Results: Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
0:52
Protein-DNA Sample Preparation for AFM
3:14
AFM Imaging of Protein-DNA Sample
6:37
AFM Analysis of Protein Complex Volume
Transcript
The overall goal of this atomic force microscopy experiment is to resolve interactions of DNA repair proteins with damage sites in DNA and to distinguish between different DNA interactions by various protein complexes. This method can help answer
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Here, the study of different DNA lesion recognition approaches via single molecule AFM imaging is demonstrated with the nucleotide excision repair system as an example. The procedures of DNA and protein sample preparations and experimental as well as analytical details for the AFM experiments are described.