Name: simultaneous evaluation of cerebral hemodynamics and light scattering properties of the in vivo rat brain using multispectral diffuse reflectance imaging
Uploaded: 2017-05-07T15:00:00
Description: Watch this Scientific Journal Video about Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging at J

Part of this work was supported by a Grant-in-Aid for Scientific Research (C) from the Japanese Society for the Promotion of Science (25350520, 22500401, 15K06105) and the US-ARMY ITC-PAC Research and Development Project (FA5209-15-P-0175).

動物のケア、準備、および実験プロトコルは、東京農工大学の動物研究委員会によって承認されました。この方法のために、ラットは、食物および水を自由に摂取して、制御された環境（24℃、12時間の明/暗サイクル）に収容されています。 従来のマルチスペクトル拡散反射イメージングシステムの構築1。 <ol><li>マウントの中心波長を有する9つの狭帯域?...

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このプロトコルの中で最も重要なステップは、頭蓋窓を作るために薄くなった頭蓋骨領域の除去です。これは予期せぬ出血を避けるために、慎重に行われるべきです。この工程は、高い精度で反射率画像を拡散多重スペクトルの高品質を得るために重要です。可能な場合は実体顕微鏡の使用は、外科的処置のために推奨されます。ゼラチンスポンジの小片は、止血のために便利です。 <p cl...

マルチスペクトル拡散反射率イメージングは​​、皮質組織に固有の光信号（IOSs）の空間的マップを取得するための最も一般的な技術です。形態学的変化によって誘導される光散乱性の光吸収の変化とによる皮質血行動態の散乱特性、低減又はミトコンドリアにおけるシトクロムの酸化に依存して吸収の変化、および変形：IOSsは、主に3つの現象に起因するインビボ脳で観察され1。 近赤外（NIR）スペクトル範囲（VIS）可視光の光を効果的に吸収され、生体組織によって散乱されます。 インビボで脳の拡散反射スペクトルは、吸収および散乱スペクトルによって特徴づけられます。換算散乱係数は単調散乱スペクトルの展示にVIS-に-NIR波長範囲の結果で脳組織のs &#39; を μより長い波長で小さい大きさをINGの。等価散乱係数μスペクトルS「（λ）は、μSなどのべき乗則関数2、3の形態であると近似することができる」（λ）×λ-bを =。散乱電力Bは、組織2,3生体内生物学的散乱体の大きさに関連しています。組織及び皮質生体組織の生存率の減少の形態学的変化は、生物学的散乱体4、5、6、7、8、9の大きさに影響を与えることができます。 マルチスペクトル拡散反射結像光学系は、容易白熱リチウムから構築することができますGHTソース、簡単な光学部品、及びモノクロ電荷結合素子（CCD）。したがって、様々なアルゴリズムおよびマルチスペクトル拡散反射撮像のための光学系が皮質血行動態および/または組織形態10、11、12、13、14、15、16、17、18を評価するために使用されてきました。 この資料に記載された方法は、血行動態及び従来のマルチスペクトル拡散反射イメージングシステムを用いて、in vivoでのラット脳組織の光散乱特性の両方を可視化するために使用されます。代替技術に比べて、この方法の利点は、脳の血行動態および皮質組織の両方における時空間変化を評価する能力です形態学だけでなく、様々な脳機能障害の動物モデルへの適用。したがって、この方法は、外傷性脳損傷、てんかん発作、脳卒中、および虚血の調査のために適切であろう。

<table border="0" cellpadding="0" cellspacing="0" width="856">
	<colgroup>
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		<col />
		<col />
		<col />
	</colgroup>
	<tbody>
		<tr height="36">
			<td height="36" style="height:36px;width:289px;">150-W halogen-lamp light source</td>
			<td style="width:281px;">Hayashi Watch Works Co., Ltd, Tokyo, Japan</td>
			<td style="width:119px;">LA-150SAE</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="36">
			<td height="36" style="height:36px;">Light guide</td>
			<td style="width:281px;">Hayashi Watch Works Co., Ltd, Tokyo, Japan</td>
			<td>LGC1-5L1000</td>
			<td></td>
		</tr>
		<tr height="36">
			<td height="36" style="height:36px;">Collecting lens</td>
			<td style="width:281px;">Hayashi Watch Works Co., Ltd, Tokyo, Japan</td>
			<td>SH-F16</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@500nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65088</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@520nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65093</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@540nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65096</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@560nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#67766</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@570nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#67767</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@580nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65646</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@600nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65102</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@730nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65115</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@760nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#67777</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Motorized filter wheel&#160;</td>
			<td>Andover Corporation, NH, USA</td>
			<td>FW-MOT-12.5</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">16-bit cooled CCD camera</td>
			<td>Bitran, Japan</td>
			<td>BS-40</td>
			<td></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;">Video zoom lens</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>VZMTM300i</td>
			<td></td>
		</tr>
		<tr height="36">
			<td height="36" style="height:36px;width:289px;">Spectralon white standard with 99% diffuse reflectance</td>
			<td>Labsphere Incorporated, North Sutton, NH, USA</td>
			<td>SRS-99-020</td>
			<td></td>
		</tr>
	</tbody>
</table>

simultaneous evaluation of cerebral hemodynamics and light scattering properties of the in vivo rat brain using multispectral diffuse reflectance imaging

The simultaneous evaluation of cerebral hemodynamics and the light scattering properties of in vivo rat brain tissue is demonstrated using a conventional multispectral diffuse reflectance imaging system. This system is constructed from a broadband white light source, a motorized filter wheel with a set of narrowband interference filters, a light guide, a collecting lens, a video zoom lens, and a monochromatic charged-coupled device (CCD) camera. An ellipsoidal cranial window is made in the skull bone of a rat under isoflurane anesthesia to capture in vivo multispectral diffuse reflectance images of the cortical surface. Regulation of the fraction of inspired oxygen using a gas mixture device enables the induction of different respiratory states such as normoxia, hyperoxia, and anoxia. A Monte Carlo simulation-based multiple regression analysis for the measured multispectral diffuse reflectance images at nine wavelengths (500, 520, 540, 560, 570, 580, 600, 730, and 760 nm) is then performed to visualize the two-dimensional maps of hemodynamics and the light scattering properties of the in vivo rat brain.

インビボラットの脳から得られた拡散反射の代表的なスペクトル画像を図3に示す.500,520,540,560,570,580nmの画像は、大脳皮質の血管の緻密なネットワークを明瞭に視覚化する。 600,730,760nmの画像で観察される血管と周囲組織との間のコントラストの低下は、より長い波長およびNIR波長でのヘモグロビンによる光のより低い吸収を反映する。 <p class="jo...

Watch this Scientific Journal Video about Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging at J

Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging

脳血行動態およびin vivoでのラット脳組織の光散乱特性の同時評価は、従来のマルチスペクトル拡散反射イメージングシステムを用いて実証されています。

脳血行動態との光散乱特性の同時評価<em&gt;インビボ</emマルチスペクトル拡散反射率イメージングの使用&gt;ラット脳

The simultaneous evaluation of cerebral hemodynamics and the light scattering properties of in vivo rat brain tissue is demonstrated using a conventional ...

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Neuroscience

Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging

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同時<em&gt; ex vivoで</emによる二つの網膜の&gt;機能テスト<em&gt; in vivoで</em&gt;電図システム

An In vivo electroretinogram (ERG) signal is composed of several overlapping components originating from different retinal cell types, as well as noise ...

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同時2光子<em&gt;インビボ</emマウス脳梁膨大後部皮質におけるシナプス入力とシナプス後ターゲットの&gt;イメージング

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The integrity of the blood-brain-barrier (BBB) is critical to prevent brain injury. Cerebral vascular endothelial (CVE) cells are one of the cell types ...

Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System

2次元培養系を用いた主な大脳皮質細胞のイメージングをライブします。

During cerebral cortex development, progenitor cells undergo several rounds of symmetric and asymmetric cell divisions to generate new progenitors or ...

Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb

拡散反射率分光: 取得 1 つの親指の下の毛細血管リフィル テスト

The capillary refill test was introduced in 1947 to help estimate circulatory status in critically ill patients. Guidelines commonly state that refill ...

Intra-Arterial Delivery of Neural Stem Cells to the Rat and Mouse Brain: Application to Cerebral Ischemia

ラットおよびマウス脳への神経幹細胞の動脈内デリバリー:脳虚血への応用

Neural stem cell (NSC) therapy is an emerging innovative treatment for stroke, traumatic brain injury and neurodegenerative disorders. As compared to ...