Name: simultaneous evaluation of cerebral hemodynamics and light scattering properties of the in vivo rat brain using multispectral diffuse reflectance imaging
Uploaded: 2017-05-07T15:00:00
Description: Watch this Scientific Journal Video about Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging at J

Part of this work was supported by a Grant-in-Aid for Scientific Research (C) from the Japanese Society for the Promotion of Science (25350520, 22500401, 15K06105) and the US-ARMY ITC-PAC Research and Development Project (FA5209-15-P-0175).

Djurvård, förberedelse och experimentella protokoll godkändes av kommittén Animal Research of Tokyo University of Agriculture and Technology. För denna metodik, är råttan inrymt i en kontrollerad miljö (24 ° C, 12 h ljus / mörker-cykel), med mat och vatten tillgängligt ad libitum. 1. Konstruktion av en konventionell Multispektrala Diffus Reflektans Imaging System <ol><li> Mount nio smalbandiga optiska interferensfilter med centrumvåglängder av 500, 520, 540, 560, 570,...

<ol>
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Det mest kritiska steget i detta protokoll är avlägsnandet av den förtunnade skallen regionen för att göra den kraniala fönstret; Detta bör utföras noggrant för att undvika oväntade blödningar. Detta steg är viktigt för att få hög kvalitet multispektrala diffus reflektans bilder med hög noggrannhet. Användningen av ett stereomikroskop rekommenderas för kirurgiska ingrepp om det är möjligt. Små bitar av gelatinsvamp är användbara för hemostas. Det optiska system som be...

Multispektral diffus reflektans avbildning är den vanligaste tekniken för att erhålla en rumslig karta över karaktäristiska optiska signaler (IOSs) i kortikal vävnad. IOSs observerats i in vivo hjärnan är huvudsakligen hänföras till tre fenomen: Avvikelser i Ijusabsorption och spridningsegenskaper på grund av kortikala hemodynamik, variation i absorption beroende på minskning eller oxidation av cytokromer i mitokondrier, och variationer i ljusspridande egenskaper inducerade av morfologiska förändringar 1. Ljus i det synliga (VIS) till nära infraröda (NIR) spektralområdet absorberas effektivt och sprids av biologisk vävnad. Den diffus reflektans-spektrum för den in vivo hjärnan kännetecknas av absorption och spridning spektra. De reducerade spridningskoefficienterna | j s &#39;av hjärnvävnad i våglängdsområdet resultat VIS-till-NIR i en monoton spridningsspektrum uppvisarMed mindre magnituder vid längre våglängder. Det reducerade spridningskoefficientspektrumet μs &#39;(λ) kan approximeras för att vara i form av effektlagsfunktionen 2 , 3 som μ s &#39; (A) = a × X- b . Spridningskraften b är relaterad till storleken av biologiska spridare i levande vävnad 2 , 3 . Morfologiska förändringar av vävnaden och reduktion av livskraften hos levande kortikalvävnad kan påverka storleken hos de biologiska spriddarna 4 , 5 , 6 , 7 , 8 , 9 . Ett optiskt system för multispektral diffus reflektansavbildning kan lätt konstrueras från en glödlampa light källa, enkla optiska komponenter, och en monokromatisk laddningskopplad anordning (CCD). Därför har olika algoritmer och optiska system för multispektral diffus reflektans avbildning använts för att utvärdera kortikala hemodynamik och / eller vävnad morfologi 10, 11, 12, 13, 14, 15, 16, 17, 18. Den metod som beskrivs i denna artikel används för att visualisera både hemodynamiken och ljusspridande egenskaper hos råtta cerebral vävnad in vivo med användning av ett konventionellt reflektans avbildningssystem multispektral diffus. Fördelarna med denna metod jämfört med alternativa tekniker är förmågan att utvärdera Spatiotemporal förändringar i båda hjärn hemodynamik och kortikal vävnadmorfologi, liksom dess användbarhet till olika hjärndysfunktion djurmodeller. Därför kommer metoden vara lämplig för undersökningar av traumatisk hjärnskada, epileptiskt anfall, stroke och ischemi.

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		<col />
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		<col />
	</colgroup>
	<tbody>
		<tr height="36">
			<td height="36" style="height:36px;width:289px;">150-W halogen-lamp light source</td>
			<td style="width:281px;">Hayashi Watch Works Co., Ltd, Tokyo, Japan</td>
			<td style="width:119px;">LA-150SAE</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="36">
			<td height="36" style="height:36px;">Light guide</td>
			<td style="width:281px;">Hayashi Watch Works Co., Ltd, Tokyo, Japan</td>
			<td>LGC1-5L1000</td>
			<td></td>
		</tr>
		<tr height="36">
			<td height="36" style="height:36px;">Collecting lens</td>
			<td style="width:281px;">Hayashi Watch Works Co., Ltd, Tokyo, Japan</td>
			<td>SH-F16</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@500nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65088</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@520nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65093</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@540nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65096</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@560nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#67766</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@570nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#67767</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@580nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65646</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@600nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65102</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@730nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#65115</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Interference filters l@760nm</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>#67777</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Motorized filter wheel&#160;</td>
			<td>Andover Corporation, NH, USA</td>
			<td>FW-MOT-12.5</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">16-bit cooled CCD camera</td>
			<td>Bitran, Japan</td>
			<td>BS-40</td>
			<td></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;">Video zoom lens</td>
			<td>Edmund Optics Japan Ltd, Tokyo, Japan</td>
			<td>VZMTM300i</td>
			<td></td>
		</tr>
		<tr height="36">
			<td height="36" style="height:36px;width:289px;">Spectralon white standard with 99% diffuse reflectance</td>
			<td>Labsphere Incorporated, North Sutton, NH, USA</td>
			<td>SRS-99-020</td>
			<td></td>
		</tr>
	</tbody>
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simultaneous evaluation of cerebral hemodynamics and light scattering properties of the in vivo rat brain using multispectral diffuse reflectance imaging

The simultaneous evaluation of cerebral hemodynamics and the light scattering properties of in vivo rat brain tissue is demonstrated using a conventional multispectral diffuse reflectance imaging system. This system is constructed from a broadband white light source, a motorized filter wheel with a set of narrowband interference filters, a light guide, a collecting lens, a video zoom lens, and a monochromatic charged-coupled device (CCD) camera. An ellipsoidal cranial window is made in the skull bone of a rat under isoflurane anesthesia to capture in vivo multispectral diffuse reflectance images of the cortical surface. Regulation of the fraction of inspired oxygen using a gas mixture device enables the induction of different respiratory states such as normoxia, hyperoxia, and anoxia. A Monte Carlo simulation-based multiple regression analysis for the measured multispectral diffuse reflectance images at nine wavelengths (500, 520, 540, 560, 570, 580, 600, 730, and 760 nm) is then performed to visualize the two-dimensional maps of hemodynamics and the light scattering properties of the in vivo rat brain.

Representativa spektrala bilder av diffus reflektans förvärvats från in vivo råtthjärnor visas i Figur 3. Bilderna vid 500, 520, 540, 560, 570, och 580 nm tydligt visualisera ett tätt nätverk av blodkärl i hjärnbarken. Försämringen av kontrasten mellan blodkärl och omgivande vävnad observeras i bilderna vid 600, 730 och 760 nm återspeglar den lägre absorptionen av ljus genom hemoglobin vid längre och NIR våglängder. <p class="jove_content" ...

Watch this Scientific Journal Video about Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging at J

Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging

Den samtidiga utvärderingen av cerebrala hemodynamiken och de ljusspridande egenskaperna hos in vivo råtthjärnvävnad demonstreras med användning av en konventionell reflektans avbildningssystem multispektral diffus.

Samtidig utvärdering av cerebral Hemodynamik och ljusspridande egenskaper hos<em&gt; In Vivo</em&gt; Rat Brain Använda Multispektrala diffus reflektans Imaging

The simultaneous evaluation of cerebral hemodynamics and the light scattering properties of in vivo rat brain tissue is demonstrated using a conventional ...

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Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging

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