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DOI :
10.3791/55519-v
April 14th, 2017
Chapters
0:05
Title
0:57
Dissecting the Larval Ring Gland-brain Complex
1:53
Filleting Larvae to Maintain Relative Tissue Positions
3:35
Fine Dissection of the Brain-ring Gland Complex and Mounting
5:34
Dissection of Adult Ovary
6:36
Dissection of Adult Reproductive Organs Along with Other Interactive Tissues
8:15
Mounting of Adult Structures and Imaging
9:33
Results: Preparation images
11:13
Conclusion
我们描述了解剖,固定,并在果蝇幼虫和成年女性类固醇合成器官,研究类固醇激素的生物合成及其调控机制的免疫染色的协议。除了类固醇合成器官,我们可以形象地类固醇合成器官的神经支配和类固醇合成的靶细胞,如生殖系干细胞。
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