Name: experimental protocol of a three-minute, all-out arm crank exercise test in spinal-cord injured and able-bodied individuals
Uploaded: 2017-06-08T13:00:00
Description: Watch this Scientific Journal Video about Experimental Protocol of a Three-minute, All-out Arm Crank Exercise Test in Spinal-cord Injured and Able-bodied Individuals at JoVE.com

Vi er taknemmelige for hjælpen fra Martina Lienert og Fabienne Schaufelberger under træningstest samt fra PD Claudio Perret, ph.d. for hans videnskabelige rådgivning.

Undersøgelsen blev godkendt af det lokale etiske udvalg (Ethikkommission Nordwest- und Zentralschweiz, Basel, Schweiz), og der blev indhentet skriftligt informeret samtykke fra deltagerne inden undersøgelsen startede. 1. Test forberedelse og deltager instruktion <ol><li> Armkrums ergometer <ol><li> Slå strømmen til det drejningsmomentafhængige armkrums ergometer, inden du åbner softwaren. </li><li> Vælg testprotokol til 3 min, all-out ergometer testen. <ol><li> Inds...

<ol>
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M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+abdominal+binding+on+respiratory+mechanics+during+exercise+in+athletes+with+cervical+spinal+cord+injury.">Effect of abdominal binding on respiratory mechanics during exercise in athletes with cervical spinal cord injury.</a> J Appl Physiol (1985). 117 (1), 36-45 (2014).</li><li>West, C. R., Campbell, I. G., Goosey-Tolfrey, V. L., Mason, B. S., Romer, L. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+abdominal+binding+on+field-based+exercise+responses+in+Paralympic+athletes+with+cervical+spinal+cord+injury.">Effects of abdominal binding on field-based exercise responses in Paralympic athletes with cervical spinal cord injury.</a> J. Sci. Med. Sport. 17 (4), 351-355 (2014).</li><li>Kirshblum, S. 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Træningstest i rygmarvsskadede atleter er afgørende for at følge træningsevnen i løbet af flere måneder eller mange års træning. Der findes kun nogle få øvelsestests for at kontrollere kortvarig, højintensiv træningsevne på armkrumtegometeret. Denne metode beskriver detaljeret, hvordan en øvelsestest, der allerede blev undersøgt for dens pålidelighed i cykling 5 og roing 7, kunne anvendes på armkrumtegometeret. For at indsamle pålidelige og meningsfulde r...

Der er flere øvelsestests, der præcist bestemmer stigningen i træningsevnen hos elite atleter i løbet af en træningsperiode 1 , 2 , 3 , 4 , 5 . En af disse tests er den pålidelige 3- mins all-out øvelsestest på et bremset cykel ergometer 3 , 4 , 5 , 6 . Denne test blev brugt til at bestemme kritisk kraft, men det blev også anvendt til øvelse test med atleter samt at undersøge 7 , 8 , 9 . Da denne test hovedsagelig blev anvendt til ydre ydre ydelser, såsom ved roning 7 og cykling 3 , 5 , en lignende tEsting protokol til overkrop øvelse var nødvendig. Sportsdiscipliner, der primært bruger overkroppen, kan være mulige modtagere af en ny testprotokol, ud over atleter eller individer med nedsat påvirkning af underkroppens muskler ( fx en amputation eller en forringelse af lemmer på grund af rygmarvsskade). Derfor er en testprotokol på armkrumtegometeret et godt værktøj til nemt at teste træning i overkroppen hos en række atleter fra forskellige sportsdiscipliner. Eksistensen af ​​en meget lignende 30 s Wingate armkrummometer ergometer test 10 , 11 hjalp med udviklingen af ​​en protokol for en 3 min, all-out arm crank ergometer test. Dens varighed ligner meget på en 1500 kørestolsbane. Derfor blev denne nye testprotokol af 3 min, all-out arm crank ergometer testen testet for sin test-retest pålidelighed 12 . Samlet set er pålideligheden af ​​denneS testprotokol var fremragende, så det kunne være et fremtidigt testværktøj inden for øvelsen i øvelsen. Ikke desto mindre kræver brugen af ​​denne øvelsestest opmærksomhed, især når man tester personer med rygmarvsskade. Formålet med denne eksperimentelle artikel er derfor at demonstrere en detaljeret protokol, der beskriver ikke kun testindstillingerne og analysen af ​​testresultaterne, men det viser også forskellene mellem testning af fysiske personer og atleter med rygmarvsskade.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Angio V2 arm crank ergometer</td>
			<td>Lode BV, Groningen, NL</td>
			<td>N/A</td>
			<td>arm crank ergometer</td>
		</tr>
		<tr>
			<td>Lode Ergometry Manager Software</td>
			<td>Lode BV, Groningen, NL</td>
			<td>N/A</td>
			<td>Software</td>
		</tr>
		<tr>
			<td>10ul end-to-end capillary</td>
			<td>EKF-diagnostics GmbH, Barleben, Germany</td>
			<td>0209-0100-005</td>
			<td>Capillaries</td>
		</tr>
		<tr>
			<td>haemolysis cup</td>
			<td>EKF-diagnostics GmbH, Barleben, Germany</td>
			<td>0209-0100-006</td>
			<td>hemolysis cup</td>
		</tr>
		<tr>
			<td>lactate analyzer</td>
			<td>Biosen C line, EKF-diagnostics GmbH</td>
			<td>5213-0051-6200</td>
			<td>lactate analyzer</td>
		</tr>
		<tr>
			<td>Heart rate monitor, Polar 610i</td>
			<td>Polar, Kempele, Finland</td>
			<td>P610i</td>
			<td>heart rate monitor</td>
		</tr>
		<tr>
			<td>metabolic cart, Oxygen Pro</td>
			<td>Jaeger GmbH</td>
			<td>N/A</td>
			<td>metabolic cart</td>
		</tr>
		<tr>
			<td>oxygen mask, Hans Rudolph</td>
			<td>Hans Rudolph Inc. , USA</td>
			<td>113814</td>
			<td>oxygen mask</td>
		</tr>
		<tr>
			<td>statistical software, PSAW Software</td>
			<td>SPSS Inc., Chicago USA</td>
			<td>N/A</td>
			<td>statistical software</td>
		</tr>
		<tr>
			<td>desinfectant, Soft-Zellin</td>
			<td>Hartmann GmbH, Austria</td>
			<td>999979</td>
			<td>desinfectant</td>
		</tr>
		<tr>
			<td>Quality control cup, EasyCon Norm</td>
			<td>EKF-diagnostics GmbH, Barleben, Germany</td>
			<td>0201-005.012P6</td>
			<td>quality control</td>
		</tr>
		<tr>
			<td>Quality control cup 3mmol/L</td>
			<td>EKF-diagnostics GmbH, Barleben, Germany</td>
			<td>5130-6152</td>
			<td>control cup</td>
		</tr>
		<tr>
			<td>Chip sensor lactate analyzer</td>
			<td>EKF-diagnostics GmbH, Barleben, Germany</td>
			<td>5206-3029</td>
			<td>chip sensor</td>
		</tr>
		<tr>
			<td>Lactate system solution</td>
			<td>EKF-diagnostics GmbH, Barleben, Germany</td>
			<td>0201-0002-025</td>
			<td>lactate system solution</td>
		</tr>
		<tr>
			<td>lancet, Mediware Blutlanzetten</td>
			<td>medilab</td>
			<td>54041</td>
			<td>lancet</td>
		</tr>
		<tr>
			<td>Calibration gas,&#160;</td>
			<td>Jaeger GmbH</td>
			<td>36-MC G020</td>
			<td>calibration gas</td>
		</tr>
		<tr>
			<td>chair provided by distributor (ergoselect)</td>
			<td>ergoline GmbH, Germany</td>
			<td>N/A</td>
			<td>chair provided by distributor</td>
		</tr>
	</tbody>
</table>

experimental protocol of a three-minute, all-out arm crank exercise test in spinal-cord injured and able-bodied individuals

Pålidelige øvelsesprotokoller er nødvendige for at afprøve ændringer i træningsevne hos elite atleter. Ydeevne forbedringer i disse atleter kan være små; Derfor er følsomme værktøjer grundlæggende for at udøve fysiologi. Der er i øjeblikket mange øvelsesprøver, der gør det muligt at undersøge træningskapaciteten hos veluddannede atleter, med protokoller hovedsagelig til underkrops- eller helkropsøvelse. Der er en tendens til at teste atleter i en sportsspecifik indstilling, som ligner de handlinger, som deltagerne er vant til at udføre. Kun få protokoller tester kortvarig, højintensiv træningskapacitet hos deltagere med nedsat underkrop. De fleste af disse protokoller er meget sportsspecifikke og gælder ikke for en bred vifte af atleter. En velkendt testprotokol er 30 s Wingate-testen, som er veletableret i cykling og i armkrumtræningstestning. Denne test analyserer høj intensitetsøvelse i løbet af 30 s tid duration. For at overvåge træningsevnen over længere tid blev en anden metode ændret til applikation på overkroppen. Den 3 min. All-out arm crank ergometer test tillader atleter at blive testet på en måde, der er specifik for 1.500 m kørestole (med hensyn til træningsvarighed), såvel som øvelser i overkroppen som rodd eller håndcykling. For at øge pålideligheden med identiske testbetingelser er det afgørende at præcist replikere indstillinger som modstanden ( dvs. drejningsmomentfaktoren) og deltagernes position ( dvs. højden af ​​vevet, afstanden mellem vev og vinkel Deltager og fastgørelse af deltageren). Et andet vigtigt spørgsmål vedrører starten af ​​øvelsen. Der kræves faste omdrejninger pr. Minut for at standardisere testbetingelserne for træningstestens start. Denne øvelsesprotokol viser vigtigheden af ​​nøjagtige operationer for at gengive identiske testbetingelser og indstillinger.

Test-retest pålideligheden blev kontrolleret i 21 rekreativt trænet (men ikke specifikt overkroppen uddannet), ikke-ryger personer (9 mænd, 12 kvinder, alder: 34 ± 11 år, kropsmasse: 69,6 ± 11,1 kg og højde: 175,5 ± 6,9 cm). Tabel 1 viser resultaterne for de relative og absolutte test-retest reliabiliteter 12 . Den maksimale effekt sammenlignet mellem testen og retest er vist i figur 1 12 . Et Bland...

Watch this Scientific Journal Video about Experimental Protocol of a Three-minute, All-out Arm Crank Exercise Test in Spinal-cord Injured and Able-bodied Individuals at JoVE.com

Experimental Protocol of a Three-minute, All-out Arm Crank Exercise Test in Spinal-cord Injured and Able-bodied Individuals

Vi præsenterer en protokol til at teste den aerobic og anaerobe kraft i overkroppens muskler over en varighed på 3 minutter i ubevægelige såvel som i paraplegiske og tetraplegiske individer. Protokollen præsenterer specifikke ændringer i sin ansøgning om træning i overkroppen hos personer med eller uden handicap.

Eksperimentel protokol af en tre minutters udbredt øvelse i rygsøjlen Skadede og ufaglærte personer

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Spinal cord injury (SCI) impairs sensory systems causing allodynia1-8. To identify cellular and molecular causes of allodynia, sensitive and valid sensory ...

A Contusion Model of Severe Spinal Cord Injury in Rats

En Kontusion Model of Svær Rygmarvsskader i rotter

The translational potential of novel treatments should be investigated in severe spinal cord injury (SCI) contusion models. A detailed methodology is ...

Evaluation of Respiratory Muscle Activation Using Respiratory Motor Control Assessment (RMCA) in Individuals with Chronic Spinal Cord Injury

Evaluation of Respiratory Muscle Activation Using Respiratory Motor Control Assessment RMCA in Individuals with Chronic Spinal Cord Injury

Evaluering af Respiratory Muscle Activation Brug Respiratory Motor Control Assessment (RMCA) hos personer med kronisk Rygmarvsskader

During breathing, activation of respiratory muscles is coordinated by integrated input from the brain, brainstem, and spinal cord. When this coordination ...

Implanting Glass Spinal Cord Windows in Adult Mice with Experimental Autoimmune Encephalomyelitis

Implantere Glas Spinal Cord Vinduer i voksne mus med eksperimentel autoimmun encephalomyelitis

Experimental autoimmune encephalomyelitis (EAE) in adult rodents is the standard experimental model for studying autonomic demyelinating diseases such as ...

Controlled Cortical Impact Model for Traumatic Brain Injury

Kontrolleret Cortical Impact Model for Traumatisk Hjerneskade

Every year over a million Americans suffer a traumatic brain injury (TBI).  Combined with the incidence of TBIs worldwide, the physical, emotional, ...

A Radio-telemetric System to Monitor Cardiovascular Function in Rats with Spinal Cord Transection and Embryonic Neural Stem Cell Grafts

En radio-telemetri system til overvågning Kardiovaskulær funktion i rotter med Spinal Cord transection og embryonale neural stamcelle transplantater

High thoracic or cervical spinal cord injury (SCI) can lead to cardiovascular dysfunction. To monitor cardiovascular parameters, we implanted a catheter ...

Synergetic Use of Neural Precursor Cells and Self-assembling Peptides in Experimental Cervical Spinal Cord Injury

Fordelagtig samordning af neurale precursorceller og selvsamlende peptider i Experimental Cervikal Rygmarvsskader

Spinal cord injuries (SCI) cause serious neurological impairment and psychological, economic, and social consequences for patients and their families. ...

Neural Stem Cell Transplantation in Experimental Contusive Model of Spinal Cord Injury

Neural stamcelletransplantation i Eksperimentel Contusive Model for Rygmarvsskader

Spinal cord injury is a devastating clinical condition, characterized by a complex of neurological dysfunctions. Animal models of spinal cord injury can ...

Contrast Enhanced Ultrasound Imaging for Assessment of Spinal Cord Blood Flow in Experimental Spinal Cord Injury

Kontrast Forbedret Ultralydbilleddannelse for Vurdering af Spinal Cord Blood Flow i Experimental Rygmarvsskader

Reduced spinal cord blood flow (SCBF) (i.e., ischemia) plays a key role in traumatic spinal cord injury (SCI) pathophysiology and is accordingly an ...

Determining The Electromyographic Fatigue Threshold Following a Single Visit Exercise Test

Bestemmelse af Elektromyografisk Træthed Threshold Efter et enkelt besøg Motion Test

Theoretically, the electromyographic (EMG) fatigue threshold is the exercise intensity an individual can maintain indefinitely without the need to recruit ...