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DOI :
10.3791/55496-v
March 18th, 2017
Chapters
0:05
Title
0:54
Lyse Bacterial Pellets Expressing Human Recombinant G4 Resolvase1 (rG4R1)
3:06
Prepare G4-magnetic Beads
4:07
Bind Histidine-tagged rG4R1 to Cobalt Beads
5:47
Elute rG4R1 from Cobalt Beads
7:07
Bind rG4R1 to G4-bound SPB and Elute in an ATP-dependent Manner
8:55
Results: Analysis and Quantification of Catalytically Active, Purified rG4R1
10:06
Conclusion
G4 Resolvase1结合G-四(G4)的结构与一个G4结合蛋白的紧密报道的亲和力,并表示在HeLa细胞中的大多数G4-DNA解旋活动。我们描述了线束的亲和力和G4-Resolvase1的ATP依赖的平仓活动,特别是净化催化活性的重组G4R1一个新的协议。
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