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DOI :
10.3791/55664-v
•
10:01 min
May 1st, 2017
Chapters
0:05
Title
0:52
Flash-and-Freeze
3:41
Automated Freeze-substitution Unit-transfer
6:40
Sample Mounting and Imaging
8:24
Results: Representative Exocytosis and Endocytosis in Mouse Hippocampal Neurons
8:56
Conclusion
我们开发的电子显微镜,“闪光和冻结,”一种新的技术,使膜动力学与毫秒的时间分辨率的可视化。该技术结合了高压冷冻神经元的光遗传学刺激。这里,我们证明的程序和详细描述了协议。
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