Name: experimental protocol of a three-minute, all-out arm crank exercise test in spinal-cord injured and able-bodied individuals
Uploaded: 2017-06-08T13:00:00
Description: Watch this Scientific Journal Video about Experimental Protocol of a Three-minute, All-out Arm Crank Exercise Test in Spinal-cord Injured and Able-bodied Individuals at JoVE.com

我们非常感谢Martina Lienert和Fabienne Schaufelberger在运动测试期间的帮助，以及PD Claudio Perret博士的科学建议。

该研究得到当地伦理委员会（Ethikkommission Nordwest- und Zentralschweiz，Basel，Switzerland）的批准，并在开始研究之前获得了与会者的书面知情同意书。 测试准备和参与者指导<ol><li> 手臂曲柄测力计 <ol><li>在打开软件之前，打开旋转速度相关的手臂曲柄测力计的电源。 </li><li>选择测试方案3分钟，全能测力计测试。 <ol><li>插入一个新的协议，120秒的预热，180秒的测试时?...

<ol>
	<li>Conconi, F., Ferrari, M., Ziglio, P. G., Droghetti, P., Codeca, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Determination+of+the+anaerobic+threshold+by+a+noninvasive+field+test+in+runners.">Determination of the anaerobic threshold by a noninvasive field test in runners.</a> J Appl Physiol. 52 (4), 869-873 (1982).</li><li>Strupler, M., Mueller, G., Perret, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Heart+rate-based+lactate+minimum+test:+a+reproducible+method.">Heart rate-based lactate minimum test: a reproducible method.</a> Br J Sports Med. 43 (6), 432-436 (2009).</li><li>Black, M. I., Durant, J., Jones, A. M., Vanhatalo, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Critical+power+derived+from+a+3-min+all-out+test+predicts+16.1-km+road+time-trial+performance.">Critical power derived from a 3-min all-out test predicts 16.1-km road time-trial performance.</a> Eur J Sport Sci. 14 (3), 217-223 (2014).</li><li>Burnley, M., Doust, J. H., Vanhatalo, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+3-min+all-out+test+to+determine+peak+oxygen+uptake+and+the+maximal+steady+state.">A 3-min all-out test to determine peak oxygen uptake and the maximal steady state.</a> Med Sci Sports Exerc. 38 (11), 1995-2003 (2006).</li><li>Vanhatalo, A., Doust, J. H., Burnley, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+3-min+all-out+cycling+test+is+sensitive+to+a+change+in+critical+power.">A 3-min all-out cycling test is sensitive to a change in critical power.</a> Med Sci Sports Exerc. 40 (9), 1693-1699 (2008).</li><li>Johnson, T. M., Sexton, P. J., Placek, A. M., Murray, S. R., Pettitt, R. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reliability+analysis+of+the+3-min+all-out+exercise+test+for+cycle+ergometry.">Reliability analysis of the 3-min all-out exercise test for cycle ergometry.</a> Med Sci Sports Exerc. 43 (12), 2375-2380 (2011).</li><li>Cheng, C. F., Yang, Y. S., Lin, H. M., Lee, C. L., Wang, C. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Determination+of+critical+power+in+trained+rowers+using+a+three-minute+all-out+rowing+test.">Determination of critical power in trained rowers using a three-minute all-out rowing test.</a> Eur J Appl Physiol. 112 (4), 1251-1260 (2012).</li><li>Fukuda, D. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterization+of+the+work-time+relationship+during+cross-country+ski+ergometry.">Characterization of the work-time relationship during cross-country ski ergometry.</a> Physiol Meas. 35 (1), 31-43 (2014).</li><li>Vanhatalo, A., McNaughton, L. R., Siegler, J., Jones, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+induced+alkalosis+on+the+power-duration+relationship+of+"all-out"+exercise.">Effect of induced alkalosis on the power-duration relationship of "all-out" exercise.</a> Med. Sci. Sports Exerc. 42 (3), 563-570 (2010).</li><li>Jacobs, P. L., Johnson, B., Somarriba, G. A., Carter, A. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reliability+of+upper+extremity+anaerobic+power+assessment+in+persons+with+tetraplegia.">Reliability of upper extremity anaerobic power assessment in persons with tetraplegia.</a> J Spinal Cord Med. 28 (2), 109-113 (2005).</li><li>Jacobs, P. L., Mahoney, E. T., Johnson, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reliability+of+arm+Wingate+Anaerobic+Testing+in+persons+with+complete+paraplegia.">Reliability of arm Wingate Anaerobic Testing in persons with complete paraplegia.</a> J Spinal Cord Med. 26 (2), 141-144 (2003).</li><li>Flueck, J. L., Lienert, M., Schaufelberger, F., Perret, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reliability+of+a+3-min+all-out+arm+crank+ergometer+exercise+test.">Reliability of a 3-min all-out arm crank ergometer exercise test.</a> Int J Sports Med. 36 (10), 809-813 (2015).</li><li>Erich Jaeger GmbH. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> User Manual Oxycon Pro. , (2016).</li><li>Shrout, P. E., Fleiss, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intraclass+correlations:+uses+in+assessing+rater+reliability.">Intraclass correlations: uses in assessing rater reliability.</a> Psychol Bull. 86 (2), 420-428 (1979).</li><li>Beckerman, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Smallest+real+difference,+a+link+between+reproducibility+and+responsiveness.">Smallest real difference, a link between reproducibility and responsiveness.</a> Qual Life Res. 10 (7), 571-578 (2001).</li><li>Plichta, S. B., Kelvin, E. A., Munro, B. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Munro's statistical methods for health care research. , (2011).</li><li>Atkinson, G., Nevill, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Statistical+methods+for+assessing+measurement+error+(reliability)+in+variables+relevant+to+sports+medicine.">Statistical methods for assessing measurement error (reliability) in variables relevant to sports medicine.</a> Sports Med. 26 (4), 217-238 (1998).</li><li>Bland, J. M., Altman, D. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Measuring+agreement+in+method+comparison+studies.">Measuring agreement in method comparison studies.</a> Stat Methods Med Res. 8 (2), 135-160 (1999).</li><li>van Drongelen, S., Maas, J. C., Scheel-Sailer, A., Van Der Woude, L. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Submaximal+arm+crank+ergometry:+Effects+of+crank+axis+positioning+on+mechanical+efficiency,+physiological+strain+and+perceived+discomfort.">Submaximal arm crank ergometry: Effects of crank axis positioning on mechanical efficiency, physiological strain and perceived discomfort.</a> J. Med. Eng. Technol. 33 (2), 151-157 (2009).</li><li>Bressel, E., Bressel, M., Marquez, M., Heise, G. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+effect+of+handgrip+position+on+upper+extremity+neuromuscular+responses+to+arm+cranking+exercise.">The effect of handgrip position on upper extremity neuromuscular responses to arm cranking exercise.</a> J. Electromyogr. Kinesiol. 11 (4), 291-298 (2001).</li><li>West, C. R., Goosey-Tolfrey, V. L., Campbell, I. G., Romer, L. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+abdominal+binding+on+respiratory+mechanics+during+exercise+in+athletes+with+cervical+spinal+cord+injury.">Effect of abdominal binding on respiratory mechanics during exercise in athletes with cervical spinal cord injury.</a> J Appl Physiol (1985). 117 (1), 36-45 (2014).</li><li>West, C. R., Campbell, I. G., Goosey-Tolfrey, V. L., Mason, B. S., Romer, L. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+abdominal+binding+on+field-based+exercise+responses+in+Paralympic+athletes+with+cervical+spinal+cord+injury.">Effects of abdominal binding on field-based exercise responses in Paralympic athletes with cervical spinal cord injury.</a> J. Sci. Med. Sport. 17 (4), 351-355 (2014).</li><li>Kirshblum, S. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=International+standards+for+neurological+classification+of+spinal+cord+injury.">International standards for neurological classification of spinal cord injury.</a> J Spinal Cord Med. 34 (6), 535-546 (2011).</li></ol>

脊髓损伤运动员的运动测试对于追踪几个月或几年训练中的运动表现至关重要。只有几个运动测试存在，以检查手臂曲柄测力计的短期，高强度运动表现。该方法详细描述了已经检查其在骑自行车5和划艇7中的可靠性的运动测试可以应用于手臂曲柄测力计。为了收集可靠而有意义的成果，有两个因素非常重要：一是为参与者准备本次运动测试，二是进行标准化?...

有几项运动测试可准确地确定精英运动员在训练期间1,2,3,4,5的运动表现的增加。这些测试中的一个是在制动的自行车测力计3,4,5,6上可靠的3分钟全程运动测试。该测试用于确定临界功率，但也适用于运动员的运动测试，以及研究7,8,9 。由于该测试主要用于下肢性能，如在划艇7和骑自行车3，5 ，类似t需要用于上身运动的估计方案。主要使用上身的运动学科除了体育运动员或体力较弱的个体（ 例如截肢或由于脊髓损伤引起的肢体损伤）之外，也可能是这样一种新的测试方案的受益者。因此，手臂曲柄测力计上的测试方案是一个很好的工具，可轻松测试来自不同体育学科的各种运动员的上身运动表现。 存在一个非常相似的30年代Wingate手臂曲柄测力计测试10,11帮助制定了一个3分钟，全面的手臂曲柄测力计测试。其持续时间与1500米轮椅比赛非常相似。因此，这种新的测试方案为3分钟，全功能臂曲柄测力计测试测试其重测信度12 。总体而言，thi的可靠性s测试协议非常好，因此它可能是上身运动测试领域的未来测试工具。然而，使用这种运动测试需要注意，特别是在测试患有脊髓损伤的个体时。因此，本实验文章的目的是展示一个详细的方案，不仅描述了测试设置和测试结果的分析，而且还显示了测试能力强的人员和脊髓损伤运动员之间的差异。

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Angio V2 arm crank ergometer</td>
			<td>Lode BV, Groningen, NL</td>
			<td>N/A</td>
			<td>arm crank ergometer</td>
		</tr>
		<tr>
			<td>Lode Ergometry Manager Software</td>
			<td>Lode BV, Groningen, NL</td>
			<td>N/A</td>
			<td>Software</td>
		</tr>
		<tr>
			<td>10ul end-to-end capillary</td>
			<td>EKF-diagnostics GmbH, Barleben, Germany</td>
			<td>0209-0100-005</td>
			<td>Capillaries</td>
		</tr>
		<tr>
			<td>haemolysis cup</td>
			<td>EKF-diagnostics GmbH, Barleben, Germany</td>
			<td>0209-0100-006</td>
			<td>hemolysis cup</td>
		</tr>
		<tr>
			<td>lactate analyzer</td>
			<td>Biosen C line, EKF-diagnostics GmbH</td>
			<td>5213-0051-6200</td>
			<td>lactate analyzer</td>
		</tr>
		<tr>
			<td>Heart rate monitor, Polar 610i</td>
			<td>Polar, Kempele, Finland</td>
			<td>P610i</td>
			<td>heart rate monitor</td>
		</tr>
		<tr>
			<td>metabolic cart, Oxygen Pro</td>
			<td>Jaeger GmbH</td>
			<td>N/A</td>
			<td>metabolic cart</td>
		</tr>
		<tr>
			<td>oxygen mask, Hans Rudolph</td>
			<td>Hans Rudolph Inc. , USA</td>
			<td>113814</td>
			<td>oxygen mask</td>
		</tr>
		<tr>
			<td>statistical software, PSAW Software</td>
			<td>SPSS Inc., Chicago USA</td>
			<td>N/A</td>
			<td>statistical software</td>
		</tr>
		<tr>
			<td>desinfectant, Soft-Zellin</td>
			<td>Hartmann GmbH, Austria</td>
			<td>999979</td>
			<td>desinfectant</td>
		</tr>
		<tr>
			<td>Quality control cup, EasyCon Norm</td>
			<td>EKF-diagnostics GmbH, Barleben, Germany</td>
			<td>0201-005.012P6</td>
			<td>quality control</td>
		</tr>
		<tr>
			<td>Quality control cup 3mmol/L</td>
			<td>EKF-diagnostics GmbH, Barleben, Germany</td>
			<td>5130-6152</td>
			<td>control cup</td>
		</tr>
		<tr>
			<td>Chip sensor lactate analyzer</td>
			<td>EKF-diagnostics GmbH, Barleben, Germany</td>
			<td>5206-3029</td>
			<td>chip sensor</td>
		</tr>
		<tr>
			<td>Lactate system solution</td>
			<td>EKF-diagnostics GmbH, Barleben, Germany</td>
			<td>0201-0002-025</td>
			<td>lactate system solution</td>
		</tr>
		<tr>
			<td>lancet, Mediware Blutlanzetten</td>
			<td>medilab</td>
			<td>54041</td>
			<td>lancet</td>
		</tr>
		<tr>
			<td>Calibration gas,&#160;</td>
			<td>Jaeger GmbH</td>
			<td>36-MC G020</td>
			<td>calibration gas</td>
		</tr>
		<tr>
			<td>chair provided by distributor (ergoselect)</td>
			<td>ergoline GmbH, Germany</td>
			<td>N/A</td>
			<td>chair provided by distributor</td>
		</tr>
	</tbody>
</table>

experimental protocol of a three-minute, all-out arm crank exercise test in spinal-cord injured and able-bodied individuals

需要可靠的运动方案来测试精英运动员运动表现的变化。这些运动员的表现改善可能很小;因此，敏感工具是锻炼生理的基础。目前，有许多运动测试可以检测运动能力强壮的运动员，主要用于下肢或全身运动。有一种趋势是测试运动员在体育特定的环境中非常类似于参与者习惯执行的动作。只有少数协议测试参与者的短期，高强度运动能力，对身体有损害。大多数这些协议是非常体育专用的，不适用于广泛的运动员。一个众所周知的测试方案是30秒的Wingate测试，它在骑自行车和手臂曲柄运动测试中已经建立起来。该测试在30秒内分析高强度运动表现ñ。为了在更长的时间内监测运动表现，修改了适用于上身的不同方法。 3分钟，全面的手臂曲柄测力计测试允许运动员以特定的1500米轮椅赛跑（运动时间）的方式进行测试，以及上身锻炼，如划船或手动循环。为了在相同的测试条件下提高可靠性，重要的是精确地复制设置，例如电阻（ 即扭矩因子）和参与者的位置（ 即曲柄的高度，曲柄和曲柄之间的距离参与者和参与者的固定）。另一个重要的问题是运动测试的开始。需要固定每分钟的转数来标准运动测试开始时的测试条件。此运动协议显示准确操作重现相同测试条件和设置的重要性。

在21名休闲训练（但不具体为上身训练），非吸烟个体（男9例，女12例，年龄34±11岁，体重69.6±11.1 kg，身高： 175.5±6.9厘米）。 表1显示了相对和绝对测试重新测试可靠性的结果12 。测试和重新测试之间的峰值功率如图 12所示 。 图2 12给出了该测试重新测试的Bland Altman图。然后，将...

Watch this Scientific Journal Video about Experimental Protocol of a Three-minute, All-out Arm Crank Exercise Test in Spinal-cord Injured and Able-bodied Individuals at JoVE.com

Experimental Protocol of a Three-minute, All-out Arm Crank Exercise Test in Spinal-cord Injured and Able-bodied Individuals

我们提出一个方案，测试上肢肌肉的有氧和无氧能力，持续时间为3分钟，身体健康，截瘫和四肢个体。该方案在具有或不具有残疾的个体中对其适用于上肢运动的具体修改。

脊髓损伤和健康人员三分钟全能臂曲柄运动试验实验方案

Reliable exercise protocols are required to test changes in exercise performance in elite athletes. Performance improvements in these athletes may be ...

Research

JoVE Journal

Medicine

Acute and Chronic Tactile Sensory Testing after Spinal Cord Injury in Rats

在大鼠脊髓损伤后的急性和慢性触觉测试

Spinal cord injury (SCI) impairs sensory systems causing allodynia1-8. To identify cellular and molecular causes of allodynia, sensitive and valid sensory ...

科研

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A Contusion Model of Severe Spinal Cord Injury in Rats

挫伤模型脊髓严重损伤的保护作用

The translational potential of novel treatments should be investigated in severe spinal cord injury (SCI) contusion models. A detailed methodology is ...

Evaluation of Respiratory Muscle Activation Using Respiratory Motor Control Assessment (RMCA) in Individuals with Chronic Spinal Cord Injury

Evaluation of Respiratory Muscle Activation Using Respiratory Motor Control Assessment RMCA in Individuals with Chronic Spinal Cord Injury

评价呼吸肌慢性脊髓损伤个人使用呼吸电机控制评估（RMCA）

During breathing, activation of respiratory muscles is coordinated by integrated input from the brain, brainstem, and spinal cord. When this coordination ...

Implanting Glass Spinal Cord Windows in Adult Mice with Experimental Autoimmune Encephalomyelitis

玻璃植入脊髓的Windows在成年小鼠实验性自身免疫性脑脊髓炎

Experimental autoimmune encephalomyelitis (EAE) in adult rodents is the standard experimental model for studying autonomic demyelinating diseases such as ...

Controlled Cortical Impact Model for Traumatic Brain Injury

控制皮层影响模型创伤性脑损伤

Every year over a million Americans suffer a traumatic brain injury (TBI).  Combined with the incidence of TBIs worldwide, the physical, emotional, ...

A Radio-telemetric System to Monitor Cardiovascular Function in Rats with Spinal Cord Transection and Embryonic Neural Stem Cell Grafts

无线电遥测系统，以监测大鼠脊髓横断和胚胎神经干细胞移植在心血管功能

High thoracic or cervical spinal cord injury (SCI) can lead to cardiovascular dysfunction. To monitor cardiovascular parameters, we implanted a catheter ...

Synergetic Use of Neural Precursor Cells and Self-assembling Peptides in Experimental Cervical Spinal Cord Injury

在实验颈脊髓损伤协同使用神经前体细胞，并自组装肽的

Spinal cord injuries (SCI) cause serious neurological impairment and psychological, economic, and social consequences for patients and their families. ...

Neural Stem Cell Transplantation in Experimental Contusive Model of Spinal Cord Injury

在脊髓损伤的实验挫伤型号神经干细胞移植

Spinal cord injury is a devastating clinical condition, characterized by a complex of neurological dysfunctions. Animal models of spinal cord injury can ...

Contrast Enhanced Ultrasound Imaging for Assessment of Spinal Cord Blood Flow in Experimental Spinal Cord Injury

超声造影成像脊髓血流量的评估实验脊髓损伤

Reduced spinal cord blood flow (SCBF) (i.e., ischemia) plays a key role in traumatic spinal cord injury (SCI) pathophysiology and is accordingly an ...

Determining The Electromyographic Fatigue Threshold Following a Single Visit Exercise Test

确定肌电疲劳阈值以下单次访问运动试验

Theoretically, the electromyographic (EMG) fatigue threshold is the exercise intensity an individual can maintain indefinitely without the need to recruit ...