Name: a graphical user interface for software-assisted tracking of protein concentration in dynamic cellular protrusions
Uploaded: 2017-07-11T14:00:00
Description: Watch this Scientific Journal Video about A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions at JoVE.com

लेखकों ने डीएफजी (एक्ससी -1003 से एमजी) के लिए धन को स्वीकार किया है

1. सेल संस्कृति <ol><li> डल्बेइको के संशोधित ईगल मध्यम (डीएमईएम) में संस्कृति हेला या सीओएस कोशिकाओं में 4.5 ग्राम एल एल ग्लूकोस, एल एलानिन-एल-ग्लूटामाइन डायपेप्टाइड, प्यूरवेट, 10% भ्रूण गोजातीय सीरम और पेनिसिल?...

<ol>
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B., Johnson, M. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+quantitative+study+of+growth+cone+filopodial+extension.">A quantitative study of growth cone filopodial extension.</a> J Neurosci Res. 13 (1-2), 149-162 (1985).</li><li>Mogilner, A., Rubinstein, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+physics+of+filopodial+protrusion.">The physics of filopodial protrusion.</a> Biophys J. 89 (2), 782-795 (2005).</li><li>Shaner, N. C., Steinbach, P. A., Tsien, R. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+guide+to+choosing+fluorescent+proteins.">A guide to choosing fluorescent proteins.</a> Nat Methods. 2 (12), 905-909 (2005).</li><li>Abramoff, M. D., Magalhaes, P. J., Ram, S. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Image+Processing+with+ImageJ.">Image Processing with ImageJ.</a> Biophotonics International. 11, 36-42 (2004).</li><li>Courtney, J., Woods, E., Scholz, D., Hall, W. W., Gautier, V. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=MATtrack:+A+MATLAB-Based+Quantitative+Image+Analysis+Platform+for+Investigating+Real-Time+Photo-Converted+Fluorescent+Signals+in+Live+Cells.">MATtrack: A MATLAB-Based Quantitative Image Analysis Platform for Investigating Real-Time Photo-Converted Fluorescent Signals in Live Cells.</a> PLoS One. 10 (10), e0140209 (2015).</li><li>Schell, M. J., Erneux, C., Irvine, R. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inositol+1,4,5-trisphosphate+3-kinase+A+associates+with+F-actin+and+dendritic+spines+via+its+N+terminus.">Inositol 1,4,5-trisphosphate 3-kinase A associates with F-actin and dendritic spines via its N terminus.</a> J Biol Chem. 276 (40), 37537-37546 (2001).</li><li>Korobova, F., Svitkina, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+architecture+of+synaptic+actin+cytoskeleton+in+hippocampal+neurons+reveals+a+mechanism+of+dendritic+spine+morphogenesis.">Molecular architecture of synaptic actin cytoskeleton in hippocampal neurons reveals a mechanism of dendritic spine morphogenesis.</a> Mol Biol Cell. 21 (1), 165-176 (2010).</li><li>Cheadle, L., Biederer, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+novel+synaptogenic+protein+Farp1+links+postsynaptic+cytoskeletal+dynamics+and+transsynaptic+organization.">The novel synaptogenic protein Farp1 links postsynaptic cytoskeletal dynamics and transsynaptic organization.</a> J Cell Biol. 199 (6), 985-1001 (2012).</li><li>Tarnok, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+new+tool+for+the+quantitative+analysis+of+dendritic+filopodial+motility.">A new tool for the quantitative analysis of dendritic filopodial motility.</a> Cytometry A. 87 (1), 89-96 (2015).</li><li>Hendricusdottir, R., Bergmann, J. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=F-dynamics:+automated+quantification+of+dendrite+filopodia+dynamics+in+living+neurons.">F-dynamics: automated quantification of dendrite filopodia dynamics in living neurons.</a> J Neurosci Methods. 236, 148-156 (2014).</li><li>Fanti, Z., Martinez-Perez, M. E., De-Miguel, F. 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यहां हम फिलाओपोडियल ग्रोथ डायनामिक्स पर नज़र रखने और इन गतिशील संरचनाओं में सापेक्ष प्रोटीन सांद्रता के विश्लेषण को उत्तल-हल एल्गोरिदम के माध्यम से विस्तृत प्रोटोकॉल पेश करते हैं। सॉफ़्टवेयर का उप?...

<table border="0" cellpadding="0" cellspacing="0" width="816">
	<tbody>
		<tr height="41">
			<td height="41" style="height:41px;width:204px;">DMEM</td>
			<td style="width:204px;">Life Technologies</td>
			<td style="width:204px;">31966-021</td>
			<td style="width:204px;"></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:204px;">10% Fetal bovine serum</td>
			<td style="width:204px;">Biochrom AG</td>
			<td style="width:204px;">L11-044</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:204px;">Lipofectamine 2000</td>
			<td style="width:204px;">Life Technologies</td>
			<td style="width:204px;">11668-027</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:204px;">1% penicillin/streptomycin</td>
			<td style="width:204px;">Biochrom AG</td>
			<td style="width:204px;">12212</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:204px;">Neurobasal Medium</td>
			<td style="width:204px;">Life Technologies</td>
			<td style="width:204px;">21103-049</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:204px;">B27</td>
			<td style="width:204px;">Life Technologies</td>
			<td style="width:204px;">17504-044</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:204px;">HEPES (1M stock solution)</td>
			<td style="width:204px;">Life Technologies</td>
			<td style="width:204px;">15630</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:204px;">Citrine-N1</td>
			<td style="width:204px;">Addgene</td>
			<td style="width:204px;">54593</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;">Labtech</td>
			<td>Thermo</td>
			<td>155411</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:204px;">Glutamax-I</td>
			<td>Thermo</td>
			<td>35050-061</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:204px;">Hela</td>
			<td>Leibniz Institute DSMZ</td>
			<td>ACC-57</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:204px;">COS 7</td>
			<td>Leibniz Institute DSMZ</td>
			<td>ACC-60</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:204px;">3T3 cells</td>
			<td>Leibniz Institute DSMZ</td>
			<td>ACC-59</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:204px;">Microscope</td>
			<td>Nicon Eclipse</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:204px;">Camera</td>
			<td>Andor</td>
			<td>DU888 Ultra</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:204px;">Confocal Unit</td>
			<td>Yokagawa</td>
			<td>CSU-X1</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:204px;">Pyruvate</td>
			<td>Gibco</td>
			<td>31966-021</td>
			<td></td>
		</tr>
	</tbody>
</table>

a graphical user interface for software-assisted tracking of protein concentration in dynamic cellular protrusions

फिलिपोडिया गतिशील, माइग्रेशन और सेल-सेल संचार से जुड़ी उंगली की तरह सेलुलर प्रोट्रुशन हैं। फैलोपोडियल डायनिंग, बढ़ाव और बाद के स्थिरीकरण या पीछे हटने के अंतर्निहित जटिल सिग्नलिंग तंत्रों को बेहतर ढंग से समझने के लिए, इन गतिशील संरचनाओं में स्थैर-अस्थायी प्रोटीन गतिविधि निर्धारित करना महत्वपूर्ण है। फिलीपीडिया में प्रोटीन समारोह का विश्लेषण करने के लिए, हमने हाल ही में एक अर्ध-स्वचालित ट्रैकिंग एल्गोरिदम विकसित किया है जो कि फेयोडोडायडियल आकृति-परिवर्तनों के लिए अनुकूल है, इस प्रकार पूरे फैलोडोडाियल लंबाई के साथ फलाव गतिशीलता और रिश्तेदार प्रोटीन एकाग्रता के समानांतर विश्लेषण की अनुमति देता है। यहां, हम अनुकूलित सेल हैंडलिंग, छवि अधिग्रहण और सॉफ्टवेयर विश्लेषण के लिए एक विस्तृत चरण-दर-चरण प्रोटोकॉल प्रस्तुत करते हैं। हम आगे छवि विश्लेषण और डेटा प्रतिनिधित्व के दौरान वैकल्पिक सुविधाओं के उपयोग के लिए निर्देश प्रदान करते हैं, साथ ही रास्ते में सभी महत्वपूर्ण कदमों के लिए समस्या निवारण दिशानिर्देश प्रदान करते हैं। अंत में, हम डी की तुलना भी करते हैंफ़िलापोडायिया मात्रा का ठहराव के लिए उपलब्ध अन्य कार्यक्रमों के साथ लिखित छवि विश्लेषण सॉफ्टवेयर साथ में, प्रस्तुत प्रोटोकॉल छवि विश्लेषण सॉफ्टवेयर का प्रयोग करके फिलीपीडियल प्रोट्रूशियन्स में प्रोटीन गतिशीलता के सटीक विश्लेषण के लिए एक रूपरेखा प्रदान करता है।

फिलामेंटस एक्टिन (एफ-ट्रैक्टिन 18 , लाल) और एक साइटोस्लिक संदर्भ (हरा) के लिए एक मार्कर के साथ ट्रांसफ़ेक्ट सीओएस कोशिकाओं का उपयोग करते हुए, हमने एक्टिन-अमीर फिलिपोडियल प्रोट्रूशि?...

Watch this Scientific Journal Video about A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions at JoVE.com

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions

हम गतिशील सेलुलर प्रोट्रूशियन्स की लंबाई के साथ सापेक्ष प्रोटीन एकाग्रता की अर्ध-स्वचालित ट्रैकिंग के लिए एक सॉफ्टवेयर समाधान प्रस्तुत करते हैं।

डायनामिक सेलुलर प्रोट्र्रेशन में प्रोटीन एकाग्रता के सॉफ़्टवेयर-सहायता प्राप्त ट्रैकिंग के लिए एक ग्राफ़िकल यूजर इंटरफेस

Filopodia are dynamic, finger-like cellular protrusions associated with migration and cell-cell communication. In order to better understand the complex ...

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लाइट ठीक चिकित्सकीय चिपकने वाला राल के बढ़ते जीर्ण प्रयोगों में इलेक्ट्रोड या microdialysis जांच के लिए आवेदन

In chronic recording experiments, self-curing dental acrylic resins have been used as a mounting base of electrodes or microdialysis-probes. Since these ...

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Flying insects use visual cues to stabilize their heading in a wind stream. Many animals additionally track odors carried in the wind. As such, visual ...

Microvolume Protein Concentration Determination using the NanoDrop 2000c Spectrophotometer

Microvolume प्रोटीन एकाग्रता निर्धारण का उपयोग NanoDrop 2000C स्पेक्ट्रोफोटोमीटर

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Split-Ubiquitin Based Membrane Yeast Two-Hybrid MYTH System: A Powerful Tool For Identifying Protein-Protein Interactions

भाजित Ubiquitin आधार झिल्ली खमीर (मिथक) दो - हाइब्रिड सिस्टम: प्रोटीन, प्रोटीन बातचीत की पहचान करने के लिए एक शक्तिशाली उपकरण

The fundamental biological and clinical importance of integral membrane proteins prompted the development of a yeast-based system for the high-throughput ...

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis 

कैनेटीक्स और संरचना समारोह विश्लेषण में आत्मीयता निर्धारण के लिए सही प्रोटीन एकाग्रता का महत्व

In this study, we explore the interaction between the bovine cysteine protease inhibitor cystatin B and a catalytically inactive form of papain (Fig. 1), ...

A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients

लोकतंत्र एकाग्रता gradients में बैक्टीरियल chemotaxis बढ़ाता के लिए एक microfluidic युक्ति

Chemotaxis allows bacteria to approach sources of attractant chemicals or to avoid sources of repellent chemicals. Bacteria constantly monitor the ...

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

अस्थिर मल्टी प्रोटीन परिसर के अलगाव द्वारा Vivo में नियंत्रित पार से जोड़ने और इम्यूनो - चुंबकीय आत्मीयता क्रोमैटोग्राफी सेलुलर

The dynamic nature of cellular machineries is frequently built on transient and/or weak protein associations. These low affinity interactions preclude ...

Analysis of the Development of a Morphological Phenotype as a Function of Protein Concentration in Budding Yeast

नवोदित खमीर में प्रोटीन कांसंट्रेशन की एक फंक्शन के रूप एक morphological Phenotype के विकास विश्लेषण

Gene deletion and protein overexpression are common methods for studying functions of proteins. In this article, we describe a protocol for analysis of ...

Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo

प्रारंभिक चिकी भ्रूण में Morphogenetic ऊतक विरुपणों ट्रैकिंग

Embryonic epithelia undergo complex deformations (e.g. bending, twisting, folding, and stretching) to form the primitive organs of the early embryo. ...

Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos

Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos

Illustrating के गतिशील kinetochore प्रोटीन और रहने में क्रोमोजोम प्रस्ताव व्यवहार के द्वारा mitotic चेकप्वाइंट अध्ययन<em&gt; ड्रोसोफिला</em&gt; Syncytial भ्रूण

The spindle assembly checkpoint (SAC) mechanism is an active signal, which monitors the interaction between chromosome kinetochores and spindle ...