Name: a technique for subcutaneous abdominal adipose tissue biopsy via a non-diathermy method
Uploaded: 2017-09-30T13:00:00
Description: Watch this Scientific Journal Video about A Technique for Subcutaneous Abdominal Adipose Tissue Biopsy via a Non-diathermy Method at JoVE.com

这项工作得到了欧洲联盟2020期 (赠款协议 645710) 和第七框架 (赠款协议612547和 319010) 方案的资助。作者希望感谢 Dr. Fotini Sourli-Chasioti 对活检程序的帮助。作者还感谢 Mr. Yiorgos Paterakis 在视频制作方面的宝贵帮助。

该研究符合《赫尔辛基宣言》规定的标准, 并获得萨利大学、运动科学系、#914; 我和 #959; 道德委员会. 
 1. 手术设备和消耗品的准备 <ol> <li> 将一个消毒的外科手术场放置在一个4轮手术的梅奥站上. </li>
	<li> 将下列耗材和手术设备放置在4轮手术的梅奥站上: 
	一双直操作剪刀 (15 厘米) 一手术刀 (No. 11) 一手术刀柄 一双弯曲剪刀 (14 厘米) 一只蚊子钳 ?...

<ol>
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本研究的目的是规范一项手术技术的皮下腹部脂肪组织活检, 进行局部麻醉下进行了 non-diathermy 方法的健康男性。透在手术室里常用;但是, 可能出现的副作用 (即,闪火和环境毒性)9和愈合中的延迟10 。透的这些有害影响会给操作人员和患者/参与者带来健康风险。因此, 有必要进行皮下脂肪组织活检的手术 non-diathermy 方法。
在手术后两?...

脂肪组织活检提供组织样本, 经分析后, 可以提供有关新陈代谢和疾病的机制的有见地的概述。例如, 它们用于乳腺癌肿瘤的鉴定1, 脂肪酸组成检查的2, 以及重要的代谢和荷尔蒙疾病通路的研究3。为了获得腹部区域的皮下脂肪组织活检, 研究人员和医生使用的是外科手术或 needle-based 技术。然而, 手术皮下脂肪活检可以提供组织样本, 可能提供一个更全面的概述复杂的生物机制 [例如,信使核糖核酸 (mRNA) 的表达, 组织学分析,等]4.它们以前已用于健康成人4 , 可在床边5中安全地执行。
needle-based 活检技术需要强烈的抽脂, 可能破坏组织的完整性, 它通常检索少量的组织 (100-500 毫克)6。这些组织标本可能不适合复杂的研究研究。此外, 与 needle-based 技术相比, 手术皮下脂肪活检可以提供组织样本, 可以绘制一个更完整的图片的几个炎症指数 (例如, b-和 T 细胞信号);细胞骨架调节;细胞通路;脂质、碳水化合物和氨基酸的代谢途径;和氧化磷酸化途径4。此外, needle-based 技术包括更高的风险, 血液细胞污染比外科手术一个4, 而提供了关于组织的纤维化区域的小信息4,7,8.
通常, 手术脂肪组织活检包括透治疗烧灼血管, 以防止过多出血9。透也被用于烧伤和破坏受疾病感染的组织和疣或组织的影响9。这项技术特别适用于神经外科和眼科手术9。
过度使用透可能导致坏死组织的形成, 导致感染和延迟愈合过程10。事实上, 在透9之后, 报告的副作用如闪光火和组织中的皮肤损害。此外, 有人担心透的外科烟雾对手术环境的毒性, 这可能会被参与者/病人和操作人员吸入9。事实上, 使用透可能会导致手术室空气的毒性等于吸烟六香烟的11和, 虽然手术室通常有良好的通风系统, 污染可以持续至少20分钟12.然而, 在进行扁桃体手术的患者中, 透方法显示出较少, 但显著, 术后疼痛13和出血的14比传统的结扎止血方法。总的来说, 关于健康个体脂肪组织活检后疼痛和出血率差异的证据几乎不存在。鉴于缺乏证据和透的副作用, 有必要对脂肪组织活检的 non-diathermy 方法进行标准化。因此, 本研究的目的是规范一项手术技术的皮下腹部脂肪组织活检, 进行局部麻醉下使用 non-diathermy 法的健康男性。此外, 我们概述了组织收集的程序, 以确定 mRNA 的表达和蛋白质浓度, 并进行组织学分析。

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			<td>Bipolar, catalog number 213015</td>
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			<td height="21" style="height:21px;">Sterile surgical gloves&#160;</td>
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			<td>Digas medical equipment</td>
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			<td height="21" style="height:21px;">Liquid nitrogen at -190&#176;C</td>
			<td>Revival</td>
			<td>Not applicable</td>
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a technique for subcutaneous abdominal adipose tissue biopsy via a non-diathermy method

脂肪组织活检提供组织样本, 经分析后, 可提供有关代谢和疾病机制的有见地的概述。为了获得腹部区域的皮下脂肪组织活检, 研究人员和医生使用的是外科手术或 needle-based 技术。然而, 手术皮下脂肪活检可以提供组织样本, 可以提供更全面的概述的复杂性生物指标的白色脂肪组织。通常, 手术脂肪组织活检包括透治疗烧灼血管, 以防止过度出血。然而, 在透后, 有报道称, 在组织中出现了闪光和皮肤损伤等副作用。因此, 我们的目的是规范一个外科腹部脂肪组织活检进行局部麻醉使用 non-diathermy 方法。我们进行了115皮下脂肪组织活检的健康男性使用 non-diathermy 腹部手术活检方法。我们的结果显示, 三例术后出血过多, 115 手术 (2.61%)。总之, 我们的标准化皮下腹部脂肪组织手术活检使用 non-diathermy 方法可以安全地应用于健康的人在床边, 最小的副作用。

参与者的特征在表 1中提供。切口区域的缝线在接下来的8-12 天内脱落。我们一共完成了115皮下脂肪组织活检使用 non-diathermy 腹部外科活检方法在健康成人 (年龄: 35.2 ±6.9 年; 身体质量索引: 27.3 ± 4.4; 和身体脂肪百分比: 27.0 ± 9.0%)。术后两天观察三例 (患病率: 2.61%) 后活检过度出血。三名经历过大出血的人比没有经历过任何副作用的人年长 (t(113)=-2.115, p ...

Watch this Scientific Journal Video about A Technique for Subcutaneous Abdominal Adipose Tissue Biopsy via a Non-diathermy Method at JoVE.com

A Technique for Subcutaneous Abdominal Adipose Tissue Biopsy via a Non-diathermy Method

我们用局部麻醉下的 non-diathermy 法对腹部脂肪组织进行了活检。三例术后大出血115手术 (2.61%) 发生。我们的结论是, 腹部脂肪组织手术活检使用 non-diathermy 的方法可以安全地应用于健康男性。

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Subcutaneous Angiotensin II Infusion using Osmotic Pumps Induces Aortic Aneurysms in Mice

使用渗透皮下血管紧张素Ⅱ输液泵诱导主动脉瘤小鼠

Osmotic pumps continuously deliver compounds at a constant rate into small animals.  This article introduces a standard protocol used to induce aortic ...

Creation of Abdominal Adhesions in Mice

小鼠腹腔粘连的创建

Abdominal adhesions consist of fibrotic tissue that forms in the peritoneal space in response to an inflammatory insult, typically surgery or ...

Creation of a Rodent Model of Abdominal Aortic Aneurysm by Blocking Adventitial Vasa Vasorum Perfusion

阻断外瓦萨血管灌注对腹主动脉瘤鼠模型的建立

The adventitial vasa vasorum (VV) provides oxygen and nourishment to the aortic wall. Hypoxia in the aortic wall can cause enlarged abdominal aortic ...

Inguinal Subcutaneous White Adipose Tissue (ISWAT) Transplantation Model of Murine Islets

Inguinal Subcutaneous White Adipose Tissue ISWAT Transplantation Model of Murine Islets

毛里岛皮下白脂肪组织（ISWAT）移植模型

Pancreatic islet transplantation is a well-established therapeutic treatment for type 1 diabetes. The kidney capsule is the most commonly used site for ...