Name: simultaneous evaluation of cerebral hemodynamics and light scattering properties of the in vivo rat brain using multispectral diffuse reflectance imaging
Uploaded: 2017-05-07T15:00:00
Description: Watch this Scientific Journal Video about Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging at J

Part of this work was supported by a Grant-in-Aid for Scientific Research (C) from the Japanese Society for the Promotion of Science (25350520, 22500401, 15K06105) and the US-ARMY ITC-PAC Research and Development Project (FA5209-15-P-0175).

Dyrpleie, forberedelse og eksperimentelle protokoller ble godkjent av dyreforskningsutvalget i Tokyo University of Agriculture and Technology. For denne metoden er roten innkapslet i et kontrollert miljø (24 ° C, 12 h lys / mørk syklus), med mat og vann tilgjengelig ad libitum. 1. Konstruksjon av et konvensjonelt multispectral diffus reflekterings-imaging system <ol><li> Monter ni smalbånd optiske interferensfiltre med senterbølgelengder på 500, 520, 540, 560, 570, 580, 600,...

<ol>
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Den mest kritiske trinn i denne protokollen er fjerning av den fortynnede skallen området for å gjøre cranial vinduet; dette bør gjøres nøye for å unngå uventet blødning. Dette trinn er viktig for å oppnå høy kvalitet multispektrale diffuse reflektansmålinger bilder med høy nøyaktighet. Bruken av en stereo anbefales for den kirurgiske prosedyren hvis mulig. Små biter av gelatin svamp er nyttige for hemostase. Det optiske system som er beskrevet i denne artikkelen passerer en ...

Multispektrale diffus refleksjon avbildning er den mest vanlige teknikk for å oppnå et kart over romlig iboende optiske signaler (IOSs) i kortikalt vev. IOSs observert i in vivo hjernen er i hovedsak knyttet til tre fenomener: variasjoner i lys absorpsjon og spredning egenskaper på grunn av cortical hemodynamikk, variasjon i absorpsjon avhengig av reduksjon eller oksidasjon av cytokromer i mitokondrier, og variasjoner i lysspredningsegenskaper indusert av morfologiske endringer 1. Lys i det synlige (VIS) til nær-infrarødt (NIR) spektralområdet absorberes effektivt og spres av biologisk vev. Den diffuse reflektansspektrum av in vivo hjernen er karakterisert ved absorpsjon og spredning spektra. De reduserte spredningskoeffisienter jj s &#39;av hjernevev i VIS-til-NIR-bølgelengdeområdet resultat i en monoton spredning spektrum oppvisering mindre størrelser ved lengre bølgelengder. Den reduserte spredningskoeffisienten spektrum μ s &#39;(λ) kan tilnærmes til å være i form av kraftlovindeksen funksjon 2, 3 som μ s&#39; (λ) = a x λ -b. Den spredende kraft b er relatert til størrelsen av biologiske spredningsobjekter i levende vev 2, 3. Morfologiske forandringer av vev og reduksjon av levedyktigheten av levende kortikalt vev kan påvirke størrelsen av de biologiske spredeflater 4, 5, 6, 7, 8, 9. Et optisk system for multispektrale diffuse refleksjonsavbildning kan lett konstrueres fra en gløde liGHT kilde, enkle optiske komponenter, og en monokromatisk ladet koblet innretning (CCD). Derfor har forskjellige algoritmer og optiske systemer for multispektrale diffuse refleksjonsavbildning blitt anvendt for å vurdere kortikale hemodynamikk og / eller vev morfologi 10, 11, 12, 13, 14, 15, 16, 17, 18. Metoden som beskrives i denne artikkelen blir brukt til å visualisere både hemodynamikk og lysspredende egenskapene til rotte cerebral vev in vivo ved hjelp av en konvensjonell multispektrale diffus reflektans avbildningssystem. Fordelene med denne fremgangsmåten fremfor alternative teknikker er evnen til å vurdere endringer i tid og rom både cerebrale hemodynamikk og kortikalt vevmorfologi, så vel som dens anvendelighet for forskjellige hjernedysfunksjon dyremodeller. Derfor vil fremgangsmåten være egnet for undersøkelser av traumatisk hjerneskade, epileptiske anfall, slag og iskemi.

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		<tr height="36">
			<td height="36" style="height:36px;width:289px;">150-W halogen-lamp light source</td>
			<td style="width:281px;">Hayashi Watch Works Co., Ltd, Tokyo, Japan</td>
			<td style="width:119px;">LA-150SAE</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="36">
			<td height="36" style="height:36px;">Light guide</td>
			<td style="width:281px;">Hayashi Watch Works Co., Ltd, Tokyo, Japan</td>
			<td>LGC1-5L1000</td>
			<td></td>
		</tr>
		<tr height="36">
			<td height="36" style="height:36px;">Collecting lens</td>
			<td style="width:281px;">Hayashi Watch Works Co., Ltd, Tokyo, Japan</td>
			<td>SH-F16</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@500nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65088</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@520nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65093</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@540nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65096</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@560nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#67766</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@570nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#67767</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@580nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65646</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@600nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65102</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@730nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65115</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@760nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#67777</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Motorized filter wheel&#160;</td>
			<td>Andover Corporation, NH, USA</td>
			<td>FW-MOT-12.5</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">16-bit cooled CCD camera</td>
			<td>Bitran, Japan</td>
			<td>BS-40</td>
			<td></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;">Video zoom lens</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>VZMTM300i</td>
			<td></td>
		</tr>
		<tr height="36">
			<td height="36" style="height:36px;width:289px;">Spectralon white standard with 99% diffuse reflectance</td>
			<td>Labsphere Incorporated, North Sutton, NH, USA</td>
			<td>SRS-99-020</td>
			<td></td>
		</tr>
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simultaneous evaluation of cerebral hemodynamics and light scattering properties of the in vivo rat brain using multispectral diffuse reflectance imaging

The simultaneous evaluation of cerebral hemodynamics and the light scattering properties of in vivo rat brain tissue is demonstrated using a conventional multispectral diffuse reflectance imaging system. This system is constructed from a broadband white light source, a motorized filter wheel with a set of narrowband interference filters, a light guide, a collecting lens, a video zoom lens, and a monochromatic charged-coupled device (CCD) camera. An ellipsoidal cranial window is made in the skull bone of a rat under isoflurane anesthesia to capture in vivo multispectral diffuse reflectance images of the cortical surface. Regulation of the fraction of inspired oxygen using a gas mixture device enables the induction of different respiratory states such as normoxia, hyperoxia, and anoxia. A Monte Carlo simulation-based multiple regression analysis for the measured multispectral diffuse reflectance images at nine wavelengths (500, 520, 540, 560, 570, 580, 600, 730, and 760 nm) is then performed to visualize the two-dimensional maps of hemodynamics and the light scattering properties of the in vivo rat brain.

Representative spektralbilder av diffus reflektans ervervet fra in vivo-rottehjerner er vist i figur 3. Bildene ved 500, 520, 540, 560, 570, og 580 nm tydelig se et tett nettverk av blodkar i den cerebrale cortex. Forringelse av kontrasten mellom blodkar og det omgivende vev observert i bildene ved 600, 730 og 760 nm reflekterer den nedre absorpsjon av lys av hemoglobin ved lengre og NIR-bølgelengder. <p class="jove_content" fo:keep-together.within-page="1"...

Watch this Scientific Journal Video about Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging at J

Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging

Den samtidige evaluering av cerebrale hemodynamikk og den lysspredende egenskapene til in vivo rottehjernevevet er vist ved bruk av en konvensjonell multispektrale diffus reflektans avbildningssystem.

Samtidig Evaluering av Cerebral hemodynamikken og lysspredning egenskaper av<em&gt; In vivo</em&gt; Rat Brain Bruke multispektrale Diffus refleksjon Imaging

The simultaneous evaluation of cerebral hemodynamics and the light scattering properties of in vivo rat brain tissue is demonstrated using a conventional ...

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Neuroscience

Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging

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