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DOI :
10.3791/55610-v
April 20th, 2017
Chapters
0:05
Title
0:38
Growing Liquid Yeast Culture
9:32
Conclusion
8:15
Results: Astral Microtubule Dynamics in Altered in tub2-430Δ Mutant Cells
3:36
Loading Yeast Cells into Prepared Flow Chamber Slides
5:03
Image Acquisition
6:30
Image Analysis
1:26
Preparing Flow Chamber Slides
出芽酵母は、その強力な遺伝学とその微小管細胞骨格のシンプルさにin vivoでの微小管ダイナミクスを研究するための有利なモデルです。以下のプロトコルを変換し、培養酵母細胞、共焦点顕微鏡画像を取得し、かつ定量的に酵母生細胞における微小管ダイナミクスを分析する方法について説明します。
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