Name: measurement of basal and forskolin-stimulated lipolysis in inguinal adipose fat pads
Uploaded: 2017-07-21T12:30:00
Description: Watch this Scientific Journal Video about Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads at JoVE.com

Bu çalışma, AHA Ödülü No. 15BGIA23250030, NIH Ulusal Tıp Bilimleri Enstitüsü, 8P20 GM103432-12 ödülü ve Wyoming Üniversitesi Teknik Destek Bursu adı altında BT tarafından desteklenmiştir.

Tüm protokoller, Wyoming Üniversitesi&#39;ndeki hayvan bakım kılavuzlarına uymaktadır. 1. Hayvan Barınağı ve Beslenme NOT: Yetişkin vahşi tipli fareler (C57BL / 6) (12-24 haftalık yaşlar), Kurumsal Hayvan Bakımı ve Kullanım Komitesi (IACUC) onaylı protokollere göre araştırma hayvan tesislerinde yetiştirilmiştir. <ol><li> Yaşın 6. haftasından itibaren ev fareleri dört grup halinde ayrı kafeste gruplandı ve rastgele onları 38 yaşına...

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	<li>Schweiger, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Measurement+of+lipolysis.">Measurement of lipolysis.</a> Methods Enzymol. 538, 171-193 (2014).</li><li>Coelho, M., Oliveira, T., Fernandes, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Biochemistry+of+adipose+tissue:+an+endocrine+organ.">Biochemistry of adipose tissue: an endocrine organ.</a> Arch Med Sci. 9 (2), 191-200 (2013).</li><li>Richard, D., Picard, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Brown+fat+biology+and+thermogenesis.">Brown fat biology and thermogenesis.</a> Front Biosci (Landmark Ed). 16, 1233-1260 (2011).</li><li>Barquissau, V., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=White-to-brite+conversion+in+human+adipocytes+promotes+metabolic+reprogramming+towards+fatty+acid+anabolic+and+catabolic+pathways.">White-to-brite conversion in human adipocytes promotes metabolic reprogramming towards fatty acid anabolic and catabolic pathways.</a> Mol Metab. 5 (5), 352-365 (2016).</li><li>Rodriguez, A., Ezquerro, S., Mendez-Gimenez, L., Becerril, S., Fruhbeck, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Revisiting+the+adipocyte:+a+model+for+integration+of+cytokine+signaling+in+the+regulation+of+energy+metabolism.">Revisiting the adipocyte: a model for integration of cytokine signaling in the regulation of energy metabolism.</a> Am J Physiol Endocrinol Metab. 309 (8), E691-E714 (2015).</li><li>Giordano, A., Smorlesi, A., Frontini, A., Barbatelli, G., Cinti, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=White,+brown+and+pink+adipocytes:+the+extraordinary+plasticity+of+the+adipose+organ.">White, brown and pink adipocytes: the extraordinary plasticity of the adipose organ.</a> Eur J Endocrinol. 170 (5), R159-R171 (2014).</li><li>Langin, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adipose+tissue+lipolysis+as+a+metabolic+pathway+to+define+pharmacological+strategies+against+obesity+and+the+metabolic+syndrome.">Adipose tissue lipolysis as a metabolic pathway to define pharmacological strategies against obesity and the metabolic syndrome.</a> Pharmacol Res. 53 (6), 482-491 (2006).</li><li>Zimmermann, R., Lass, A., Haemmerle, G., Zechner, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fate+of+fat:+the+role+of+adipose+triglyceride+lipase+in+lipolysis.">Fate of fat: the role of adipose triglyceride lipase in lipolysis.</a> Biochim Biophys Acta. 1791 (6), 494-500 (2009).</li><li>Miyoshi, H., Perfield, J. W., Obin, M. S., Greenberg, A. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adipose+triglyceride+lipase+regulates+basal+lipolysis+and+lipid+droplet+size+in+adipocytes.">Adipose triglyceride lipase regulates basal lipolysis and lipid droplet size in adipocytes.</a> J Cell Biochem. 105 (6), 1430-1436 (2008).</li><li>Kolditz, C. I., Langin, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adipose+tissue+lipolysis.">Adipose tissue lipolysis.</a> Curr Opin Clin Nutr Metab Care. 13 (4), 377-381 (2010).</li><li>Baskaran, P., Krishnan, V., Ren, J., Thyagarajan, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Capsaicin+induces+browning+of+white+adipose+tissue+and+counters+obesity+by+activating+TRPV1+channel-dependent+mechanisms.">Capsaicin induces browning of white adipose tissue and counters obesity by activating TRPV1 channel-dependent mechanisms.</a> Br J Pharmacol. 173 (15), 2369-2389 (2016).</li><li>Yang, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Activation+of+TRPV1+by+dietary+capsaicin+improves+endothelium-dependent+vasorelaxation+and+prevents+hypertension.">Activation of TRPV1 by dietary capsaicin improves endothelium-dependent vasorelaxation and prevents hypertension.</a> Cell Metab. 12 (2), 130-141 (2010).</li><li>Ding, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reduced+lipolysis+response+to+adipose+afferent+reflex+involved+in+impaired+activation+of+adrenoceptor-cAMP-PKA-hormone+sensitive+lipase+pathway+in+obesity.">Reduced lipolysis response to adipose afferent reflex involved in impaired activation of adrenoceptor-cAMP-PKA-hormone sensitive lipase pathway in obesity.</a> Sci Rep. 6, 34374 (2016).</li><li>Ohyama, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+combination+of+exercise+and+capsinoid+supplementation+additively+suppresses+diet-induced+obesity+by+increasing+energy+expenditure+in+mice.">A combination of exercise and capsinoid supplementation additively suppresses diet-induced obesity by increasing energy expenditure in mice.</a> Am J Physiol Endocrinol Metab. 308 (4), E315-E323 (2015).</li><li>Beylot, M., Martin, C., Beaufrere, B., Riou, J. 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Hormonal regulation of lipolysis in adipose tissue.</a> Am J Physiol Gastrointest Liver Physiol. 293 (1), G1-G4 (2007).</li><li>Jeppesen, J., Kiens, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regulation+and+limitations+to+fatty+acid+oxidation+during+exercise.">Regulation and limitations to fatty acid oxidation during exercise.</a> J Physiol. 590 (5), 1059-1068 (2012).</li><li>Nakamura, M. T., Yudell, B. E., Loor, J. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regulation+of+energy+metabolism+by+long-chain+fatty+acids.">Regulation of energy metabolism by long-chain fatty acids.</a> Prog Lipid Res. 53, 124-144 (2014).</li><li>Murray, A. J., Panagia, M., Hauton, D., Gibbons, G. 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TGL&#39;nin gliserol ve yağ asitleri haline dönüşümü, bazal lipoliz sırasında ATGL 9 ile katalize edilir ve uyarılan lipoliz 21 , 22 , 23 sırasında adenil siklaz / PKA&#39;ya bağımlı yolağın aktivasyonu da dahil olmak üzere bir dizi protein ile düzenlenir. Lipolizin arttırılması, ulaşım ve enerji kullanımı için yağ asitlerinin plazma seviyelerini arttırır 24...

Yağ dokuları yağ 2 olarak enerji depolarlar ve termojenez 3 , 4 için yağ asidi oksidasyonu gerekir. Diyetle yutulan yağ asitleri apoproteinler ile birlikte şilomikronlara paketlenir ve kan dolaşımıyla vücuttaki farklı dokulara aktarılır. Vücuttaki çoğu hücrenin bir enerji rezervi depolamasına rağmen, yağ dokusu yağ olarak fazla enerji depolar 5 , 6 . Yağ dokusundaki lipoliz kompleks proseslerle düzenlenir ve lipolizin moleküler detayları halen belirsiz kalmaktadır 7 . Lipoliz, yağ dokusunda depolanan trigliseridlerin (TGL), yağlı trigliserid lipaz (ATGL) 8 enzimi ile gliserol ve yağ asitleri (FA) üretmek üzere hidrolizlenmesiyle oluşan bir işlemdir. Bazal ve uyarılmış lipolizdeki değişiklikler obezitenin karakteristik bir özelliğidir. BAsal lipoliz, TGL&#39;yi diasilgliserol (DAG) haline getiren, daha sonra monoasil gliserole (MAG) hidrolize edilen ATGL aktivasyonu 9 ile düzenlenir. Adenillyla siklaz yoluyla hormona duyarlı lipazın (HSL) aktivasyonu siklik adenozin monofosfat (cAMP) bağımlı protein kinaz A (PKA) uyarılmasını aktive eder ve lipolize neden olur. Bu nedenle, bazal ve uyarılmış lipoliz ölçümü, bu prosese dahil olan proteinlerin aktivitesini analiz etmek için önemlidir. Ayrıca, lipoliz moleküler düzenleme çözülüyor obezite 10 karşı yeni tedavi stratejilerinin geliştirilmesi faydalı olabilir. Lipoliz ve yağ asidi oksidasyonunu uyaran moleküller, depolarda depolanan yağları azaltmak için potansiyel aday olduğundan, tekrarlanabilirlik için sağlam bir test kullanmak önemlidir. Daha önce yayınlanmış veriler, beyaz yağ dokusunda ifade edilen TRPV1 proteininin CAP ile güçlendirilmiş bazalVe kasık yağ pedlerinde FSK (adenillyla siklaz aktivatörü) uyarılmış lipoliz 11 . Önceki araştırmalar ayrıca, TRPV1&#39;in CAP tarafından uzun süreli aktive edilmesinin PKA 12&#39;yi aktive ettiğini ileri sürmektedir. PKA&#39;nın aktivasyonu, lipozizi uyardığından , 13 , 14 , ilgili diyetlerin beslenmesinden 32 hafta sonra NCD veya HFD (± CAP) ile beslenen farelerden izole edilmiş kasık yağ pedlerinde bazal ve PKA&#39;ya bağlı uyarılmış lipolizin ölçülmesiyle TRPV1&#39;in rolü doğrulanacaktır Lipoliz aktivasyonu. Bu makale, bazal ve uyarılan lipolizin belirlenmesinde etkili bir yöntem anlatmaktadır. Gliserolün radyoaktif izotoplarını kullanan diğer yöntemler ve ölçümler 15 , 16 için sıkıcı yüksek performanslı sıvı kromatografisi veya gaz kromatografisi / kütle spektrometresi mevcut olsa da, bu yöntem daha doğrudan, basit ve uygun maliyetli bir yöntem sunmaktadırYağ dokusundaki lipolizi belirleme tekniği.

<table border="0" cellpadding="0" cellspacing="0" width="694">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Capsaicin</td>
			<td style="width:171px;">Sigma, USA</td>
			<td style="width:119px;">M2028</td>
			<td style="width:189px;">TRPV1 agonist</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Forskolin</td>
			<td style="width:171px;">Sigma, USA</td>
			<td style="width:119px;">F6886</td>
			<td>Adenylyl cyclase activator</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">DMEM</td>
			<td style="width:171px;">GE healthcare and life sciences, UT, USA</td>
			<td style="width:119px;">SH30081.01</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">High fat diet</td>
			<td style="width:171px;">Research diets, New Brunswick, USA</td>
			<td style="width:119px;">D12492</td>
			<td>Abbreviated as HFD</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Tris</td>
			<td style="width:171px;">Amresco, USA</td>
			<td style="width:119px;">O497</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Sodium chloride</td>
			<td style="width:171px;">Thermofisher Scientific</td>
			<td style="width:119px;">BP358-212</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Sodium deoxycholate</td>
			<td style="width:171px;">Sigma, USA</td>
			<td style="width:119px;">D6750</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Dithiothreitol</td>
			<td style="width:171px;">Sigma, USA</td>
			<td style="width:119px;">D9163</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Sodium orthovanadate</td>
			<td style="width:171px;">Sigma, USA</td>
			<td style="width:119px;">S6508</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Protease inhibitor cocktail</td>
			<td style="width:171px;">Sigma, USA</td>
			<td style="width:119px;">P8340</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Free Glycerol reagent</td>
			<td style="width:171px;">Sigma USA</td>
			<td style="width:119px;">F6428</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">DMSO</td>
			<td style="width:171px;">Sigma, USA</td>
			<td style="width:119px;">D8779</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Triacsin C</td>
			<td style="width:171px;">Sigma, USA</td>
			<td style="width:119px;">T4540</td>
			<td>Acyl CoA transferase inhibitor</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Bovine serum albumin</td>
			<td style="width:171px;">Sigma, USA</td>
			<td style="width:119px;">A7030</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Chloroform</td>
			<td style="width:171px;">Sigma Aldrich</td>
			<td style="width:119px;">31998-8</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Methanol</td>
			<td style="width:171px;">Thermofisher Scientific, USA</td>
			<td style="width:119px;">A412-1</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Sodium hydroxide</td>
			<td style="width:171px;">Amresco, USA</td>
			<td style="width:119px;">O583</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Sodium dodecyl sulfate</td>
			<td style="width:171px;">Sigma, USA</td>
			<td style="width:119px;">L3371</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Bicinchoninic acid reagent</td>
			<td style="width:171px;">Sigma, USA</td>
			<td style="width:119px;">BCA1-1KT</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">UV-VIS Spectrophotometer</td>
			<td style="width:171px;">Pharmacia Biotech, NJ, USA</td>
			<td style="width:119px;">Ultrospec 2000</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Normal chow diet</td>
			<td style="width:171px;">Labdiet.com</td>
			<td style="width:119px;">500I</td>
			<td>abreviated as NCD</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">C57BL/6 mice</td>
			<td style="width:171px;">Jackson Laboratory, CT, USA</td>
			<td style="width:119px;">Stock number000664</td>
			<td>wild type mice</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Parafilm</td>
			<td style="width:171px;">Heathrow Scientific, USA</td>
			<td style="width:119px;">HS 234526A</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Glycerol standard</td>
			<td style="width:171px;">Sigma, USA</td>
			<td style="width:119px;">G7793</td>
			<td></td>
		</tr>
	</tbody>
</table>

measurement of basal and forskolin-stimulated lipolysis in inguinal adipose fat pads

Lipoliz, yağ dokularında trigliserit olarak depolanan lipitin, gliserol ve yağ asitlerine hidrolize edilmesiyle ilgili bir işlemdir. Bu makale, normal yemeklik diyet (NCD), yüksek yağlı diyet (HFD) ya da 0.01&#39;i içeren yüksek yağlı bir diyet ile beslenen vahşi tipli farelerden izole edilen kasıktaki yağ yastıklarında bazal ve forskolin (FSK) uyarılmış lipolizin ölçülmesine yönelik yöntemi açıklamaktadır % Kapsaisin (CAP; geçici reseptör potansiyeli vaniloid altfamilı 1 (TRPV1) agonisti) 32 hafta boyunca. Ex vivo lipoliz gerçekleştirmek için burada tarif edilen yöntem Schweiger ve ark. 1 UV-Görünür (UV / VIS) spektrofotometri ile gliserol düzeylerini ölçmek için ayrıntılı bir protokol sunmaktayız. Burada açıklanan yöntem, tutarlı sonuçlar elde etmek için kasık yağ pedlerinin lipoliz ölçümleri için başarıyla izole edilmesi için kullanılabilir. Kasık yağ pedleri için tanımlanan protokol, diğer dokulardaki lipolizin ölçülmesi için kolaylıkla genişletilebilir.

CAP&#39;nin bazal ve uyarılmış lipoliz üzerindeki etkisini değerlendirmek için, bu araştırma, NCD veya HFD (± CAP) -fed vahşi tipli farelerden izole edilmiş inguinal yağlı yastıklardaki lipolizi ölçtü. Kasık yağ pedleri için bazal ve FSK ile uyarılan lipoliz için temsili sonuçlar Tablo I&#39;de verilmektedir. Asil coA sentetazı inhibe eden ve TGL&#39;nin yenilenmesini önleyen Triacsin C&#39;nin varlığında bazal ve FSK ile uyarılmış gliserol...

Watch this Scientific Journal Video about Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads at JoVE.com

Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads

Bu protokol, normal yem diyetinden (NCD) veya yüksek yağlı diyetten (HFD) ± kapsaisin ile beslenen vahşi tipli farelerde elde edilen kasık yağ tabletlerinde bazal ve forkolinin uyardığı lipoliz belirleme metodunu açıklamaktadır. Lipoliz için bir indeks olarak, inguinal yağlı yağ pedlerinden gliserol salınımı ölçülmüştür.

İnguinal Yağ Yastıklarında Basal ve Forskolin ile uyarılmış Lipoliz Ölçümleri

Lipolysis is a process by which the lipid stored as triglycerides in adipose tissues are hydrolyzed into glycerol and fatty acids. This article describes ...

Research

JoVE Journal

Biochemistry

Preparation of Keratin Hydrolysate from Chicken Feathers and Its Application in Cosmetics

Keratin hidrolizat tavuk tüyleri ve kozmetik kendi uygulamasında hazırlanması

Keratin hydrolysates (KHs) are established standard components in hair cosmetics. Understanding the moisturizing effects of KH is advantageous for ...

Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana

Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana

Yalıtım ve solunum ölçümleri mitokondri Arabidopsis thaliana üzerinden

Mitochondria are essential organelles involved in numerous metabolic pathways in plants, most notably the production of adenosine triphosphate (ATP) from ...

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

Süperoksit/hidrojen peroksit ve NADH üretim mitokondriyal Dehydrogenases Flavin içeren tarafından eş zamanlı ölçüm

It has been reported that mitochondria can contain up to 12 enzymatic sources of reactive oxygen species (ROS). A majority of these sites include ...

Mouse Adipose Tissue Collection and Processing for RNA Analysis

Fare yağ dokusu toplama ve işleme RNA analizi için

Compared to other tissues, white adipose tissue has a considerably less RNA and protein content for downstream applications such as real-time PCR and ...

Measurement of Mitochondrial Oxygen Consumption in Permeabilized Fibers of Drosophila Using Minimal Amounts of Tissue

Doku çok az miktarda kullanarak Drosophila Permeabilized liflerinin mitokondrial oksijen harcama ölçme noktalar

The fruit fly, Drosophila melanogaster, represents an emerging model for the study of metabolism. Indeed, drosophila have structures homologous to human ...

In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria

In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria

Situ Ölçüm ve korelasyon hücre yoğunluğu ve kollarındaki bakterilerin ışık emisyon

There is a considerable number of bacterial species capable of emitting light. All of them share the same gene cluster, namely the lux operon. Despite ...

Cortisol Measurement in Koala (Phascolarctos cinereus) Fur

Cortisol Measurement in Koala Phascolarctos cinereus Fur

Koalak'da Kortizol Ölçümü (Phascolarctos cinereus) Kürk

Optimal methods of hormone extraction used to measure stress in animals across sample types are not always the same. Australia&#39;s iconic marsupial ...

Direct Measurement of KDM1A Target Engagement Using Chemoprobe-based Immunoassays

Chemoprobe tabanlı bağışıklık sistemini kullanarak KDM1A hedef etkileşimi doğrudan ölçümü

The assessment of the target engagement, defined as the interaction of a drug with the protein it was designed for, is a basic requirement for the ...

Measurement of Fatty Acid &#946;-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes

Measurement of Fatty Acid ÃƒÅ½Ã‚Â²-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes

Yeni İzole Edilmiş Fare Hepatositlerinin Süspansiyonunda Yağ Asidi β-Oksidasyonunun Ölçülmesi

Fatty acid &#946;-oxidation is a key metabolic pathway to meet the energy demands of the liver and provide substrates and cofactors for additional ...

Measurement of Protein Import Capacity of Skeletal Muscle Mitochondria

İskelet Kas Mitokondrilerinin Protein İthalat Kapasitesinin Ölçümü

Mitochondria are key metabolic and regulatory organelles that determine the energy supply as well as the overall health of the cell. In skeletal muscle, ...