Name: preparation of herbal medicine: er-xian decoction and er-xian-containing serum for in vivo and in vitro experiments
Uploaded: 2017-05-31T15:00:00
Description: Watch this Scientific Journal Video about Preparation of Herbal Medicine: Er-Xian Decoction and Er-Xian-containing Serum for In Vivo and In Vitro Experiments at JoVE.com

Dit werk werd ondersteund door de National Natural Science Foundation of China (81573992). We bedanken Emily K. Lo en Kathleen DiNapoli voor hun taalbewerkingshulp.

Het protocol volgt de richtlijnen voor dierenwelzijn van de Shanghai University of Traditional Chinese Medicine en is goedgekeurd door het dierproefbedrijf Shanghai Animal Ethics Committee. 1. Protocol I: Bereiding van EXD <ol><li> Berekening <ol><li> Beheer de EXD aan een behandelingsgroep van 10 zes maanden oude vrouwelijke Impressive Control Region (ICR) muizen (0,02 kg / muis), 5 dagen per week gedurende 12 weken. OPMERKING: er zijn in totaal 1.702 EXD&#39;s nod...

<ol>
	<li>Zhang, L. G., Ouyang, X. W., Wu, T. T., Ni, L. J., Shi, W. Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantitative+evaluation+of+in+vitro+effects+and+interactions+of+active+fractions+in+a+Chinese+medicinal+formula+(Yaotongning+Capsule)+on+rat+chondrocytes.">Quantitative evaluation of in vitro effects and interactions of active fractions in a Chinese medicinal formula (Yaotongning Capsule) on rat chondrocytes.</a> J Ethnopharmacol. 155 (3), 1424-1432 (2014).</li><li>Ogawa, Y., Fujii, Y., Sugiyama, R., Konishi, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+role+of+the+seven+crude+drug+components+in+the+sleep-promoting+effect+of+Yokukansan.">The role of the seven crude drug components in the sleep-promoting effect of Yokukansan.</a> J Ethnopharmacol. 177, 19-27 (2016).</li><li>Kim, J. H., Doh, E. J., Lee, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evaluation+of+Medicinal+Categorization+of+Atractylodes+japonica+Koidz.+by+Using+Internal+Transcribed+Spacer+Sequencing+Analysis+and+HPLC+Fingerprinting+Combined+with+Statistical+Tools.">Evaluation of Medicinal Categorization of Atractylodes japonica Koidz. by Using Internal Transcribed Spacer Sequencing Analysis and HPLC Fingerprinting Combined with Statistical Tools.</a> Evid Based Complement Alternat Med. 2016, 2926819 (2016).</li><li>Sheridan, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+potential+of+metabolic+fingerprinting+as+a+tool+for+the+modernisation+of+TCM+preparations.">The potential of metabolic fingerprinting as a tool for the modernisation of TCM preparations.</a> J Ethnopharmacol. 140 (3), 482-491 (2012).</li><li>Planas, G. M., Kucacute, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Contraceptive+Properties+of+Stevia+rebaudiana.">Contraceptive Properties of Stevia rebaudiana.</a> Science. 162 (3857), 1007 (1968).</li><li>Tashino, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term="Serum+pharmacology"+and+"serum+pharmaceutical+chemistry":+from+pharmacology+of+Chinese+traditional+medicines+to+start+a+new+measurement+of+drug+concentration+in+blood.">"Serum pharmacology" and "serum pharmaceutical chemistry": from pharmacology of Chinese traditional medicines to start a new measurement of drug concentration in blood.</a> Ther Drug Monit Res. 5, 54-64 (1988).</li><li>Cao, Y., Liu, F., Huang, Z., Zhang, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Protective+effects+of+Guanxin+Shutong+capsule+drug-containing+serum+on+tumor+necrosis+factor-alpha-induced+endothelial+dysfunction+through+nicotinamide+adenine+dinucleotide+phosphate+oxidase+and+the+nitric+oxide+pathway.">Protective effects of Guanxin Shutong capsule drug-containing serum on tumor necrosis factor-alpha-induced endothelial dysfunction through nicotinamide adenine dinucleotide phosphate oxidase and the nitric oxide pathway.</a> Exp Ther Med. 8 (3), 998-1004 (2014).</li><li>Fu, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ex+Vivo+Stromal+Cell-Derived+Factor+1-Mediated+Differentiation+of+Mouse+Bone+Marrow+Mesenchymal+Stem+Cells+into+Hepatocytes+Is+Enhanced+by+Chinese+Medicine+Yiguanjian+Drug-Containing+Serum.">Ex Vivo Stromal Cell-Derived Factor 1-Mediated Differentiation of Mouse Bone Marrow Mesenchymal Stem Cells into Hepatocytes Is Enhanced by Chinese Medicine Yiguanjian Drug-Containing Serum.</a> Evid Based Complement Alternat Med. 2016, 7380439 (2016).</li><li>Chen, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Application+of+serum+pharmacology+in+evaluating+the+antitumor+effect+of+Fuzheng+Yiliu+Decoction+from+Chinese+Medicine.">Application of serum pharmacology in evaluating the antitumor effect of Fuzheng Yiliu Decoction from Chinese Medicine.</a> Chin J Integr Med. 20 (6), 450-455 (2014).</li><li>Sze, S. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+mechanism:+Erxian+Decoction,+a+Chinese+medicine+formula,+for+relieving+menopausal+syndrome.">A novel mechanism: Erxian Decoction, a Chinese medicine formula, for relieving menopausal syndrome.</a> J Ethnopharmacol. 123 (1), 27-33 (2009).</li><li>Sze, S. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+Erxian+decoction,+a+Chinese+medicinal+formulation,+on+serum+lipid+profile+in+a+rat+model+of+menopause.">Effects of Erxian decoction, a Chinese medicinal formulation, on serum lipid profile in a rat model of menopause.</a> Chin Med. 6, 40 (2011).</li><li>Zhong, L. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+randomized,+double-blind,+controlled+trial+of+a+Chinese+herbal+formula+(Er-Xian+decoction)+for+menopausal+symptoms+in+Hong+Kong+perimenopausal+women.">A randomized, double-blind, controlled trial of a Chinese herbal formula (Er-Xian decoction) for menopausal symptoms in Hong Kong perimenopausal women.</a> Menopause. 20 (7), 767-776 (2013).</li><li>Wang, S. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Steroidogenic+effect+of+Erxian+decoction+for+relieving+menopause+via+the+p-Akt/PKB+pathway+in+vitro+and+in+vivo.">Steroidogenic effect of Erxian decoction for relieving menopause via the p-Akt/PKB pathway in vitro and in vivo.</a> J Ethnopharmacol. , (2016).</li><li>Lin, L., Wu, S., Tang, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=[Clinical+observation+and+experimental+study+of+the+treatment+of+aplastic+anemia+by+warming+and+tonifying+the+spleen+and+kidney].">[Clinical observation and experimental study of the treatment of aplastic anemia by warming and tonifying the spleen and kidney].</a> Zhong Xi Yi Jie He Za Zhi. 10 (5), 272-274 (1990).</li><li>Nian, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Antiosteoporotic+activity+of+Er-Xian+Decoction,+a+traditional+Chinese+herbal+formula,+in+ovariectomized+rats.">Antiosteoporotic activity of Er-Xian Decoction, a traditional Chinese herbal formula, in ovariectomized rats.</a> J Ethnopharmacol. 108 (1), 96-102 (2006).</li><li>Qin, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Antiosteoporotic+chemical+constituents+from+Er-Xian+Decoction,+a+traditional+Chinese+herbal+formula.">Antiosteoporotic chemical constituents from Er-Xian Decoction, a traditional Chinese herbal formula.</a> J Ethnopharmacol. 118 (2), 271-279 (2008).</li><li>Xue, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+HNMR-based+metabonomics+study+of+postmenopausal+osteoporosis+and+intervention+effects+of+Er-Xian+Decoction+in+ovariectomized+rats.">A HNMR-based metabonomics study of postmenopausal osteoporosis and intervention effects of Er-Xian Decoction in ovariectomized rats.</a> Int J Mol Sci. 12 (11), 7635-7651 (2011).</li><li>Lu, X. N., Xu, X. R., Lin, L. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=[Clinical+observation+of+bushen+er'xian+decoction+in+treating+premature+ovarian+failure].">[Clinical observation of bushen er'xian decoction in treating premature ovarian failure].</a> Zhongguo Zhong Xi Yi Jie He Za Zhi. 28 (7), 594-596 (2008).</li><li>Yu, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Anti-angiogenic+activity+of+Erxian+Decoction,+a+traditional+Chinese+herbal+formula,+in+zebrafish.">Anti-angiogenic activity of Erxian Decoction, a traditional Chinese herbal formula, in zebrafish.</a> Biol Pharm Bull. 35 (12), 2119-2127 (2012).</li><li>Chu, E. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+in+vitro+and+in+vivo+investigation+of+the+antimetastatic+effects+of+a+Chinese+medicinal+decoction,+erxian+decoction,+on+human+ovarian+cancer+models.">An in vitro and in vivo investigation of the antimetastatic effects of a Chinese medicinal decoction, erxian decoction, on human ovarian cancer models.</a> Integr Cancer Ther. 12 (4), 336-346 (2013).</li><li>Zhu, Z., Li, L., Jin, X., Fang, J., Zhang, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Er-Xian+Decoction,+a+traditional+Chinese+herbal+formula,+intervening+early+in+hypothalamic-pituitary+axis+of+male+rats+with+delayed+puberty.">Er-Xian Decoction, a traditional Chinese herbal formula, intervening early in hypothalamic-pituitary axis of male rats with delayed puberty.</a> Pharmacogn Mag. 10 (40), 517-521 (2014).</li><li>Miao, M. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Experimental animals and technology. 1997, 145 (1997).</li><li>Soleimani, M., Nadri, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+protocol+for+isolation+and+culture+of+mesenchymal+stem+cells+from+mouse+bone+marrow.">A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow.</a> Nat Protoc. 4 (1), 102-106 (2009).</li><li>Lu, S., Huang, J., Wang, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Er-Xian+Decoction+Stimulates+Osteoblastic+Differentiation+of+Bone+Mesenchymal+Stem+Cells+in+Ovariectomized+Mice+and+Its+Gene+Profile+Analysis.">Er-Xian Decoction Stimulates Osteoblastic Differentiation of Bone Mesenchymal Stem Cells in Ovariectomized Mice and Its Gene Profile Analysis.</a> Stem Cells International. 2016, 4079210 (2016).</li><li>Turner, P. V., Brabb, T., Pekow, C., Vasbinder, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Administration+of+substances+to+laboratory+animals:+routes+of+administration+and+factors+to+consider.">Administration of substances to laboratory animals: routes of administration and factors to consider.</a> J Am Assoc Lab Anim Sci. 50 (5), 600-613 (2011).</li><li>Wu, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Simultaneous+determination+of+seven+flavonoids+in+dog+plasma+by+ultra-performance+liquid+chromatography-tandem+mass+spectrometry+and+its+application+to+a+bioequivalence+study+of+bioactive+components+in+Herba+Epimedii+and+Er-Xian+Decoction.">Simultaneous determination of seven flavonoids in dog plasma by ultra-performance liquid chromatography-tandem mass spectrometry and its application to a bioequivalence study of bioactive components in Herba Epimedii and Er-Xian Decoction.</a> J Pharm Biomed Anal. 54 (1), 186-191 (2011).</li><li>Hu, Y. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Identification+of+the+major+chemical+constituents+and+their+metabolites+in+rat+plasma+and+various+organs+after+oral+administration+of+effective+Erxian+Decoction+(EXD)+fraction+by+liquid+chromatography-mass+spectrometry.">Identification of the major chemical constituents and their metabolites in rat plasma and various organs after oral administration of effective Erxian Decoction (EXD) fraction by liquid chromatography-mass spectrometry.</a> Biomed Chromatogr. 24 (5), 479-489 (2010).</li><li>Xue, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+and+interaction+of+icariin,+curculigoside,+and+berberine+in+er-xian+decoction,+a+traditional+chinese+medicinal+formula,+on+osteoclastic+bone+resorption.">Effects and interaction of icariin, curculigoside, and berberine in er-xian decoction, a traditional chinese medicinal formula, on osteoclastic bone resorption.</a> Evid Based Complement Alternat Med. , (2012).</li><li>Nair, A. B., Jacob, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+simple+practice+guide+for+dose+conversion+between+animals+and+human.">A simple practice guide for dose conversion between animals and human.</a> J Basic Clin Pharm. 7 (2), 27-31 (2016).</li><li>Xu, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Protective+effect+of+the+traditional+Chinese+medicine+xuesaitong+on+intestinal+ischemia-reperfusion+injury+in+rats.">Protective effect of the traditional Chinese medicine xuesaitong on intestinal ischemia-reperfusion injury in rats.</a> Int J Clin Exp Med. 8 (2), 1768-1779 (2015).</li><li>Li, Z., Wang, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=On+the+methods+for+Chinese+herbs+serum+pharmcology.">On the methods for Chinese herbs serum pharmcology.</a> Zhong Guo Zhong Yi Yao Xing Xi Za Zhi. 9 (2), 5-6 (2002).</li><li>Jiang, Y. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+chinese+herbal+drug-containing+serum+for+activating-blood+and+dispelling-toxin+on+ox-LDL-induced+inflammatory+factors'+expression+in+endothelial+cells.">Effect of chinese herbal drug-containing serum for activating-blood and dispelling-toxin on ox-LDL-induced inflammatory factors' expression in endothelial cells.</a> Chin J Integr Med. 18 (1), 30-33 (2012).</li><li>Guo, C. Y., Ma, X. J., Liu, Q., Yin, H. J., Shi, D. Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=[Effect+of+Chinese+herbal+drug-containing+serum+for+activating+blood,+activating+blood+and+dispelling+toxin+on+TNF-alpha-induced+adherence+between+endothelial+cells+and+neutrophils+and+the+expression+of+MAPK+pathway].">[Effect of Chinese herbal drug-containing serum for activating blood, activating blood and dispelling toxin on TNF-alpha-induced adherence between endothelial cells and neutrophils and the expression of MAPK pathway].</a> Zhongguo Zhong Xi Yi Jie He Za Zhi. 35 (2), 204-209 (2015).</li><li>Li, Y., Xia, J. Y., Chen, W., Deng, C. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+Ling+Qi+Juan+Gan+capsule+drug-containing+serum+on+PDGF-induced+proliferation+and+JAK/STAT+signaling+of+HSC-T6+cells].">Effects of Ling Qi Juan Gan capsule drug-containing serum on PDGF-induced proliferation and JAK/STAT signaling of HSC-T6 cells].</a> Zhonghua Gan Zang Bing Za Zhi. 21 (9), 663-667 (2013).</li><li>Li, Y. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=[Some+issues+in+methology+of+Chinese+herbs+serum+pharmcology].">[Some issues in methology of Chinese herbs serum pharmcology].</a> Zhong Yao Xin Yao Yu Lin Chuang Yao Li. 10 (5), 263 (1999).</li><li>Zhang, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=[A+review+of+Chinese+herbs+serum+pharmcology+methodological+study].">[A review of Chinese herbs serum pharmcology methodological study].</a> Nan Jing Zhong Yi Yao Da Xue Xue Bao. 18 (4), 254 (2002).</li><li>Zhang, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=[Issues+and+strategies+for+study+of+serum+pharmcology+in+oncology].">[Issues and strategies for study of serum pharmcology in oncology].</a> Zhong Yi Yan Jiu. 17 (5), 13-14 (2004).</li></ol>

In de afgelopen jaren is meer aandacht besteed aan kruidengeneesmiddelen, een soort alternatief geneesmiddel dat al duizenden jaren in de klinische omgeving in de oostelijke wereld is toegepast. Afwijkend van het &quot;bank-tot-bed&quot; -patroon van het moderne medicijn, hebben traditionele kruidengeneeskunde eerst het &#39;bed-to-bench&#39;-patroon nodig om hun mechanismen uit te leggen. Dit kan gevolgd worden door validatie uitgevoerd op de bank en verwerken voor de ontwikkeling van nieuw geoptimaliseerde drugs. Tot ...

De interesse in de studie en toepassing van traditionele kruiden geneeskunde groeit momenteel. In tegenstelling tot moderne medicijnen, waarin chemische ingrediënten zijn bepaald, bevatten kruidenformules sommige onbekende ingrediënten en worden extractieprocessen vereist om de afgifte van hun actieve verbindingen mogelijk te maken. Hoewel veel studies proberen een kleine verbinding te selecteren met een bekende structuur als representatief voor de gehele kruiden- of kruidenformule, kunnen de farmacologische efficiënties noch mechanismen als equivalent 1 , 2 worden beschouwd. Terwijl vingerafdrukken de analyse van de bestanddelen van complexe kruidenformules mogelijk maken, zijn sommige bestanddelen nog niet duidelijk geanalyseerd, wat leidt tot een uitdaging bij het combineren van alle extracten voor studie 3 . De interacties van talrijke bestanddelen / extracten bemiddelen de therapeutische effecten van kruidengeneesmiddelen. Om dit voordeel te behouden, wordt de traditionele extractvorm-decoctIon-wordt nog steeds veel gebruikt in de kliniek. Aangezien de extractieprocedure een grote invloed heeft op de therapeutische werkzaamheid, is een standaard protocol van traditionele waterafkok noodzakelijk, vooral voor in vivo studies 4 , 5 . Aan de andere kant, bij het onderzoeken van farmacologische mechanismen, is de toediening van kruiddekkingen tijdens in vitro of ex vivo studies ook een uitdaging. Het concept van een geneesmiddel bevattend serum werd voor het eerst in 1988 door Tashino voorgesteld 6 . Sindsdien heeft toenemend aantal onderzoekers het toegepast op kruidengeneeskunde 7 , 8 , 9 . Hoewel de methode van geneesmiddel bevattend serum enkele beperkingen heeft, zoals de invloed van bepaalde componenten van het serum, wordt het nog steeds beschouwd als een methode die de fysiologische omstandigheden nauwlettend nabootst. 10 , 11 , 12 , 13 te verlichten. Het is ook toegepast op de behandeling van aplastische anemie 14 , menopauzale osteoporose 15 , 16 , 17 , vroegtijdige ovariale mislukking 18 , borstkanker 19 , eierstokkanker 20 en vertraagde puberteit 21 . Hier presenteren we gedetailleerde protocollen voor de bereiding van zowel de er-xian decoction (EXD) en het geneesmiddel bevattende serum. Daarnaast beschrijven we de toepassing van EXD- en EXD-bevattende serum op muizen-menopauzale osteoporotische modellen. Een EXD bestaat uit 9 g elk van Curculigo orchioides Gaertn , Herbaa Epimedii </eM&gt; Radix Morindae Officinalis en Radix Angelicae Sinensis en 6 g elk van Cortex Phellodendri en Rhizoma Anemarrhenae per volwassen patiënt per dag. De equivalente dosis voor een muis is 0,1418 EXD / kg / dag op basis van de volgende vergelijking 22 : dB = dA * RB / RA * (WA / WB) 1/3 . DA en dB verwijzen naar de dosis per lichaamsgewicht (mg / kg) van respectievelijk de mens en muis. De dosis, in mg / kg, wordt vervangen door het aantal EXD / kg. RA en RB vertegenwoordigen respectievelijk de respectievelijke menselijke lichaamsfactor en muislichaamfactor, die evenredig zijn aan het lichaamsoppervlak (m 2 ) / lichaamsgewicht (kg)) 2/3 (zie tabel 1 ). WA en WB geven het lichaamsgewicht (kg) van de mens en muis aan.

<table border="0" cellpadding="0" cellspacing="0" width="651">
	
	<tbody>
		<tr height="165">
			<td height="165" style="height:165px;width:216px;">Curculigo orchioides Gaertn (9&#8201;g), Herbaa Epimedii (9&#8201;g), Radix Morindae Officinalis (9&#8201;g), Radix Angelicae Sinensis (9&#8201;g), Cortex Phellodendri (6&#8201;g), and Rhizoma Anemarrhenae (6&#8201;g)</td>
			<td style="width:149px;">Kang-qiao Chinese Medicine Yinpian Co. Ltd (Shanghai, CN)</td>
			<td style="width:119px;">160922</td>
			<td style="width:167px;">EXD components</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:216px;">Filter paper</td>
			<td style="width:149px;">GElifesciences</td>
			<td style="width:119px;">99-103-952</td>
			<td style="width:167px;">Filter EXD decoction before concentration</td>
		</tr>
		<tr height="60">
			<td height="60" style="height:60px;width:216px;">Imprinting Control Region (ICR) mice</td>
			<td style="width:149px;">Shanghai Laboratory Animal Center</td>
			<td style="width:119px;">SCXK 2007-0005</td>
			<td style="width:167px;">In vivo study</td>
		</tr>
		<tr height="60">
			<td height="60" style="height:60px;width:216px;">Sprague Dawley rats</td>
			<td style="width:149px;">Shanghai Laboratory Animal Center</td>
			<td style="width:119px;">SCXK 2007-0005</td>
			<td style="width:167px;">EXD-containing serum preparation</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">Syringe filter</td>
			<td style="width:149px;">Millipore</td>
			<td style="width:119px;">SLGP033RB</td>
			<td style="width:167px;">0.22 &#181;m</td>
		</tr>
		<tr height="61">
			<td height="61" style="height:61px;width:216px;">gavage needles (10 ml)</td>
			<td style="width:149px;">Shanghai BO Ge trade sales department</td>
			<td align="right" style="width:119px;">59104274</td>
			<td style="width:167px;">Adminstration of EXD</td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;">Ketamine (80 mg/kg)&#160;</td>
			<td style="width:149px;">Fujian Gutian Pharma Co. Ltd</td>
			<td style="width:119px;">H35020148</td>
			<td>Anesthesia</td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:216px;">Xylazine (10 mg/kg)</td>
			<td style="width:149px;">Sunway Pharma Co. Ltd</td>
			<td style="width:119px;">CB07591</td>
			<td>Anesthesia</td>
		</tr>
		<tr height="81">
			<td height="81" style="height:81px;width:216px;">Dulbecco&#8217;s modification of Eagle&#8217;s medium Dulbecco (DMEM) culture medium</td>
			<td style="width:149px;">Gibco</td>
			<td style="width:119px;">12800-116</td>
			<td style="width:167px;">DMEM with 2 mM L-glutamine and without ribonucleosides and ribonucleotides</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Streptomycin</td>
			<td style="width:149px;">Sigma</td>
			<td align="right" style="width:119px;">1277</td>
			<td style="width:167px;">100 &#181;g / ml</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Penicillin</td>
			<td style="width:149px;">Sigma</td>
			<td align="right" style="width:119px;">4687</td>
			<td style="width:167px;">100 &#181;g / ml</td>
		</tr>
	</tbody>
</table>

preparation of herbal medicine: er-xian decoction and er-xian-containing serum for in vivo and in vitro experiments

Traditionele kruidengeneeskunde, een alternatief geneesmiddel in de klinische omgeving, heeft de afgelopen jaren meer aandacht gekregen. Voordat u naar het lichaam overgaat, is er een extra extractieprocedure nodig om de actieve bestanddelen van ruwe kruiden te laten vrijkomen. Waterafkooksel is een klassieke extractie procedure die nog steeds in grote mate gebruikt wordt in de klinische instellingen. Hier voorstellen we een gedetailleerd protocol voor er-xian decoction (EXD) om kruidendecten toe te passen op experimentele studies. De berekening van een dierlijke dosis wordt beschreven, evenals de vier hoofdtrappen van EXD: doordrenken, waterafkooksel, filtratie en concentratie. Daarnaast wordt serum-bevattende EXD geïntroduceerd voor ratten als middel voor in vitro validatie. Hier waren ratten gedurende 3 dagen oraal toegediend met EXD. Bloedmonsters werden vervolgens verzameld, geïnactiveerd, gecentrifugeerd en gefiltreerd. Het serum, verdund met het kweekmedium, kan worden gebruikt om cellen of weefsels te behandelen in vItro. Zo werd bijvoorbeeld EXD toegepast op zowel in vivo als in vitro studies en bleek dat EXD osteogenese verbetert. Dit protocol kan gebruikt worden als referentie voor de bereiding en toepassing van kruidengeneesmiddelen.

Het effect van EXD op de botdichtheid van OVX-muizen Hematoxyline- en eosin- (H &amp; E) -vervaging van de lumbale wervelkolom toont verhoogde bottrabeculae na EXD-behandeling in vivo ( Figuur 1A , rechter paneel) vergeleken met die in de OVX-groep ( Figuur 1A , linker paneel). Figuur 1B toont de representatieve μCT beelden van de 4e lumbale in OVX muizen ( <...

Watch this Scientific Journal Video about Preparation of Herbal Medicine: Er-Xian Decoction and Er-Xian-containing Serum for In Vivo and In Vitro Experiments at JoVE.com

Preparation of Herbal Medicine: Er-Xian Decoction and Er-Xian-containing Serum for In Vivo and In Vitro Experiments

Hier presenteren we de voorbereiding van een er-xian decoctie (EXD) in vier stappen: ontdooiing, afkooksel, filtratie en concentratie- en de toediening van een bereid EXD-bevattende serum aan ratten aan te tonen. Deze methoden zijn van toepassing op de in vivo en in vitro studie van kruiddekkingen zoals traditionele Chinese geneesmiddelen.

Voorbereiding van kruidengeneeskunde: Er-Xian Decoction en Er-Xian-Serum voor<em&gt; In Vivo</em&gt; En<em&gt; In Vitro</em&gt; Experimenten

Traditional herbal medicine, an alternative medicine in the clinical setting, has received increased attention in recent years. Before delivery to the ...

Research

JoVE Journal

Medicine

Preparation of Herbal Medicine: Er-Xian Decoction and Er-Xian-containing Serum for In Vivo and In Vitro Experiments

In vivo 19F MRI for Cell Tracking

In vivo 19F MRI for Cell Tracking

In vivo 19 F MRI voor mobiele Tracking

In vivo 19F MRI allows quantitative cell tracking without the use of ionizing radiation. It is a noninvasive technique that can be applied to humans. ...

Carotid Artery Infusions for Pharmacokinetic and Pharmacodynamic Analysis of Taxanes in Mice

Halsslagader Infusions voor farmacokinetische en farmacodynamische analyse van Taxanen in Muizen

When proposing the use of a drug, drug combination, or drug delivery into a novel system, one must assess the pharmacokinetics of the drug in the study ...

Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo

Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo

Het evalueren van de effectiviteit van Cancer Drug Overgevoeligheid<em&gt; In Vitro</em&gt; En<em&gt; In Vivo</em

Due to the high level of heterogeneity and mutations inherent in human cancers, single agent therapies, or combination regimens which target the same ...

Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy

Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy

Validatie van Nanobody en antilichamen gebaseerde<em&gt; In Vivo</em&gt; Tumor Xenograft NIRF-imaging Experimenten op muizen, waarbij<em&gt; Ex Vivo</em&gt; Flowcytometrie en Microscopie

This protocol outlines the steps required to perform ex vivo validation of in vivo near-infrared fluorescence (NIRF) xenograft imaging experiments in mice ...

Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells

Uitputting van de Muis Cellen van geënte, humane tumoren verbetert Downstream Analyse van Target Cellen

The use of in vitro cell line models for cancer research has been a useful tool. However, it has been shown that these models fail to reliably mimic ...

Optimized Analysis of In Vivo and In Vitro Hepatic Steatosis

Optimized Analysis of In Vivo and In Vitro Hepatic Steatosis

Geoptimaliseerd Analyse van<em&gt; In Vivo</em&gt; en<em&gt; In Vitro</em&gt; Leversteatose

Establishing a system of procedures to qualitatively and quantitatively characterize in vivo and in vitro hepatic steatosis is important for metabolic ...

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells

voorbereiding en<em&gt; In Vitro</em&gt; Karakterisering van Magnetische miR-gemodificeerde endotheelcellen

To date, the available surgical and pharmacological treatments for cardiovascular diseases (CVD) are limited and often palliative. At the same time, gene ...

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice

In Vitro en In Vivo opsporing van Mitophagy in menselijke cellen, C. Elegansen muizen

Mitochondria are the powerhouses of cells and produce cellular energy in the form of ATP. Mitochondrial dysfunction contributes to biological aging and a ...

Preparation Of Gushukang (GSK) Granules for In Vivo and In Vitro Experiments

Preparation Of Gushukang GSK Granules for In Vivo and In Vitro Experiments

Bereiding van Gushukang (GSK) granulaat voor in vivo en in vitro experimenten

Traditional Chinese herbal medicine plays a role as an alternative method in treating many diseases, such as postmenopausal osteoporosis (POP). Gushukang ...

Use of a Linear Accelerator for Conducting In Vitro Radiobiology Experiments

Gebruik van een lineaire versneller voor het uitvoeren van in vitro radiobiologie experimenten

Radiation therapy remains one of the cornerstones of cancer management. For most cancers, it is the most effective, nonsurgical therapy to debulk tumors. ...