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DOI :
10.3791/55576-v
May 16th, 2017
Chapters
0:05
Title
10:31
Conclusion
8:33
Results: Purification, Characterization, and Crystallization of rBC1531
0:55
Recombinant Protein Production and Purification of rBC1531
4:47
Removal of Affinity Tags by Thrombin Proteolysis
6:32
Crystallization Screening and Optimization of rBC1531
7:30
Colorimetric NTPase Assay
Bacillus anthracisは致命的な吸入炭疽菌の必須病原体であるため、その遺伝子やタンパク質の研究は厳格に規制されています。代わりのアプローチは、オーソロガス遺伝子を研究することである。最小の実験装置を必要とし、重大な安全性の懸念がないB.セレウスの B. anthracisオルソログ(bc1531)の生物物理化学的研究を記載する。
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