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DOI :
10.3791/55403-v
March 7th, 2017
Chapters
0:05
Title
0:58
Preparation of Agarose-coated Plates
3:06
Cell Culture and Multicellular Tumor Spheroid Formation
6:17
Medium Exchange Using a Robotic System
7:14
High-content Imaging of MCTS and Semi-automated Image Analysis
8:04
Analysis of Multicellular Spheroid Formation in the Presence of Anti-cancer Drugs
10:16
Conclusion
マイクロタイタープレートからの培地の不均一な損失は、均一な多細胞腫瘍スフェロイド形成の再現性に影響を与えます。重要な媒体の損失を低減するために培養条件を改善するスフェロイド形成の再現性及び液体オーバーレイ技術を使用して回転楕円体に基づくアッセイの結果を改善します。
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