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DOI :
10.3791/55465-v
April 5th, 2017
Chapters
0:05
Title
0:51
Lung Harvest and Preparation
2:36
Lung Slicing
4:29
Static PCLS Imaging
5:44
PCLS Live-cell Imaging
7:01
Results: Representative Immune Cell Population Identification
8:36
Conclusion
我们描述了用于产生精密切割肺片(PCLS)和免疫它们以显现各种免疫细胞类型的定位在肺中的方法。我们的协议可以扩展在各种条件下可视化的位置和许多不同细胞类型的功能。
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