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DOI :
10.3791/55526-v
June 25th, 2017
Chapters
0:05
Title
1:22
CAPRRESI Oligonucleotide Design
7:18
Conclusion
6:43
Results: Example Sequence of Assembled Gene
3:29
Plasmid Disruption by PCR Followed by Restriction Enzyme Digestion
5:15
Plasmid Recovery Using DNA Ligation and Bacterial Transformation
在这里,我们通过质粒恢复和限制性酶切位点插入(CAPRRESI)提供嵌合体装配,这是基于将限制酶位点插入同义词DNA序列和功能质粒恢复的方案。该协议是一种快速和低成本的融合蛋白质编码基因的方法。
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