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DOI :
10.3791/55585-v
April 17th, 2017
Chapters
0:05
Title
0:37
Preparation of Media for Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC)
1:34
SILAC-labeling and Amino Acid Incorporation Control
3:15
Lipid Droplet Isolation of SILAC-labeled Huh7.5 Cells
7:59
Analysis of Lipid Droplets Purity
8:53
Results: Qualitative and Quantitative Analysis of the Lipid Droplets
9:57
Conclusion
脂肪滴は、C型肝炎ウイルス(HCV)を含むいくつかの病原体の複製のための重要な器官です。我々は、関連するタンパク質の定量的な質量分析のための脂質滴を単離する方法を記載します。そのようなウイルス感染、環境ストレス、または薬物治療などの条件の様々な環境下で使用することができます。
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