Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/55526-v
June 25th, 2017
Chapters
0:05
Title
1:22
CAPRRESI Oligonucleotide Design
7:18
Conclusion
6:43
Results: Example Sequence of Assembled Gene
3:29
Plasmid Disruption by PCR Followed by Restriction Enzyme Digestion
5:15
Plasmid Recovery Using DNA Ligation and Bacterial Transformation
ここでは、同義語DNA配列への制限酵素部位の挿入および機能的プラスミド回収に基づくプロトコルであるプラスミド回収および制限酵素部位挿入(CAPRRESI)によるキメラアセンブリを提示する。このプロトコールは、タンパク質コード遺伝子を融合するための迅速かつ低コストの方法である。
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved