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DOI :
10.3791/55674-v
•
14:02 min
April 21st, 2017
Chapters
0:05
Title
13:18
Conclusion
11:32
Results: Glycopeptide Extraction and Analysis
1:05
Extraction of Extracellular Matrix Proteins
3:52
Protein Precipitation
4:57
Sequential Deglycosylation for the Assessment of N-glycosylation Site Occupancy
8:32
Glycopeptide Enrichment
6:05
Peptide Cleanup Using C18 Column
本文介绍一种方法来准备心血管组织样本进行质谱分析,允许(1)分析的ECM蛋白质组成,(2)识别的糖基化位,和(3)的组分特征的聚糖形式。这种方法可应用于进行微小的改进,在其他组织中的细胞外基质的研究。
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