<col />
	</colgroup>
	<tbody>
		<tr height="36">
			<td height="36" style="height:36px;width:289px;">150-W halogen-lamp light source</td>
			<td style="width:281px;">Hayashi Watch Works Co., Ltd, Tokyo, Japan</td>
			<td style="width:119px;">LA-150SAE</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="36">
			<td height="36" style="height:36px;">Light guide</td>
			<td style="width:281px;">Hayashi Watch Works Co., Ltd, Tokyo, Japan</td>
			<td>LGC1-5L1000</td>
			<td></td>
		</tr>
		<tr height="36">
			<td height="36" style="height:36px;">Collecting lens</td>
			<td style="width:281px;">Hayashi Watch Works Co., Ltd, Tokyo, Japan</td>
			<td>SH-F16</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@500nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65088</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@520nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65093</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@540nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65096</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@560nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#67766</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@570nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#67767</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@580nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65646</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@600nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65102</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@730nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65115</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@760nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#67777</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Motorized filter wheel&#160;</td>
			<td>Andover Corporation, NH, USA</td>
			<td>FW-MOT-12.5</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">16-bit cooled CCD camera</td>
			<td>Bitran, Japan</td>
			<td>BS-40</td>
			<td></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;">Video zoom lens</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>VZMTM300i</td>
			<td></td>
		</tr>
		<tr height="36">
			<td height="36" style="height:36px;width:289px;">Spectralon white standard with 99% diffuse reflectance</td>
			<td>Labsphere Incorporated, North Sutton, NH, USA</td>
			<td>SRS-99-020</td>
			<td></td>
		</tr>
	</tbody>
</table>

simultaneous evaluation of cerebral hemodynamics and light scattering properties of the in vivo rat brain using multispectral diffuse reflectance imaging

The simultaneous evaluation of cerebral hemodynamics and the light scattering properties of in vivo rat brain tissue is demonstrated using a conventional multispectral diffuse reflectance imaging system. This system is constructed from a broadband white light source, a motorized filter wheel with a set of narrowband interference filters, a light guide, a collecting lens, a video zoom lens, and a monochromatic charged-coupled device (CCD) camera. An ellipsoidal cranial window is made in the skull bone of a rat under isoflurane anesthesia to capture in vivo multispectral diffuse reflectance images of the cortical surface. Regulation of the fraction of inspired oxygen using a gas mixture device enables the induction of different respiratory states such as normoxia, hyperoxia, and anoxia. A Monte Carlo simulation-based multiple regression analysis for the measured multispectral diffuse reflectance images at nine wavelengths (500, 520, 540, 560, 570, 580, 600, 730, and 760 nm) is then performed to visualize the two-dimensional maps of hemodynamics and the light scattering properties of the in vivo rat brain.

विवो चूहे के दिमाग में से प्राप्त फैलाना प्रतिबिंब की प्रतिनिधि वर्णक्रमीय छवियों को चित्रा 3 में दिखाया गया है । 500, 520, 540, 560, 570, और 580 एनएम पर छवियां स्पष्ट रूप से मस्तिष्क प्...

Watch this Scientific Journal Video about Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging at J

Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging

मस्तिष्क hemodynamics और इन विवो चूहे के मस्तिष्क के ऊतकों के प्रकाश बिखरने गुण का एक साथ मूल्यांकन एक पारंपरिक multispectral फैलाना और परावर्तन इमेजिंग प्रणाली का उपयोग कर प्रदर्शित किया गया है।

सेरेब्रल hemodynamics और के प्रकाश बिखराव गुण के एक साथ मूल्यांकन<em&gt; में विवो</em&gt; चूहा मस्तिष्क multispectral फैलाना reflectance इमेजिंग का उपयोग करना

The simultaneous evaluation of cerebral hemodynamics and the light scattering properties of in vivo rat brain tissue is demonstrated using a conventional ...

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Neuroscience

Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging

Focal Cerebral Ischemia Model by Endovascular Suture Occlusion of the Middle Cerebral Artery in the Rat

चूहा में मध्य प्रमस्तिष्क धमनी की Endovascular सीवन आड़ द्वारा फोकल सेरेब्रल Ischemia मॉडल

Stroke is the leading cause of disability and the third leading cause of death in adults worldwide1. In human stroke, there exists a highly variable ...

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI

कार्यात्मक न्यूरोइमेजिंग अल्ट्रासोनिक रक्त मस्तिष्क बाधा विघटन और मैंगनीज बढ़ाकर एमआरआई का उपयोग

Although mice are the dominant model system for studying the genetic and molecular underpinnings of neuroscience, functional neuroimaging in mice remains ...

Functional Imaging of Auditory Cortex in Adult Cats using High-field fMRI

उच्च क्षेत्र fMRI का उपयोग वयस्क बिल्लियों में श्रवण प्रांतस्था के कार्यात्मक इमेजिंग

Current knowledge of sensory processing in the mammalian auditory system is mainly derived from electrophysiological studies in a variety of animal ...

Deep Brain Stimulation with Simultaneous fMRI in Rodents

कृंतक में एक साथ fMRI साथ डीप ब्रेन उसकाव

In order to visualize the global and downstream neuronal responses to deep brain stimulation (DBS) at various targets, we have developed a protocol for ...

Simultaneous ex vivo Functional Testing of Two Retinas by in vivo Electroretinogram System

Simultaneous ex vivo Functional Testing of Two Retinas by in vivo Electroretinogram System

समकालिक<em&gt; पूर्व vivo</emदो Retinas की&gt; कार्यात्मक परीक्षण से<em&gt; Vivo</em&gt; Electroretinogram सिस्टम

An In vivo electroretinogram (ERG) signal is composed of several overlapping components originating from different retinal cell types, as well as noise ...

Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex

Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex

एक साथ दो-फोटान<em&gt; Vivo</em&gt; माउस Retrosplenial कोर्टेक्स में अन्तर्ग्रथनी आदानों की इमेजिंग और postsynaptic लक्ष्य

This video shows the craniotomy procedure that allows chronic imaging of neurons in the mouse retrosplenial cortex (RSC) using in vivo two-photon ...

Evaluation of Bioenergetic Function in Cerebral Vascular Endothelial Cells

मस्तिष्क संवहनी endothelial कोशिकाओं में bioenergetic समारोह के मूल्यांकन

The integrity of the blood-brain-barrier (BBB) is critical to prevent brain injury. Cerebral vascular endothelial (CVE) cells are one of the cell types ...

Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System

इमेजिंग एक 2D संस्कृति प्रणाली का उपयोग कर प्राथमिक मस्तिष्क प्रांतस्था की कोशिकाओं के रहते

During cerebral cortex development, progenitor cells undergo several rounds of symmetric and asymmetric cell divisions to generate new progenitors or ...

Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb

फैलाना चिंतनशील स्पेक्ट्रोस्कोपी: एक अंगूठे के तहत केशिका रिफिल परीक्षण हो रही है

The capillary refill test was introduced in 1947 to help estimate circulatory status in critically ill patients. Guidelines commonly state that refill ...

Intra-Arterial Delivery of Neural Stem Cells to the Rat and Mouse Brain: Application to Cerebral Ischemia

चूहा और माउस मस्तिष्क के लिए तंत्रिका स्टेम सेल की अंतर-धमनी वितरण: सेरेब्रल इस्केमिया के लिए आवेदन

Neural stem cell (NSC) therapy is an emerging innovative treatment for stroke, traumatic brain injury and neurodegenerative disorders. As compared to ...