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DOI :
10.3791/55576-v
May 16th, 2017
Chapters
0:05
Title
10:31
Conclusion
8:33
Results: Purification, Characterization, and Crystallization of rBC1531
0:55
Recombinant Protein Production and Purification of rBC1531
4:47
Removal of Affinity Tags by Thrombin Proteolysis
6:32
Crystallization Screening and Optimization of rBC1531
7:30
Colorimetric NTPase Assay
炭疽芽孢杆菌是致命性吸入性炭疽的专性病原体,因此对其基因和蛋白质的研究受到严格管制。另一种方法是研究直系同源基因。我们描述了蜡状芽孢杆菌Bc1531中的炭疽杆菌直系同源物的生物物理化学研究,需要最少的实验设备和缺乏严重的安全性问题。
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